Implications of anticoagulants and gender on cell counts and growth factor concentration in platelet-rich plasma and platelet-rich gel supernatants from rabbits

Gonzalez, Juan Carlos; López, Catalina; Carmona, JU

doi:10.3415/vcot-15-01-0011

Cited by 14 publications

(12 citation statements)

References 54 publications

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“…Conditions affecting the quality and effects of PRP include: the age, sex, and pathophysiology of blood donors during PRP preparation; methods of drawing blood; anticoagulants; centrifugation time/speed/temperature; preservation method of PRP; interval and dose of use.…”

Section: Discussionmentioning

confidence: 99%

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Tian

Cheng

Chen

et al. 2019

Wound Repair Regeneration

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In recent years, autologous platelet-rich plasma (PRP) derivatives have been used widely in the regeneration and repair of tissue, but a standard definition and preparation method for PRP are lacking. We developed a standardized method using platelet indices as quality-control indicators for PRP preparation. Twenty-one elderly patients (9 males, 12 females) with complex wounds were treated with standardized platelet-rich plasma (S-PRP). The platelet count in PRP after the second centrifugation was 1,069-1,436 × 10 9 /L. We adjusted the platelet concentration in PRP after a second centrifugation to 1,000 × 10 9 /L according to a formula using platelet-poor plasma (PPP). The standardized preparation method that we developed gave S-PRP with a relatively uniform platelet concentration. The wounds of 21 patients showed accelerated healing after S-PRP treatment, and there were no obvious side effects during treatment. These data suggest that our preparation method of S-PRP, using platelet indices as quality-control indicators with platelet count of 1,000 × 10 9 /L could be used for the treatment of complex wounds in the elderly. The preparation method of S-PRP proposed in the present study may be a simple and effective method of PRP quality control.

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Section: Discussionmentioning

confidence: 99%

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Tian

Cheng

Chen

et al. 2019

Wound Repair Regeneration

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“…This last type of PRP is proposed as a PRP product characterized by a low count of WBCs (0.3–0.5 times the WBC concentration in whole blood) and a PLT concentration similar or slightly lower or higher in relation to the PLT concentration in whole blood [33]. At this point, it remains necessary to evaluate these bovine PRPs in in vitro systems and under clinical conditions affecting cattle.…”

Section: Discussionmentioning

confidence: 99%

“…This was an unexpected biological phenomenon, because this GF is regularly present in lower concentrations in plasma and in higher concentrations in PRG supernatants and their correspondent PRP lysates in mammals, like horses [15, 17, 43], cats [44], and rabbits [33]. Two technical or biological explanations could explain this finding: (1) human PDGF-BB specific antibodies were used for capturing bovine PDGF-BB.…”

Section: Discussionmentioning

confidence: 99%

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Gutiérrez

López

Giraldo

et al. 2017

Veterinary Medicine International

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There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs.

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“…An animal model study, aiming to investigate the influence of sodium citrate and ACD-solution A anticoagulants on cell count and growth factor concentration in pure platelet-rich gel supernatants, reported an increased number of platelets and leukocytes in sodium citrate PRP compared to homologous acid-citrate-dextrose solution A PRP fraction, but no difference concerning growth factor concentration [31].…”

Section: Anticoagulantsmentioning

confidence: 99%

Platelet Concentrates in Musculoskeletal Medicine

Mariani

Pulsatelli

2020

IJMS

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Platelet concentrates (PCs), mostly represented by platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous biological blood-derived products that may combine plasma/platelet-derived bioactive components, together with fibrin-forming protein able to create a natural three-dimensional scaffold. These types of products are safely used in clinical applications due to the autologous-derived source and the minimally invasive application procedure. In this narrative review, we focus on three main topics concerning the use of platelet concentrate for treating musculoskeletal conditions: (a) the different procedures to prepare PCs, (b) the composition of PCs that is related to the type of methodological procedure adopted and (c) the clinical application in musculoskeletal medicine, efficacy and main limits of the different studies.

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Implications of anticoagulants and gender on cell counts and growth factor concentration in platelet-rich plasma and platelet-rich gel supernatants from rabbits

Abstract: The type of anticoagulant and gender affected the cell counts in PRP, but they did not influence the growth factor concentration. More complete rabbit PRP studies should be performed before evaluating this type of substance in models of disease.

Cited by 14 publications

References 54 publications

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Platelet Concentrates in Musculoskeletal Medicine

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