Implication of the mineralocorticoid axis in rainbow trout osmoregulation during salinity acclimation

Kiilerich, Pia; Milla, Sylvain; Sturm, Armin; Valotaire, Claudiane; Chevolleau, Sylvie; Giton, Frank; Terrien, Xavier; Fiet, Jean; Fostier, Alexis; Debrauwer, Laurent; Prunet, Patrick

doi:10.1530/joe-10-0371

Cited by 47 publications

(34 citation statements)

References 52 publications

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“…This suggests, together with co-localization of the MR with GR1 in transporting epithelial cells such as intestinal, gill and kidney cells in trout (Kiilerich et al 2011a), that cortisol signaling in fish could be mediated by both homo-and heterodimerization of the corticosteroid nuclear receptors, as shown in mammals.…”

mentioning

confidence: 78%

“…Proteins were transferred by submerged blotting to an Amersham Hybond-C Extra membrane (GE Healthcare, Little Chalfont, Buckinghamshire, UK) before blocking for 2 h at RT in Tris-buffered salineCTween (TBST) with 5% milk. The membranes were incubated overnight at 4 8C with primary antibodies (rabbit anti-rtMR 1/1000 dilution (Kiilerich et al 2011a); rabbit anti-rtGR1 1/2000 dilution (Tujague et al 1998), mouse anti-GFP 1/2000 dilution (JL-8, Clontech) and mouse anti-b-actin 1/3000 dilution (sc-47778, Santa Cruz Biotechnology). After washing in TBST, the membranes were incubated for 1 h at RT with a secondary antibody (anti-rabbit-HRP and/or anti-mouse-HRP in 1/20 000 dilution in TBST C5% milk) with a StrepTactin-HRP anti-protein marker antibody (1/5000 dilution, Bio-Rad).…”

Section: Western Blottingmentioning

confidence: 99%

“…Due to the lack of 11b-hydroxylase, aldosterone synthesis is absent in fish (Jiang et al 1998), seemingly leaving the MR with only cortisol as a ligand. However, the aldosterone synthesis intermediate 11-deoxycorticosterone (DOC), which is present in rainbow trout plasma in the nanomolar range (Milla et al 2008, Kiilerich et al 2011a, specifically binds and activates the MR with higher affinity than cortisol (Sturm et al 2005). Although the biological roles of DOC in humans and other vertebrates have recently been reconsidered (reviewed by Vinson (2011)), the physiological relevance of DOC in osmoregulation in fish is still not established (Kiilerich et al 2007, 2011a, b, c, McCormick et al 2008.…”

mentioning

confidence: 99%

“…However, the aldosterone synthesis intermediate 11-deoxycorticosterone (DOC), which is present in rainbow trout plasma in the nanomolar range (Milla et al 2008, Kiilerich et al 2011a, specifically binds and activates the MR with higher affinity than cortisol (Sturm et al 2005). Although the biological roles of DOC in humans and other vertebrates have recently been reconsidered (reviewed by Vinson (2011)), the physiological relevance of DOC in osmoregulation in fish is still not established (Kiilerich et al 2007, 2011a, b, c, McCormick et al 2008. Notably, concurrent with the high expression and localization of the MR in the mammalian brain, the MR and DOC have recently been suggested to be involved in the regulation of fish behavior (Sakamoto et al 2011).…”

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confidence: 99%

“…Whole-genome duplication in the teleost lineage w350 MYA gave rise to two GR isoforms, GR1 and GR2, with an identical domain structure and many similarities in terms of ligand-and DNA-binding (reviewed by Bury & Sturm (2007) and Li et al (2012)). Differential tissue mRNA expression levels and regulation of expression (Greenwood et al 2003, Stolte et al 2008a, Kiilerich et al 2011a, Trayer et al 2013, together with dissimilar EC 50 values for cortisol (Bury et al 2003, Greenwood et al 2003, Stolte et al 2008b, Kim et al 2011, Li et al 2012, suggest functional differences between these two GR subtypes.…”

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confidence: 99%

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Interaction between the trout mineralocorticoid and glucocorticoid receptors in vitro

