Implication of phospholipase D in response of Hordeum vulgare root to short-term potassium deprivation

Abstract

SummaryTo verify a possible implication of lipids and some other compounds such as hydrogen peroxide (H 2 O 2 ) and glyceraldehydes-3-phosphate dehydrogenase (G3PDH) in the response of Hordeum vulgare to an early potassium deprivation, plants were grown in hydroponics for 30 days with a

Rice and chickpea GDPDs are preferentially influenced by low phosphate and CaGDPD1 encodes an active glycerophosphodiester phosphodiesterase enzyme

“…This increase in root growth was correlated with increased PA accumulation in roots ). Similarly, potassium deficiency also led to early accumulation of PA within 24 h of potassium deprivation in Hordeum vulgare (Hafsi et al 2009). Though, the overall total lipid content did not change, specific hydrolysis of phospholipid species was observed under K deprivation (Hafsi et al 2009).…”

“…Similarly, potassium deficiency also led to early accumulation of PA within 24 h of potassium deprivation in Hordeum vulgare (Hafsi et al 2009). Though, the overall total lipid content did not change, specific hydrolysis of phospholipid species was observed under K deprivation (Hafsi et al 2009). Thus, upregulation of OsGDPDs after 7 days of different nutrient deficiencies might play a role in regulating PA homeostasis within cell for controlling root growth or root hair formation.…”

“…Further, Arabidopsis mutants of dgd1, pah1pah2, sqd2 also showed impaired growth upon P starvation (Nakamura 2013). Though originally characterized under P starvation, some lipid remodeling genes/enzymes have also been shown to play important roles under other nutrient stresses (Abida et al 2015;Gaude et al 2007;Hafsi et al 2009;Hong et al 2009;Narasimhan et al 2013;Rietz et al 2004;Urzica et al 2013). Apart from the key genes for phospholipid degradation pathway, a less explored alternative pathway of phospholipid hydrolysis involves GDPD which degrade intermediate product of phospholipid degradation, glycerophosphodiesters to G3P.…”

“…However, till now the role of GDPDs in other nutrient stresses is largely unspecified. Although some studies have shown a role of lipid remodeling genes in signaling, root growth, biomass accumulation and alteration of membrane lipid compositions in nutrient deficiencies other than P (Gaude et al 2007;Hafsi et al 2009;Hong et al 2009;Narasimhan et al 2013;Urzica et al 2013), yet evidence about specific role of GDPDs is missing. Therefore, in the current study, we investigated the role of GDPDs under different nutrient deficiencies in rice (Oryza sativa) and chickpea (Cicer arietinum) crops.…”

Molecular cloning and partial characterization of a novel phospholipase D gene from Chorispora bungeana

“…In vivo and in vitro studies in suspension-cultured rice cells, PLD was activated by H 2 O 2 and this activation involved a protein tyrosine kinase (Yamaguchi et al 2004). Hafsi et al (2009) reported that the eventual PLD activation may be the result of G3PDH inhibition by H 2 O 2 . It was also observed that stress-stimulated production of ROS (H 2 O 2 ) activates plasma membrane-associated PLD delta through an increase in the cytosolic Ca 2?…”