Implication of Fusarium graminearum primary metabolism in its resistance to benzimidazole fungicides as revealed by 1H NMR metabolomics

Sevastos, A.; Kalampokis, Ioannis F.; Panagiotopoulou, Antigoni; Pelecanou, Maria

doi:10.1016/j.pestbp.2018.03.015

Cited by 36 publications

(24 citation statements)

References 74 publications

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“…Metabolomics is a fast-growing and high-throughput platform for bioanalysis applied in studying MoAs of many microbes such as yeast, Saccharomyces cerevisiae (Allen et al, 2004), and pathogenic fungi (Aliferis and Jabaji, 2011;Sevastos et al, 2018). This method has unique advantages over genomics, transcriptomics, and proteomics because the metabolome indicates what is actually happening in a biological system, thus serving as a link between genome and phenome (Fiehn, 2002;Wiklund et al, 2008;Carreno-Quintero et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

et al. 2020

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The fungicide SYP-14288 has a high efficiency, low toxicity, and broad spectrum in inhibiting both fungi and oomycetes, but its mode of action (MoA) remains unclear on inhibiting fungi. In this study, the MoA was determined by analyzing the metabolism and respiratory activities of Rhizoctonia solani treated by SYP-14288. Wild-type strains and SYP-14288-resistant mutants of R. solani were incubated on potato dextrose agar amended with either SYP-14288 or one of select fungicides acting on fungal respiration, including complex I, II, and III inhibitors; uncouplers; and ATP synthase inhibitors. Mycelial growth was measured under fungicides treatments. ATP content was determined using an ATP assay kit, membrane potential of mitochondria was detected with the JC-1 kit, and respiratory rate was calculated based on the measurement of oxygen consumption of R. solani. A model of metabolic fingerprinting cluster was established to separate oxidation inhibitors and phosphorylation inhibitors. All the results together displayed a clear discrimination between oxidation inhibitors and phosphorylation inhibitors, and the latter inhibited ATP synthase production having or uncoupling activities. Based on the model, SYP-14288 was placed in phosphorylation inhibitor group, because it significantly reduced ATP content and membrane potential of mitochondria while increasing respiratory rate in R. solani. Therefore, the MoA of SYP-14288 on R. solani was confirmed to involve phosphorylation inhibition and possibly uncoupling activity.

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Section: Introductionmentioning

confidence: 99%

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

et al. 2020

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“…Gibberella zeae ( G. zeae ) distributed all over the world is a devastating filamentous fungus. It can cause fusarium head blight disease in several economically important crops, such as maize, barley, and wheat [ 4 , 5 ]. How to prevent these diseases caused by plant pathogenic fungi is a thought-provoking question.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Antifungal Property of Hydroxypropyltrimethyl Ammonium Chitosan Halogenated Acetates

Tan

Zhang

et al. 2018

Marine Drugs

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Hydroxypropyltrimethyl ammonium chitosan halogenated acetates were successfully synthesized from six different haloacetic acids and hydroxypropyltrimethyl ammonium chloride chitosan (HACC) with high substitution degree, which are hydroxypropyltrimethyl ammonium chitosan bromacetate (HACBA), hydroxypropyltrimethyl ammonium chitosan chloroacetate (HACCA), hydroxypropyltrimethyl ammonium chitosan dichloroacetate (HACDCA), hydroxypropyltrimethyl ammonium chitosan trichloroacetate (HACTCA), hydroxypropyltrimethyl ammonium chitosan difluoroacetate (HACDFA), and hydroxypropyltrimethyl ammonium chitosan trifluoroacetate (HACTFA). These chitosan derivatives were synthesized by two steps: first, the hydroxypropyltrimethyl ammonium chloride chitosan was synthesized by chitosan and 3-chloro-2-hydroxypropyltrimethyl ammonium chloride. Then, hydroxypropyltrimethyl ammonium chitosan halogenated acetates were synthesized via ion exchange. The structures of chitosan derivatives were characterized by Fourier transform infrared spectroscopy (FTIR), 1H Nuclear magnetic resonance spectrometer (1H NMR), 13C Nuclear magnetic resonance spectrometer (13C NMR), and elemental analysis. Their antifungal activities against Colletotrichum lagenarium, Fusarium graminearum, Botrytis cinerea, and Phomopsis asparagi were investigated by hypha measurement in vitro. The results revealed that hydroxypropyltrimethyl ammonium chitosan halogenated acetates had better antifungal activities than chitosan and HACC. In particular, the inhibitory activity decreased in the order: HACTFA > HACDFA > HACTCA > HACDCA > HACCA > HACBA > HACC > chitosan, which was consistent with the electron-withdrawing property of different halogenated acetates. This experiment provides a potential idea for the preparation of new antifungal drugs by chitosan.

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“…All handling and bioassays were performed under aseptic conditions in a laminar flow cabinet following good laboratory practice (GLP) and standard operating procedures (SOP). The plugs were then placed in the center of sterile cellophane membranes (9-cm in diameter, 500 PUT; UCB, North Augusta, USA) in a Petri plate (9-cm in diameter) containing 25 mL of PDA amended or not with 2 mg L −1 , which is the WT׳ s sub-lethal concentration [1] , or 10 mg L −1 of carbendazim. The concentration of 10 mg L −1 carbendazim was only applied to FG1 and FG2 .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Fusarium graminearum 1H NMR metabolomics

et al. 2018

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Raw 1H NMR spectra of Fusarium graminearum hyphae can be found at the website of the pesticide metabolomics group (PMG) of the Agricultural University of Athens at the address: http://www.aua.gr/pesticide-metabolomicsgroup/Resources/Fusarium_graminearum_NMR_spectra.html, accession number PMG-01–17. The data set support the research article “Implication of Fusarium graminearum Primary Metabolism in its Resistance to Benzimidazole Fungicides as revealed by 1H NMR Metabolomics” [1].

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Implication of Fusarium graminearum primary metabolism in its resistance to benzimidazole fungicides as revealed by 1H NMR metabolomics

Cited by 36 publications

References 74 publications

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

Synthesis, Characterization, and Antifungal Property of Hydroxypropyltrimethyl Ammonium Chitosan Halogenated Acetates

Fusarium graminearum 1H NMR metabolomics

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