Implementation of Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System

Kan, Hyo; Yamada, Kento; Sanada, Nobuyuki; Nakata, Koyo; Tsukagoshi, Kazuhiko

doi:10.2116/analsci.34.239

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…Tsukagosi et al reported the phenomenon named tube radial distribution phenomenon (TRDP) [17][18][19]. In TRDP, the ternary mixture of solvents in the capillary results in a composition distribution in the radial direction, i.e., the solvent composition at the center of the tube is different from that at the surface.…”

Section: Study Of Analytical Stabilitymentioning

confidence: 99%

Electrophoretic Behavior in Nonaqueous Capillary Zone Electrophoresis Using Tetrahydrofuran-Based Ternary Nonaqueous Solutions

et al. 2018

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Nonaqueous capillary electrophoresis has the potential to separate water-insoluble analytes. Previously, we reported the separation of water-insoluble and neutral synthetic polymers in nonaqueous capillary zone electrophoresis using tetrahydrofuran (THF)-based ternary nonaqueous solutions. In this paper, the effect of the composition of a THF-based ternary solution (THF, acetonitrile, and methanol) containing cetyltrimethylammonium chloride (CTAC) on electrophoretic behavior is investigated using small organic compounds as prove analytes. In addition to general electrophoretic parameters such as electrophoretic mobility, analytical stability was also evaluated. The electric current, electrophoretic mobility of the benzyltriethylammonium cation (ion pair formation with chloride anion), and electrophoretic mobility of coronene (solvophobic interaction with the CTA cation) were dominated mainly by the THF content, and lower THF content resulted in higher values. In contrast, electroosmotic mobility was enhanced by an increase in THF content and was affected mainly by methanol content. Interestingly, in addition to stable electropherograms, we observed instable (fluctuation of baseline or zero current) electropherograms, which were obtained irregularly. Although a notable relation between the composition and the instable behavior was not observed, it is suggested that this "discontinuous instability" is related with the composition distribution (heterogeneous composition) of the ternary solvents in a capillary. Increasing the temperature effectively suppressed the composition distribution and has the potential to decrease the risk of instable behavior.

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Section: Study Of Analytical Stabilitymentioning

confidence: 99%

Electrophoretic Behavior in Nonaqueous Capillary Zone Electrophoresis Using Tetrahydrofuran-Based Ternary Nonaqueous Solutions

et al. 2018

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“…[13][14][15] The outer phase in TRDF acts as a pseudo-stationary phase under laminar flow conditions during chromatographic separation. Recently we also studied TRDC from the viewpoint of the separation tube materials 16 as well as pressure loss on the separation performance. 17 In 2019 Lam et al offered a critical review in The Analyst under the title "Recent advances in open-tubular capillary liquid chromatography".…”

Section: Introductionmentioning

confidence: 99%

Consecutive Sample Injection Analysis in Tube Radial Distribution Chromatography

et al. 2021

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Tube radial distribution chromatography based on the tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported, where the annular flow is created through phase-separation multiphase flow. We have proposed the first-ever procedure for consecutive sample injection analysis using chromatography. In basic terms, a commercially available HPLC system could be used with a sample injector (0.2 μL volume) and a fused-silica capillary tube (250 cm long) as a separation column instead of a normal packed one, while the built-in detection cell was replaced by improved on-capillary detection. A ternary mixed solution of water/acetonitrile/ethyl acetate (3:8:4 volume ratio) was delivered into the capillary tube as an eluent at a flow rate of 2.0 μL min -1 . Model sample solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid were consecutively analyzed by the present chromatography with a processing rate of 6 samples per hour. Simple and rapid consecutive analysis could be performed because washing and initialization of the separation tube was no longer necessary. The obtained results provide clues to developing new methodologies which combine features of both chromatography (separation) and the flow injection method (consecutive analysis).

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“…Novel capillary chromatography has been performed based on the specific TRDF; we call the separation method tube radial distribution chromatography (TRDC). [10][11][12] In the TRDC, the outer phase acts as a pseudo-stationary phase during chromatographic separation.…”

Section: Introductionmentioning

confidence: 99%

Development of Tube Radial Distribution Chromatography Based on Phase-Separation Multiphase Flow Created via Pressure Loss

et al. 2019

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A tube radial distribution chromatography (TRDC) method based on phase-separated multiphase flow created through phase transformation via temperature change has been developed. These systems typically required a temperaturecontrolling device containing a water bath and a stirrer. Herein, we proposed a novel TRDC system without a cooling device, where the phase transformation was achieved via pressure loss in a capillary tube of 50 μm inner diameter and 550 cm length. Model analytes were successfully separated with the developed TRDC system, which provided a simplified platform and helped to clarify the operating principle of TRDC based on phase transformation in a capillary tube.

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Implementation of Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System

Cited by 11 publications

References 6 publications

Electrophoretic Behavior in Nonaqueous Capillary Zone Electrophoresis Using Tetrahydrofuran-Based Ternary Nonaqueous Solutions

Electrophoretic Behavior in Nonaqueous Capillary Zone Electrophoresis Using Tetrahydrofuran-Based Ternary Nonaqueous Solutions

Consecutive Sample Injection Analysis in Tube Radial Distribution Chromatography

Development of Tube Radial Distribution Chromatography Based on Phase-Separation Multiphase Flow Created via Pressure Loss

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