Implications for the use of acid preservatives in 24-hour urine for measurements of high demand biochemical analytes in clinical laboratories

Feres, Márcia Cristina; Bini, Rolando; Martino, Maria Cristina De; Biagini, Simone P.; Sousa, Altay Lino de; Campana, Paulo G.; Tufik, Sérgio

doi:10.1016/j.cca.2011.08.033

Cited by 18 publications

(8 citation statements)

References 16 publications

(22 reference statements)

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“…There are many different preservative methods, but a universal preservative allowing complete urinalysis does not exist (57,61 ). Addition of preservatives and types of preservatives may change urine volume and give rise to potential interference with assay methods (44,57 ).…”

Section: Urinementioning

confidence: 99%

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Ellervik

Vaught²

2015

Clinical Chemistry

137

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BACKGROUND Most errors in a clinical chemistry laboratory are due to preanalytical errors. Preanalytical variability of biospecimens can have significant effects on downstream analyses, and controlling such variables is therefore fundamental for the future use of biospecimens in personalized medicine for diagnostic or prognostic purposes. CONTENT The focus of this review is to examine the preanalytical variables that affect human biospecimen integrity in biobanking, with a special focus on blood, saliva, and urine. Cost efficiency is discussed in relation to these issues. SUMMARY The quality of a study will depend on the integrity of the biospecimens. Preanalytical preparations should be planned with consideration of the effect on downstream analyses. Currently such preanalytical variables are not routinely documented in the biospecimen research literature. Future studies using biobanked biospecimens should describe in detail the preanalytical handling of biospecimens and analyze and interpret the results with regard to the effects of these variables.

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Section: Urinementioning

confidence: 99%

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Ellervik

Vaught²

2015

Clinical Chemistry

137

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“…En nuestro estudio, la mayoría de laboratorios 89,3% no utiliza conservantes para ningún tipo de determinación, lo que se traduce en una falta de armonización por parte de los laboratorios para el uso de dichos aditivos (1) . Algunos estudios revelan que para ciertos constituyentes bioquímicos, el uso de preservantes en orina de 24 horas no es necesario (16,17) .…”

Section: Discussionunclassified

Errores preanalíticos en la recolección de orina de 24 horas

Orejón

2021

An Fac med

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Introducción. La recolección de orina en el lapso de 24 horas es necesaria para la medición de constituyentes bioquímicos que presentan una excreción urinaria variable; sin embargo, dicho proceso no está exento de errores preanalíticos. Objetivos. Evaluar el nivel de cumplimiento de las instrucciones para recoger la muestra, identificar la recolección de orinas incompletas y conocer los procesos preanalíticos que aplica un grupo de profesionales. Métodos. Estudio de diseño observacional, en el que se realizó encuestas anónimas y voluntarias a 257 pacientes ambulatorios y 59 profesionales tecnólogos médicos, además de estimar la excreción urinaria de creatinina a 416 muestras. Resultados. El estudio evidenció recolecciones incorrectas (39,7%), uso de recipientes inadecuados (58,14%), mala conservación de las muestras (98,8%), cambio en los hábitos de ingesta de líquidos (23,7%), escasa información y retroalimentación a los pacientes sobre la preparación de la prueba, el 76,92% de muestras presentaron pérdidas o excesos en el volumen recolectado y una alta variabilidad de los criterios que utilizan los profesionales para rechazar las muestras mal colectadas. Conclusión. Se observó un bajo cumplimiento de los pacientes a las indicaciones para la recolección de la muestra, una alta proporción de recolecciones incompletas y discordancia en los procesos preanalíticos para el análisis de orina de 24 horas.

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“…The samples were collected in the morning at 9 AM, after a 12-hour fasting period and 1 hour of rest. Twenty four hour urine was collected from participants and then acidified with 5 mL of 6N HCl prior to the tests [33].…”

Section: Methodsmentioning

confidence: 99%

Evaluation and Validation of a Method for Determining Platelet Catecholamine in Patients with Obstructive Sleep Apnea and Arterial Hypertension

et al. 2014

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BackgroundMeasurements of plasma and urinary catecholamine are susceptible to confounding factors that influence the results, complicating the interpretation of sympathetic nervous system (SNS) activity in the Obstructive sleep apnea (OSA) and arterial hypertension (HYP) conditions.ObjectiveIn this study, we validated a test for platelet catecholamine and compared the catecholamine levels (adrenaline and noradrenaline) in urine, plasma and platelets in patients with OSA and HYP compared with controls.MethodsIn the validation, 30 healthy, nonsmoking volunteers who were not currently undergoing treatment or medication were selected as the control group. One hundred fifty-four individuals (114 OSA, 40 non-OSA) were consecutively selected from the outpatient clinic of the Sleep Institute and underwent clinical, polysomnographic and laboratory evaluation, including the urinary, plasma and platelet levels of adrenaline (AD) and noradrenaline (NA). Patients were then allocated to groups according to the presence of OSA and/or hypertension.ResultsA logistic regression model, controlled for age and BMI, showed that urinary AD and urinary NA were risk factors in the OSA+HYP group and the HYP group; however, the model showed higher levels of platelet NA for OSA without HYP. After 1 year of CPAP (continuous upper airway pressure) treatment, patients (n = 9) presented lower levels of urinary NA (p = 0.04) and platelet NA (p = 0.05).ConclusionUrinary NA and AD levels were significantly associated with the condition of hypertension with and without OSA, whereas platelet NA with OSA without comorbidity. These findings suggest that platelet catecholamine levels might reflect nocturnal sympathetic activation in OSA patients without hypertension.

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Implications for the use of acid preservatives in 24-hour urine for measurements of high demand biochemical analytes in clinical laboratories

Abstract: Glucose, microalbumin, protein, amylase and aldosterone urinary did not show good performance in the presence of acid preservative. Analytes that need acid preservative showed variation acceptable in condition 5 ml/l of 6 mol/l HCl added after 24 h.

Cited by 18 publications

References 16 publications

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

Errores preanalíticos en la recolección de orina de 24 horas

Evaluation and Validation of a Method for Determining Platelet Catecholamine in Patients with Obstructive Sleep Apnea and Arterial Hypertension

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