Implications of the histological determination of microRNAs in the screening, diagnosis and prognosis of colorectal cancer

Menéndez, Pablo; Villarejo, Pedro; Padilla, David; Menéndez, José María; Rodríguez-Montes, José Antonio

doi:10.1002/jso.23344

Cited by 13 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, microRNAs differentially expressed in long and short survivors but not part of the signature are likely to contribute to the progression of the disease. This is reflected by the fact that many of the microRNAs identified in this study have been linked to development (Esquela‐Kerscher and Slack, 2006; Garzon et al., 2009; Ruan et al., 2009; Stahlhut and Slack, 2013) and prognosis (Kneitz et al., 2014; Menendez et al., 2013; Nair et al., 2012; Qu et al., 2014) of other cancers. For example miR‐21‐5p, miR‐221‐3p and members of the miR‐17∼92 cluster (miR‐17‐5p, miR‐19b‐3p, and miR‐20a‐5p) have been shown to regulate PTEN protein expression, and modulate the PI3K/Akt signaling axis (Concepcion et al., 2012; Pan et al., 2010).…”

Section: Discussionmentioning

confidence: 90%

MiR‐Score: A novel 6‐microRNA signature that predicts survival outcomes in patients with malignant pleural mesothelioma

et al. 2014

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 90%

MiR‐Score: A novel 6‐microRNA signature that predicts survival outcomes in patients with malignant pleural mesothelioma

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Recently, several studies have indicated that miRNA extracted from blood samples could be useful for the diagnosis of CRC [23, 24]. In addition, several studies have indicated that miRNAs may be potential biomarkers of either CRC metastases or recurrence [25, 26]. In the present study, we focused on the relationship between the expression of miRNA extracted from CRC tissue samples and its use as a predictive marker of recurrence.…”

Section: Discussionmentioning

confidence: 96%

High expression of miR-181c as a predictive marker of recurrence in stage II colorectal cancer

et al. 2016

View full text Add to dashboard Cite

INTRODUCTIONA standard treatment for stage II colorectal cancer (CRC) is surgical resection without adjuvant chemotherapy. However, the recurrence rate of these patients is approximately 20%. To date, there are no robust biomarkers suitable for predicting recurrence in stage II CRC patients. In this study, microRNAs (miRNAs) extracted from CRC tissues were examined for a possible biomarker to predict recurrence in stage II CRC patients.RESULTSFrom the comprehensive analysis, 15 miRNAs were selected as candidates for further study. Regarding let-7a, -7d, -7e, miR-23c, -26b, -128a, -151-5p, and -181c, recurrence rates in training cohort patients with higher expression of these miRNAs isolated from their frozen tissues samples were significantly higher than those with lower expression (P < 0.05). According to multivariate analysis, the higher expression of miR-181c was detected as an independent predictive factor of recurrence (P = 0.001, OR: 9.43, 95% CI: 2.57–34.48). Results were similar in miR-181c extracted from FFPE tissues obtained from the training cohort (P = 0.003, OR: 7.46, 95% CI: 1.97–28.57). In the validation cohort using FFPE tissues, the recurrence rate in patients with higher miR-181c expression was significantly higher than those with lower miR-181c expression (P < 0.001).MATERIAL AND METHODSComprehensive analysis using a highly sensitive miRNA chip was initially performed to select candidate miRNAs associated with recurrence. Candidate miRNAs were analyzed by real-time RT-PCR using RNA from frozen and formalin-fixed, paraffin-embedded (FFPE) tissues.CONCLUSIONSHigher expression of miR-181c may be a useful recurrence predictor of stage II CRC patients.

show abstract

“…Besides having a functional role in the initiation and progression of colorectal cancer, miRNAs are about to introduce a new era of diagnostic, prognostic and therapeutic modalities for this human malignancy (43)(44)(45). These small molecules are being intensively evaluated as biomarkers and therapeutic targets for colorectal cancer (46), as their expression patterns are subjected to remarkable alterations in tumor tissues (45), blood (plasma) (47,48) and feces (49)(50)(51). In addition to miRNA expression signatures (48), miRNA methylation signatures may provide candidate biomarkers for the detection of malignant colonocytes, since miRNAs are epigenetically modified in CRC (52,53).…”

Section: Discussionmentioning

confidence: 99%

miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma

Adamopoulos

Kontos

Rapti

et al. 2014

International Journal of Oncology

View full text Add to dashboard Cite

Colorectal adenocarcinoma constitutes the most frequent form of colorectal cancer and a serious cause of cancer-related deaths. The expression of multiple miRNAs, including miR-224, is deregulated in colorectal adenocarcinoma. The aim of this study was the investigation of the prognostic value of miR-224 in colorectal adenocarcinoma. For this purpose, total RNA was isolated from 115 colorectal adenocarcinomas and 66 adjacent non-cancer mucosae. Total RNA (2 µg) was polyadenylated and reverse transcribed. A quantitative PCR method based on SYBR-Green chemistry was developed and applied for the quantification of miR-224 levels, followed by extensive biostatistical analysis. miR-224 levels in malignant colorectal adenocarcinomas ranged between 1.81 and 187.75 RQU (miR-224 copies/1,000 SNORD48 copies) with a median of 34.27, and were significantly elevated, compared to miR-224 levels in adjacent non-cancer mucosae (p<0.001). Enhanced miR-224 expression constitutes a rather strong prognosticator in colorectal adenocarcinoma, predicting short-term relapse and poor overall survival in these patients (p=0.012 and p=0.005, respectively), independent of established clinicopathological parameters. In conclusion, miR-224 is significantly upregulated in malignant colorectal tumors compared to adjacent non-cancer mucosae, and its enhanced expression constitutes an independent predictor of short-term relapse and poor overall survival in colorectal adenocarcinoma patients.

show abstract

Implications of the histological determination of microRNAs in the screening, diagnosis and prognosis of colorectal cancer

Cited by 13 publications

References 38 publications

MiR‐Score: A novel 6‐microRNA signature that predicts survival outcomes in patients with malignant pleural mesothelioma

MiR‐Score: A novel 6‐microRNA signature that predicts survival outcomes in patients with malignant pleural mesothelioma

High expression of miR-181c as a predictive marker of recurrence in stage II colorectal cancer

miR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma

Contact Info

Product

Resources

About