Implicaciones del factor de transcripción WT1 asociado al remodelado cardiaco patológico postinfarto miocárdico

Sanz, Raúl; Mazzei, Luciana; Manucha, Walter

doi:10.1016/j.arteri.2018.08.003

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Additionally, proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, could favor Wt1 activation after MI through activation of NF-κB. Furthermore, WT1 expression after injury might also be induced by soluble factors secreted by the myocardium [ 82 ]. This might induce progenitor cell proliferation and cell survival [ 83 , 84 ].…”

Section: Discussionmentioning

confidence: 99%

Implications of the Wilms’ Tumor Suppressor Wt1 in Cardiomyocyte Differentiation

Wagner

Ninkov

Vukolic

et al. 2021

IJMS

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The Wilms’ tumor suppressor Wt1 is involved in multiple developmental processes and adult tissue homeostasis. The first phenotypes recognized in Wt1 knockout mice were developmental cardiac and kidney defects. Wt1 expression in the heart has been described in epicardial, endothelial, smooth muscle cells, and fibroblasts. Expression of Wt1 in cardiomyocytes has been suggested but remained a controversial issue, as well as the role of Wt1 in cardiomyocyte development and regeneration after injury. We determined cardiac Wt1 expression during embryonic development, in the adult, and after cardiac injury by quantitative RT-PCR and immunohistochemistry. As in vitro model, phenotypic cardiomyocyte differentiation, i.e., the appearance of rhythmically beating clones from mouse embryonic stem cells (mESCs) and associated changes in gene expression were analyzed. We detected Wt1 in cardiomyocytes from embryonic day (E10.5), the first time point investigated, until adult age. Cardiac Wt1 mRNA levels decreased during embryonic development. In the adult, Wt1 was reactivated in cardiomyocytes 48 h and 3 weeks following myocardial infarction. Wt1 mRNA levels were increased in differentiating mESCs. Overexpression of Wt1(-KTS) and Wt1(+KTS) isoforms in ES cells reduced the fraction of phenotypically cardiomyocyte differentiated clones, which was preceded by a temporary increase in c-kit expression in Wt1(-KTS) transfected ES cell clones and induction of some cardiomyocyte markers. Taken together, Wt1 shows a dynamic expression pattern during cardiomyocyte differentiation and overexpression in ES cells reduces their phenotypical cardiomyocyte differentiation.

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Section: Discussionmentioning

confidence: 99%

Implications of the Wilms’ Tumor Suppressor Wt1 in Cardiomyocyte Differentiation

Wagner

Ninkov

Vukolic

et al. 2021

IJMS

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“…GPCR ligand binding [60], extracellular matrix organization [61], cytokine signaling in immune system [62], interferon signaling [63], signaling by GPCR [64], neuronal system [65] and platelet activation, signaling and aggregation [66] plays an important role in the schizophrenia. Studies have revealed that SIX1 [67], VIP (vasoactive intestinal peptide) [68], GATA6 [69], FRZB (frizzled related protein) [70], CD40 [71], WT1 [72], PCDHGA3 [73], TFAP2B [74], HFE (homeostatic iron regulator) [75], NKX2-5 [76], IGFBP7 [77], HLA-F [78], CCL2 [79], COL1A2 [80], RUNX1 [81], TFF3 [82], IRX4 [83], NOS1 [84], DKK2 [85], IL18R1 [86], ADAM12 [87], NPPC (natriuretic peptide C) [88], COL1A1 [89], ABCG2 [90], SIX2 [91], CSRP1 [92], MR1 [93], NINJ2 [94], ACE (angiotensin I converting enzyme) [95], TBX1 [96], CTSC (cathepsin C) [97], DLX6 [98], KCNE1 [99], AZGP1 [100], CYP1B1 [101], PRRX1 [102], CD34 [103], A2M [104], CDKN2A [105], SERPINE1 [106], CD44 [107], FABP4 [108], ITGB3 [109], ALOX5AP [110], DAND5 [111], SFRP4 [112], RUNX2 [113], TACR3 [114], MYD88 [115], CYBA (cytochrome b-245 alpha chain) [116], STAT6 [117], FOXC1 [118], FN1 [119], TLR6 [120], CAV1 [121], RGS4 [122], TPM2 [123], TNFSF4 [124], LOX (lysyl oxidase) [125], SMOC2 [126], SPHK1 [127], FOLH1 [128], CYP2C8 [129], CD163 [130], DIRAS3 [131], OSMR (oncostatin M receptor) [132], POSTN (peri...…”

