Implanted Nanosensors in Marine Organisms for Physiological Biologging: Design, Feasibility, and Species Variability

Lee, Michael A.; Nguyen, Freddy T.; Scott, Kathleen; Chan, Nathan Y.L.; Bakh, N.A.; Jones, Kelvin K.; Pham, Crystal; Garcia-Salinas, Pablo; García-Párraga, Daniel; Fahlman, Andreas; Marco, V.; Koman, Volodymyr B.; Oliver, Ronald J.; Hopkins, Lloyd W.; Rubio, Consuelo; Wilson, Rory P.; Meekan, Mark G.; Duarte, Carlos M.; Strano, Michael S.

doi:10.1021/acssensors.8b00538

Cited by 41 publications

(38 citation statements)

References 79 publications

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“…As the sensitivity of all nanosensor optical devices is strongly linked to the brightness of the emitting fluorophore 24 , this creates problems for in vivo sensing applications. Furthermore, this limits the practical application of the sensors by reducing the penetration depth of the fluorescence emission 25,26 . While there are advantages to using alternative wrappings, like surfactants, to increase the quantum yields of nanotube sensors 23 , ultimately any wrapping replacing DNA sacrifices the increased biocompatibility and vast combinatorial library available for this biopolymer.…”

Section: Introductionmentioning

confidence: 99%

Modulating the properties of DNA-SWCNT sensors using chemically modified DNA

Gillen

Lambert

Antonucci

et al. 2021

Preprint

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Properties of SWCNT-based sensors such as brightness and detection capabilities strongly depend on the characteristics of the wrapping used to suspend the nanotubes. In this study, we explore ways to modify the properties of DNA-SWCNT sensors by using chemically modified DNA sequences, with the aim of creating sensors more suitable for use in in vivo and in vitro applications. We show that both the fluorescence intensity and sensor reactivity are strongly impacted not only by the chemical modification of the DNA but also by the method of preparation. In the absence of modifications, the sensors prepared using MeOH-assisted surfactant exchange exhibited higher overall fluorescence compared to those prepared by direct sonication. However, we demonstrate that the incorporation of chemical modifications in the DNA sequence could be used to enhance the fluorescence intensity of sonicated samples. We attribute these improvements to both a change in dispersion efficiency as well as to a change in SWCNT chirality distribution. Furthermore, despite their higher intensities, the response capabilities of sensors prepared by MeOH-assisted surfactant exchange were shown to be significantly reduced compared to their sonicated counterparts. Sonicated sensors exhibited a globally higher turn-on response towards dopamine compared to the exchanged samples, with modified samples retaining their relative intensity enhancement. As the increases in fluorescence intensity were achieved without needing to alter the base sequence of the DNA wrap- ping or to add any exogenous compounds, these modifications can - in theory - be applied to nearly any DNA sequence to increase the brightness and penetration depths of a variety of DNA-SWCNT sensors without affecting biocompatibility or reducing the near-limitless sequence space available. This makes these sensors an attractive alternative for dopamine sensing in vitro and in vivo by enabling significantly higher penetration depths and shorter laser exposure times.

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Section: Introductionmentioning

confidence: 99%

Modulating the properties of DNA-SWCNT sensors using chemically modified DNA

Gillen

Lambert

Antonucci

et al. 2021

Preprint

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“…Despite not reacting to the Ca 2+ cations, the (7,6) peak fluorescence intensity was significantly enhanced by the addition of dopamine. In fact, the final intensity of the LNA every6G -SWCNTs was higher (normalized to sensor concentration) than the (GT) 15 -SWCNTs, implying that these sensors would additionally benefit from increased penetration depth in biological samples 12,32 . Furthermore, the distinct responses of the (7,5) and (7,6) peaks to CaCl 2 and dopamine enabled simultaneous multi-modal sensing of these two analytes.…”

Section: Resultsmentioning

confidence: 99%

Distinguishing dopamine and calcium responses using XNA-nanotube sensors for improved neurochemical sensing

Gillen

Antonucci

Reggente

et al. 2021

Preprint

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To date, the engineering of single-stranded DNA-SWCNT (DNA-SWCNT) optical biosensors have largely focused on creating sensors for new applications with little focus on optimising existing sensors for in vitro and in vivo conditions. Recent studies have shown that nanotube fluorescence can be severely impacted by changes in local cation concentrations. This is particularly problematic for neurotransmitter sensing applications as spatial and temporal fluctuations in the concentration of cations, such as Na+, K+, or Ca2+, play a central role in neuromodulation. This can lead to inaccuracies in the determination of neurotransmitter concentrations using DNA-SWCNT sensors, which limits their use for detecting and treating neurological diseases. Herein, we present new approaches using locked nucleic acid (LNA) to engineer SWCNT sensors with improved stability towards cation-induced fluorescence changes. By incorporating LNA bases into the (GT)15-DNA sequence, we create sensors that are not only more resistant towards undesirable fluorescence modulation in the presence of Ca2+ but that also retain their capabilities for the label-free detection of dopamine. The synthetic biology approach presented in this work therefore serves as a complementary means for enhancing nanotube optoelectronic behavior, unlocking previously unexplored possibilities for developing nano-bioengineered sensors with augmented capabilities.

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“…By carrying out a study of references and scientific proposals on SGs, we can retrieve thousands of sources, most of them related to Smart Power Grids, Power Generation Systems, Electric Power Systems, Economy and Management of the Electric Network, and Control and Optimization of Electric Systems [18,19]. However, a list of SGs with sensors applied to the home limits the list of sources quite a bit.…”

Section: Related Researchmentioning

confidence: 99%

Smart Grids and Their Role in Transforming Human Activities—A Systematic Literature Review

2020

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In this work, a systematic review of the literature has been carried out to analyse the design of intelligent networks in environments inhabited by people and the applications of sensors to improve quality of life and aid human activities. This study aims to answer three research questions. The first question is whether the design of smart grids is made with people in mind. The second question focuses on whether intelligent networks are being taken account of in the research on human activity recognition, the Internet of Things, and the recognition of activities of daily living. The third question looks at whether there are synergies and multidisciplinary teams studying state-of-the-art technologies applied to environments inhabited by elderly or disabled people. Installations with sensors deployed for the improvement of the quality of human life will also help to improve the quality of the intelligent network, thus integrating the Human–Technology binomial. This study concludes with an analysis of the results of the sources examined, putting forward a protocol of seven proposals to guide future work.

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Implanted Nanosensors in Marine Organisms for Physiological Biologging: Design, Feasibility, and Species Variability

Cited by 41 publications

References 79 publications

Modulating the properties of DNA-SWCNT sensors using chemically modified DNA

Modulating the properties of DNA-SWCNT sensors using chemically modified DNA

Distinguishing dopamine and calcium responses using XNA-nanotube sensors for improved neurochemical sensing

Smart Grids and Their Role in Transforming Human Activities—A Systematic Literature Review

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