Implications of aspect ratio on the uptake and nanotoxicity of gold nanomaterials

Abtahi, Seyyed Mohammad Hossein; Trevisan, Rafael; Giulio, Richard Di; Murphy, Catherine J.; Saleh, Navid B.; Vikesland, Peter J.

doi:10.1016/j.impact.2019.100153

Cited by 10 publications

(5 citation statements)

References 74 publications

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“…However, 12 metabolites showed differences only in the GNR-S group, indicating that GNR-S caused additional effects on Hep G2 cells. This may have been due to the smaller volume of GNR-S, which could facilitate penetration of the cells’ protective barrier to produce other toxic effects [ 43 ]. Z-score plots were constructed to display the statistically significant metabolites in the three GNR-dosed groups, as shown in Figure 2 i–k.…”

Section: Resultsmentioning

confidence: 99%

Cell-Based Metabolomics Approach for Anticipating and Investigating Cytotoxicity of Gold Nanorods

Sun

Zhang

et al. 2022

Foods

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Despite the increasing application of gold nanoparticles, there has been little assessment of biological system toxicity to evaluate their potential impact on human health. In this study, the human hepatoma cell line (Hep G2) was used in a metabolomics approach to study the effects of shape, time, and dose of gold nanorods (GNRs). Using optimized parameters for chromatography and mass spectrometry, the metabolites detected by GC-MS were processed with MS DIAL and identified with Fiehnlib. Key metabolic pathways affected by GNRs were identified by endo-metabolic profiling of cells mixed with GNRs of varying shape while varying the dose and time of exposure. The shape of GNRs affected cytotoxicity, and short GNR (GNR−S) triggered disorder of cell metabolism. High concentrations of GNRs caused more significant toxicity. The cytotoxicity and bioTEM results illustrated that the mitochondria toxicity, as the main cytotoxicity of GNRs, caused declining cytoprotective ability. The mitochondrial dysfunction disrupted alanine, aspartate, glutamate, arginine, and proline metabolism, with amino acid synthesis generally downregulated. However, the efflux function of cells can exclude GNRs extracellularly within 24 h, resulting in reduced cell mitochondrial metabolic toxicity and allowing metabolic disorders to recover to normal function.

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Section: Resultsmentioning

confidence: 99%

Cell-Based Metabolomics Approach for Anticipating and Investigating Cytotoxicity of Gold Nanorods

Sun

Zhang

et al. 2022

Foods

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“…The remarkable biological, chemical, and physical reactivity of ENMs determines their environmental transformations and allows them to interact with marine organisms through different exposure routes, including direct/indirect ingestion [ 21 , 125 ], contact with gills [ 126 , 127 ] and/or body adsorption [ 34 , 128 , 129 ].…”

Section: Current Knowledge On Enms Marine Ecotoxicitymentioning

confidence: 99%

Eco-Interactions of Engineered Nanomaterials in the Marine Environment: Towards an Eco-Design Framework

et al. 2021

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Marine nano-ecotoxicology has emerged with the purpose to assess the environmental risks associated with engineered nanomaterials (ENMs) among contaminants of emerging concerns entering the marine environment. ENMs’ massive production and integration in everyday life applications, associated with their peculiar physical chemical features, including high biological reactivity, have imposed a pressing need to shed light on risk for humans and the environment. Environmental safety assessment, known as ecosafety, has thus become mandatory with the perspective to develop a more holistic exposure scenario and understand biological effects. Here, we review the current knowledge on behavior and impact of ENMs which end up in the marine environment. A focus on titanium dioxide (n-TiO2) and silver nanoparticles (AgNPs), among metal-based ENMs massively used in commercial products, and polymeric NPs as polystyrene (PS), largely adopted as proxy for nanoplastics, is made. ENMs eco-interactions with chemical molecules including (bio)natural ones and anthropogenic pollutants, forming eco- and bio-coronas and link with their uptake and toxicity in marine organisms are discussed. An ecologically based design strategy (eco-design) is proposed to support the development of new ENMs, including those for environmental applications (e.g., nanoremediation), by balancing their effectiveness with no associated risk for marine organisms and humans.

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“…Bio–nano interface issues have been studied extensively in the context of drug delivery in situ , in vitro , and in vivo . − They are less well understood for the performance and reproducibility of paper-based immunoassays. In immunoassays, the bio–nano interface issues are arguably even more significant, where interface effects for both the NP–antibody conjugate and also the nitrocellulose substrate can dominate the assay behavior. − Here, we study the performance of the immunoprobes in paper dipstick immunoassays and how it is impacted by the immunoprobe synthesis strategy and surface architecture.…”

Section: Introductionmentioning

confidence: 99%

The Immunoprobe Aggregation State is Central to Dipstick Immunoassay Performance

Hristov

Pimentel

Ujialele

et al. 2020

ACS Appl. Mater. Interfaces

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As new infectious disease outbreaks become more likely, it is important to be able to develop and deploy appropriate testing in time. Paper-based immunoassays are rapid, cheap, and easy to produce at scale and relatively user friendly but often suffer from low selectivity and sensitivity. Understanding the molecular mechanisms of paper immunoassays may help improve and hasten development and therefore production and market availability. Here, we study how the behavior of nanoparticle−antibody immunoprobes in paper dipstick immunoassays is impacted by synthesis strategy and surface chemistry architecture. We conjugate gold nanoparticles to polyclonal anti-immunoglobulin G (IgG) and anti-zika NS1 antibodies by electrostatic adsorption and N-hydroxysuccinimide (NHS) and hydrazide (Hz) chemistries. The immunoprobes were used in paper immunoassays and the effective affinity for the antigen was quantified from the test line intensities, as well as the distribution of the immunoprobes throughout the strips. The results show that nanoparticle colloidal stability, both post synthesis and during antigen binding, is a key factor and affects immunoassay results and performance, often through reduction or loss of signal.

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Implications of aspect ratio on the uptake and nanotoxicity of gold nanomaterials

Cited by 10 publications

References 74 publications

Cell-Based Metabolomics Approach for Anticipating and Investigating Cytotoxicity of Gold Nanorods

Cell-Based Metabolomics Approach for Anticipating and Investigating Cytotoxicity of Gold Nanorods

Eco-Interactions of Engineered Nanomaterials in the Marine Environment: Towards an Eco-Design Framework

The Immunoprobe Aggregation State is Central to Dipstick Immunoassay Performance

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