Implication of human papillomavirus-66 in vulvar carcinoma: a case report

Kotsopoulos, Ioannis C; Tampakoudis, Georgios P; Evaggelinos, Dimitrios; Nikolaidou, Anastasia; Fytili, Panagiota A; Kartsiounis, V; Gerasimidou, Domniki

doi:10.1186/1752-1947-5-232

Cited by 3 publications

(2 citation statements)

References 11 publications

(8 reference statements)

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“…HPV66 is an α-papillomavirus that infect mucosal membranes and belong to the high risk HPV group. [25]. The presence of HPV66 genotype in the Egyptian patients, as an interesting nding of this study, might be related to the geographical region.…”

Section: Discussionmentioning

confidence: 67%

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Salem¹,

Bahaa

Rouby

et al. 2020

Preprint

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Background: The new classification of Head and Neck Squamous Cell Carcinoma (HNSCC) according to their association with human papillomavirus (HPV) infection into HPV positive tumors with good prognosis and HPV negative tumors with poor prognosis has changed the treatment strategy of HNSCCs dramatically worldwide. However, data concerning HPV infection in HNSCCs patients is still limited and there are no studies regarding HPV genotypes in HNSCC among Egyptians. Therefore, the aim of the current study is to investigate the frequency of HPV and to detect the most prevalent HPV genotypes in the patients with oral cavity and oropharyngeal carcinoma from EgyptMethods: This cross sectional study was conducted from January 2016 to November 2017. Ninety-two oral brushing samples (76 oral cavity and 16 oropharynx) were collected by cytobrushing in CYTO-fast solution. HPV-DNA was subjected to two methods which are the: (1) HPV Direct Flow CHIP system for automatic detection and genotyping (2) conventional PCR for detection of HPV using consensus primers (MYO9/MYO11). SPSS software was used for statistical analysis.Results: Out of the 92 cases assessed, HPV DNA was present in three samples only (3/92; 3.3%) and the HPV66 genotype was the only genotype detected by HPV Direct Flow CHIP System.Conclusion: The Results of this study may (1) indicate that the HPV-associated HNSCC among Egyptian patients is very low and (2) highlight the presence of HPV66 genotype in these malignancies. Other studies from other countries in general and from different governorates in Egypt are still needed to determine the exact frequency of HPV infections in head and neck cancer.

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Section: Discussionmentioning

confidence: 67%

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Salem¹,

Bahaa

Rouby

et al. 2020

Preprint

View full text Add to dashboard Cite

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“… 28 Although the prevalence and distribution of HPV-66 in most studies have depended highly upon the origin of the population involved, in a meta-analysis of carcinomas and intra-epithelial neoplasia of the vulva, vagina, and anus, HPV-66, among other rare types, was found in no more than 0.5% of the anogenital carcinomas tested. 28 , 29 However, some literatures report that the inclusion of HPV-66 in the HPV Array Test is an ongoing source of false-positive results. 7 , 8 HPV-66 was detected on the vulvar biopsy of the patient in this case report.…”

Section: Discussionmentioning

confidence: 99%

The Importance of p16 and p53 Immunohistochemical Staining in Diagnosing Vulvar Lichen Simplex Chronicus Mimicking Vulvar Intraepithelial Neoplasia with False-Positive Human Papillomavirus Type 66

Achdiat,

Widjaya,

Rowawi

et al. 2024

IJWH

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Vulvar intraepithelial neoplasia (VIN), the precursor lesion of vulvar squamous cell carcinoma (VSCC), may present as pruritic or asymptomatic lichenified plaques surrounded by single or multiple discrete or confluent macules or papules. VIN is divided into high-grade squamous intraepithelial lesion (HSIL), which is human papillomavirus (HPV)-driven, and differentiated VIN (DVIN), which develops independently of HPV. Histopathological examination and HPV genotyping polymerase chain reaction (PCR) tests should be performed to distinguish between HSIL and DVIN. Lichenified plaques surrounded by multiple papules are found not only in VIN but also in vulvar lichen simplex chronicus (LSC). This chronic inflammatory skin disease mostly appears in labia majora and is triggered by sweating, rubbing, and mental stress. IHC staining of p16 and p53 are recommended as the most commonly used biomarkers for VIN in diagnostically challenging cases. IHC staining is also beneficial to confirm the accuracy of the HPV detection technique, as p16-negative staining may also represent a false-positive result. We report a case of the importance of p16 and p53 IHC staining in diagnosing vulvar LSC mimicking VIN with false-positive HPV-66. The patient was previously diagnosed with VIN based on clinical examination. HPV-66 was detected by PCR from a vulvar biopsy sample. Histopathological examination revealed stromal lymphocytic infiltration with non-specific chronic dermatitis; neither atypia nor koilocyte was observed. Both p16 and p53 IHC staining were negative. The patient was diagnosed and treated as vulvar LSC with 10 mg cetirizine tablet, emollient, and 0.1% mometasone furoate cream. Clinical improvement was observed as the lesions became asymptomatic hyperpigmented macules in the 4 weeks of follow-up, without recurrence after 3 years of follow-up. Both p16 and p53 IHC staining might help distinguish HSIL and DVIN mutually and from other vulvar mimics in diagnostically challenging cases.

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Molecular Diagnosis of Cervical Cancer

Mittal

Yadav

2019

Molecular Diagnostics in Cancer Patients

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Implication of human papillomavirus-66 in vulvar carcinoma: a case report

Cited by 3 publications

References 11 publications

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

The Importance of p16 and p53 Immunohistochemical Staining in Diagnosing Vulvar Lichen Simplex Chronicus Mimicking Vulvar Intraepithelial Neoplasia with False-Positive Human Papillomavirus Type 66

Molecular Diagnosis of Cervical Cancer

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