Kiilerich¹,

Triqueneaux²,

Christensen³

et al. 2015

Journal of Molecular Endocrinology

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The salmonid corticosteroid receptors (CRs), glucocorticoid receptors 1 and 2 (GR1 and GR2) and the mineralocorticoid receptor (MR) share a high degree of homology with regard to structure, ligand-and DNA response element-binding, and cellular co-localization. Typically, these nuclear hormone receptors homodimerize to confer transcriptional activation of target genes, but a few studies using mammalian receptors suggest some degree of heterodimerization. We observed that the trout MR confers a several fold lower transcriptional activity compared to the trout GRs. This made us question the functional relevance of the MR when this receptor is located in the same cells as the GRs and activated by cortisol. A series of co-transfection experiments using different glucocorticoid response elements (GREs) containing promoter-reporter constructs were carried out to investigate any possible interaction between the piscine CRs. Co-transfection of the GRs with the MR significantly reduced the total transcriptional activity even at low MR levels, suggesting interaction between these receptors. Co-transfection of GR1 or GR2 with the MR did not affect the subcellular localization of the GRs, and the MR-mediated inhibition seemed to be independent of specific activation or inhibition of the MR. Site-directed mutagenesis of the DNA-binding domain and dimerization interface of the MR showed that the inhibition was dependent on DNA binding but not necessarily on dimerization ability. Thus, we suggest that the interaction between MR and the GRs may regulate the cortisol response in cell types where the receptors co-localize and propose a dominant-negative role for the MR in cortisolmediated transcriptional activity.

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confidence: 78%

Section: Western Blottingmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Interaction between the trout mineralocorticoid and glucocorticoid receptors in vitro

Kiilerich¹,

Triqueneaux²,

Christensen³

et al. 2015

Journal of Molecular Endocrinology

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Environmental concentrations of agricultural‐use pesticide mixtures evoke primary and secondary stress responses in rainbow trout

Tierney¹,

Williams²,

Gledhill³

et al. 2011

Enviro Toxic and Chemistry

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The present study sought to determine whether environmentally realistic mixtures of agriculturally important pesticides are stressful to fish. Juvenile rainbow trout were exposed for 96 h to concentrations of a pesticide mixture found in a waterway that is the focus of salmon restoration efforts (Nicomekl River, BC, Canada). This mixture contained organochlorine, organophosphorus, phenylurea, and triazine classes of pesticides. Fish given a realistic mixture exposure (total concentration, 1.01 µg/L) had increased plasma cortisol concentration, packed red cell volume, hematocrit (Hct), as well as decreased white cell volume, leukocrit (Lct). Similar changes in Hct and Lct were apparent after exposure to a lower concentration (0.186 µg/L). Interestingly, no changes in plasma cortisol concentration, Hct, or Lct were noted after exposure to a higher concentration (13.9 µg/L). This suggests that the exposure likely impaired the mechanisms enabling the stress response. Across all exposures, plasma glucose concentration was related to plasma cortisol concentration, not to pesticide mixture concentration. This suggests that a secondary stress response may be more related to variability in individual primary stress response than to differences in pesticide exposure concentrations. In summary, the present study indicates that salmon living in agrichemical-contaminated waterways may be experiencing stress, and this may pose a threat to their survival.

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Cortisol stimulates arginine vasotocin and isotocin release from the hypothalamo‐pituitary complex of round goby (Neogobius melanostomus): Probable mechanisms of action

2015

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There were two aims of this in vitro perfusion study. Firstly, to determine which class of receptors, glucocorticoid (GRs) or mineralocorticoid (MRs), are involved in cortisol regulation of arginine vasotocin (AVT) and isotocin (IT) release from the hypothalamo-pituitary (H-P) complex of round goby (Neogobius melanostomus). Secondly, to determine which pathways, genomic or non-genomic, are involved in the aformentioned process.The H-P explants were perfused with cortisol (1.4 × 10(-) (7) M, 2.8 × 10(-7) M, 0.4 × 10(-6) M); only the highest dose significantly increased a release of both nonapeptides. In the perfusion of H-P explants, we used cortisol (0.4 × 10(-6) M) in combination with GRs antagonist RU486 (0.3 × 10(-6) M) or MRs antagonist C03DA01 (0.36 × 10(-6) M) or transcription inhibitor Actinomycin D (1 × 10(-7) M). All inhibitors were also tested seperately. The contents of AVT and IT in the perfusion media was determined by high-performance liquid chromatography (HPLC) with UV detection. This study suggested that different mechanisms were involved in the regulation of AVT and IT release from H-P complex in round goby. Apparently it was GRs but not MRs that were involved in cortisol regulation of AVT and IT release. In the case of AVT, our data points to both genomic and non-genomic pathways mediating the effect of cortisol; in the case of IT, it is only the non-genomic pathway. This study presents the first feasible mechanisms of cortisol action on AVT and IT release from the H-P complex in round goby.

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Implication of the mineralocorticoid axis in rainbow trout osmoregulation during salinity acclimation

Cited by 47 publications

References 52 publications

Interaction between the trout mineralocorticoid and glucocorticoid receptors in vitro

Interaction between the trout mineralocorticoid and glucocorticoid receptors in vitro

Environmental concentrations of agricultural‐use pesticide mixtures evoke primary and secondary stress responses in rainbow trout

Cortisol stimulates arginine vasotocin and isotocin release from the hypothalamo‐pituitary complex of round goby (Neogobius melanostomus): Probable mechanisms of action

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