This study purpose to develop an electronic module (e-module) based on project-based learning integrated with STEM (Science, Technology, Engineering, Mathematics) approach. This study is a research and development research, by adopting steps according to the ADDIE model, namely Analyze, Design, Develop, Implement and Evaluation. The data collection method used is using questionnaires and interviews, while the data analysis technique uses descriptive qualitative and quantitative analysis. The e-module developed is considered highly valid by material expert validators and media expert with score 95,83 and 94,23 respectively. The e-module practicality test was conducted on 30 students with score 98,67 or very good category. Based on the results of this study, it can be concluded that the STEM integrated project-based biotechnology e-module is declared very feasible and practical to be used in learning Biotechnology courses.