Section: Discussionmentioning

confidence: 99%

Screening of the Key Genes and Signaling Pathways for Schizophrenia Using Bioinformatics and Next Generation Sequencing Data Analysis

Vastrad,

Vastrad

2023

Preprint

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Schizophrenia is thought to be the most prevalent chronic psychiatric disorder. Numerous proteins have been identified that are associated with the occurrence and development of schizophrenia. This study aimed to identify potential core genes and pathways involved in schizophrenia, through exhaustive bioinformatic and next generation sequencing (NGS) data analyses using GSE20966 NGS data of neural progenitor cells and neurons obtained from healthy controls and patients with schizophrenia. The NGS data were downloaded from the Gene Expression Omnibus database. NGS data was processed by the DESeq2 package in R software and the differentially expressed genes (DEGs) were identified. Gene Ontology (GO) enrichment analysis and REACTOME pathway enrichment analysis were carried out to identify potential biological functions and pathways of the DEGs. Protein-protein interaction (PPI) network, module, miRNA-hub gene regulatory network and TF-hub gene regulatory network analysis were performed to identify the hub genes, miRNA and TFs. Potential hub genes were analyzed using receiver operating characteristic (ROC) curves in the R package (pROC). In this investigation, an overall 955 DEGs were identified: 478 genes were remarkably up regulated and 477 genes were distinctly down regulated. These genes were enriched for GO terms and pathways mainly involved in the multicellular organismal process, GPCR ligand binding, regulation of cellular process and amine ligand-binding receptors. MYC, FN1, CDKN2A, EEF1G, CAV1, ONECUT1, SYK, MAPK13, TFAP2A and BTK were considered the potential hub genes. miRNA-hub gene regulatory network and TF-hub gene regulatory network were constructed successfully. On the whole, the findings of this investigation enhance our understanding of the potential molecular mechanisms of schizophrenia and provide potential targets for further investigation.

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Genomic or Non-Genomic? A Question about the Pleiotropic Roles of Vitamin D in Inflammatory-Based Diseases

et al. 2023

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Vitamin D (vit D) is widely known for its role in calcium metabolism and its importance for the bone system. However, various studies have revealed a myriad of extra-skeletal functions, including cell differentiation and proliferation, antibacterial, antioxidant, immunomodulatory, and anti-inflammatory properties in various cells and tissues. Vit D mediates its function via regulation of gene expression by binding to its receptor (VDR) which is expressed in almost all cells within the body. This review summarizes the pleiotropic effects of vit D, emphasizing its anti-inflammatory effect on different organ systems. It also provides a comprehensive overview of the genetic and epigenetic effects of vit D and VDR on the expression of genes pertaining to immunity and anti-inflammation. We speculate that in the context of inflammation, vit D and its receptor VDR might fulfill their roles as gene regulators through not only direct gene regulation but also through epigenetic mechanisms.

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Implicaciones del factor de transcripción WT1 asociado al remodelado cardiaco patológico postinfarto miocárdico

Cited by 3 publications

References 33 publications

Implications of the Wilms’ Tumor Suppressor Wt1 in Cardiomyocyte Differentiation

Implications of the Wilms’ Tumor Suppressor Wt1 in Cardiomyocyte Differentiation

Screening of the Key Genes and Signaling Pathways for Schizophrenia Using Bioinformatics and Next Generation Sequencing Data Analysis

Genomic or Non-Genomic? A Question about the Pleiotropic Roles of Vitamin D in Inflammatory-Based Diseases

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