Implementation of HPLC Analysis for γ-Aminobutyric Acid (GABA) in Fermented Food Matrices

Le, Phuong Hong; Verscheure, Liesa; Le, Thien Trung; Verheust, Yannick

doi:10.1007/s12161-020-01734-2

Cited by 26 publications

(11 citation statements)

References 39 publications

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“…In raw rice, GABA content was found to be 1.61 ± 0.001 μg/mL, whereas in L. reuterii fermented brown rice it was 27.03 ± 0.055 μg/mL. In this research, we found a higher GABA concentration than reported by Cáceres et al and Le et al [ 27 , 28 ].…”

Section: Resultscontrasting

confidence: 50%

Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

et al. 2021

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Free radical-induced oxidative stress is the root cause of many diseases, such as diabetes, stress and cardiovascular diseases. The objective of this research was to screen GABA levels, antioxidant activities and bioactive compounds in brown rice. In this study, we first fermented brown rice with different lactic acid bacteria (LABs), and the best LAB was selected based on the levels of GABA in the fermentate. Lactobacillus reuterii generated the highest levels of GABA after fermentation. To ascertain whether germination can improve the GABA levels of brown rice, we compared the levels of GABA in raw brown rice (Raw), germinated brown rice (Germ), fermented brown rice (Ferm) and fermented-germinated brown rice (G+F) to identify the best approach. Then, antioxidant activities were investigated for Raw BR, Germ BR, Ferm BR and G+F BR. Antioxidant activity was calculated using a 2,2-diphenyl-1-picryl hydrazile radical assay, 2,2-azino-bis-(3-ethylene benzothiozoline-6-sulfonic acid) radical assay and ferric-reducing antioxidant power. In Ferm BR, DPPH (114.40 ± 0.66), ABTS (130.52 ± 0.97) and FRAP (111.16 ± 1.83) mg Trolox equivalent 100g, dry weight (DW), were observed as the highest among all samples. Total phenolic content (97.13 ± 0.59) and total flavonoids contents (79.62 ± 1.33) mg GAE/100 g and catechin equivalent/100 g, DW, were also found to be highest in fermented BR. Furthermore, an untargeted metabolomics approach using ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of bioactive compounds in fermented BR, such as GABA, tryptophan, coumaric acid, L-ascorbic acid, linoleic acid, β-carotenol, eugenol, 6-gingerol, etc., as well as bioactive peptides which could contribute to the health-promoting properties of L. reuterii fermented brown rice.

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Section: Resultscontrasting

confidence: 50%

Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

et al. 2021

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“…Overall, the recoveries of amino acids 89.9–117.5% with the RSDs less than 9.7% ( n = 3) are detected. Furthermore, for reference, the same samples were also analyzed by conventional HPLC [39]. The low relative error of less than –6.7% in Table 2 demonstrated that there is no significant difference between these two methods.…”

Section: Resultsmentioning

confidence: 99%

An integrated plastic microchip for enhancing electrophoretic separation using tunable pressure‐driven backflows

et al. 2022

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Microfluidic CE (MCE) is an effective solution for rapid and sensitive determination of multiple analytes. Herein, a dynamic coated cyclic olefin copolymer microchip was developed having an on‐chip micropump for fluid velocity adjusting in electrophoretic separations. This micropump was fabricated by constructing a polyacrylamide gel membrane at one channel terminal. Once applying electric field across the membrane, a pressure‐driven flow generated automatically to balance the electroosmotic flow (EOF) mismatch at the channel‐membrane interface. The influence of gel precursor concentration and operating voltages on the fluid velocity was carefully evaluated. Moreover, the highly integration of injection, separation, and pumping units of the MCE system minimized the dead volume and provides satisfied column efficiency. Experiments showed that by adjusting of pumping voltage reduced the fluid velocity by a factor of 6, resulting six‐ and threefold resolving power enhancements of rhodamine dye mixture and amino acid mixture, respectively. Furthermore, the developed MCE method was applied for rhodamines and amino acids quantitation in food and cosmetics, with standard addition recoveries of 87.3–106.9% and 89.9–117.4%, respectively. These results were also confirmed by standard HPLC method, revealing the application potential in fast and onsite analysis of complex samples.

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“…o-Phthalaldehyde (OPA) reacts with primary amines in the presence of 2-mercaptoethanol (MCE) generating a reaction product (a carboxylic acid) whose chromophore groups allow its maximum detection at 338 nm. [18][19][20][21] Scheme S2 illustrates amino acid derivatization with OPA-MCE (supporting information, section 3.15, p. S13).…”

Section: Enzymatic Immobilizationmentioning

confidence: 99%

α‐Ketobutyrate Production under Continuous‐Flow Conditions Catalyzed by Immobilized L‐Methionine γ‐Lyase

et al. 2022

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l-Methionine γ-lyase (MGL) is a PLP-dependent enzyme which catalyzes the α, γ-elimination reaction in sulfur containing amino acids, such as L-methionine and L-cysteine. Its major applications are on metabolic restriction of methionine to cancer cells, food industry (cheese flavor) and for the synthesis of d and l-homoalanine. Herein, we present the immobilization and use of this new immobilized biocatalyst under batch and continuous-flow conditions. The best optimized continuousflow condition allowed 89 % conversion in 30 min of residence time with good recyclability of the biocatalyst.

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Implementation of HPLC Analysis for γ-Aminobutyric Acid (GABA) in Fermented Food Matrices

Cited by 26 publications

References 39 publications

Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

An integrated plastic microchip for enhancing electrophoretic separation using tunable pressure‐driven backflows

α‐Ketobutyrate Production under Continuous‐Flow Conditions Catalyzed by Immobilized L‐Methionine γ‐Lyase

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