Implementation of Functional State Approach for Modelling ofEscherichia ColiFed-Batch Cultivation

Abstract

This paper presents the implementation of functional state approach to modelling of Escherichia coli fed-batch cultivation. Due to the complex metabolic pathways of microorganisms, the accurate modelling of bioprocesses is rather difficult. The functional state approach of a process is an alternative concept which helps in modelling and control of complex processes. The approach main idea is developing of models based on multiple submodels for each functional states (operating regime). In each functional state the process is described by a conventional type of model, called the local model, which is valid in this state. For parameter identification of the model the genetic algorithms are used. Genetic algorithms are directed random search techniques, applying the mechanics of natural selection and natural genetics, which can find the global optimal solution in complex multidimensional search spaces. Based on the available experimental data and simulations of E. coli fed-batch cultivation it is shown how this process can be divided into functional states and how the model parameters can be obtained on the basis of genetic algorithms. By simulation and comparison between the results and experimental data, can be seen how the concept of functional state approach works and how effective is the proposed identification scheme.

PID Controller Tuning based on Metaheuristic Algorithms for Bioprocess Control

“…the cultivation process was carried out in the institute of technical chemistry, hannover University, Germany. the cultivation conditions and data measurements are previously discussed (2,36). the model is presented as:…”

“…the GA operators and parameters are summarized in table 1 and table 2. these are the most appropriate GA parameters and operators for the problem considered here based on our earlier research (32,33,34,35) Annealing function…”

Metaheuristic Techniques for Optimization of anE. ColiCultivation Model

“…Process description the cultivation was performed in the institute of technical chemistry, University of hannover, Germany, during the collaboration work with the institute of Biophysics and Biomedical engineering, BAS, Bulgaria, granted by DFG (3,38). the strain used for the fermentation process was E. coli Mc4110.…”

Functional state modelling approach validation for yeast and bacteria cultivations

“…[ 11 ] Based on [ 12 ] it is demonstrated that there is an analogy between the S. cerevisiae and E. coli growth curves. [ 5 , 11 ]…”

“…Another common model microorganism is E. coli , whose cultivation is, in many cases, the only economically viable way to produce many biopharmaceuticals. [ 5 ] Since 1997, when the entire E. coli genome was published, scientific research on this bacterium has accelerated. [ 13 ]…”