Implementation of routine thromboplastin-plasma cell block technique in the evaluation of non-gynecologic specimens: A methodologic comparison with conventional cytology

Castro-Villabón, Diana; Avello, Yubelly; Ruiz, Nathalie Soriano; Rodríguez‐Urrego, Paula A.

doi:10.1016/j.jmau.2014.05.001

Cited by 5 publications

(2 citation statements)

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“…The sample is replenished in 0.1 mL of pooled plasma, followed by 0.2 mL of reconstituted thrombin at room temperature, and is mixed well quickly. [ 27 - 29 ]…”

Section: Cell-blocking Methodsmentioning

confidence: 99%

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Contemporary art of cell-block preparation: Overview

Saharti

2024

Cytojournal

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Cell blocks (CBs) are paraffin-embedded versions of cytology specimens. These versions are contrasted with tissues made from surgical pathology specimens of formalin-fixed paraffin-embedded (FFPE) tissue. CBs enable various elective ancillary studies of a range of specimens. These studies include the potential to perform molecular tests with the enhanced cytopathological interpretation. CBs are increasingly reported in cytology specimens. The enhanced role of CBs incorporates additives with new markers for immunohistochemistry (IHC), including the multicolored approach to IHC, and the subtractive coordinate immunoreactivity pattern. Even when archived material is retrospectively retrieved, CBs are a major tissue source for many supplementary studies. The CBs have been qualitatively and quantitatively improved. CBs are significant since they have increased molecular markers standardized on FFPE tissue. High-quality CBs can serve as useful additions to cytological smear preparations and touch imprint cytology. Most cytological specimens, such as fine-needle aspirations, cavitary effusion, washings, brushings, and gynecological and non-gynecological liquid specimens, may be used to produce CBs. This review deals with the CB-making process and discusses various historical limitations with an emphasis on recent advances.

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“…The sample is replenished in 0.1 mL of pooled plasma, followed by 0.2 mL of reconstituted thrombin at room temperature, and is mixed well quickly. [ 27 - 29 ]…”

Section: Cell-blocking Methodsmentioning

confidence: 99%

“…When no clot forms after 5 min, an extra 0.2 mL of thrombin may be added. [ 29 ] The coat is then slid over and wrapped in a piece of filter paper. The pellet is finally embedded in paraffin after being fixed in 10% neutral buffered formalin (NBF).…”

Section: Cell-blocking Methodsmentioning

confidence: 99%