Implantation study of a tissue-engineered self-expanding aortic stent graft (bio stent graft) in a beagle model

Abstract

The use of stent grafts for endovascular aortic repair has become an important treatment option for aortic aneurysms requiring surgery. This treatment has achieved excellent outcomes; however, problems like type 1 endoleaks and stent graft migration remain. Bio stent grafts (BSGs), which are self-expanding stents covered with connective tissue, were previously developed using "in-body tissue architecture" technology. We assessed their early adaptation to the aorta after transcatheter implantation in a beagle model. BSGs were prepared by subcutaneous embedding of acryl rods mounted with self-expanding nitinol stents in three beagles for 4 weeks (n = 3/dog). The BSGs were implanted as allografts into infrarenal abdominal aortas via the femoral artery of three other beagles. After 1 month of implantation, aortography revealed no stenosis or aneurysmal changes. The luminal surface of the BSGs was completely covered with neointimal tissue, including endothelialization, without any thrombus formation. The cover tissue could fuse the luminal surface of the native aorta with tight conjunctions even at both ends of the stents, resulting in complete impregnation of the strut into the reconstructed vascular wall, which is expected to prevent endoleaks and migration in clinical applications.

Modulation of cell function by electric field: a high-resolution analysis

“…Indeed, in our previous studies [3], we discovered that high-frequency EF stimulation results in activation of vascular endothelial cells and angiogenic cell responses via the mechanism consistent with the model presented above. The results of the present study, therefore, may lead to the development of new approaches for vascular stents, where endothelial cell activation, proliferation and migration can be the key factor that determines success or failure of the stent [79,80].…”

Three-Month Outcomes of Aortic Valve Reconstruction Using Collagenous Membranes (Biosheets) Produced by in Body Tissue Architecture in A Goat Model: A Preliminary Study

“…Some papers have reported that biosheets based on iBTA can be reconstructed in a manner analogous to that of native tissue, for example, as cornea, trachea (biosheet), and blood vessels (biotube) [8,9]. The technology based on iBTA have also been applied to heart valves (biovalve and stent-biovalve) [10,11] and stent grafts (bio stent graft) [12]. The papers that reported on this technology indicated the possibility of tissue regeneration, self-repair, and growth adaptability.…”

The Technological Advancement to Engineer Next‐Generation Stent‐Grafts: Design, Material, and Fabrication Techniques

“…BioSGs are composed of a metallic stent sandwiched or attached to a TEVG, aimed to improve postoperative outcomes (e.g., no risk of graft infection or aortoenteric fistula developing, and antithrombogenicity from early endothelialization). [99][100][101] To enhance the overall sealing performance, a unique graft system was developed [99] by compounding degradable polyglycolide (PGA, core) and nondegradable PET (sheath) fibers (Figure 5a). This configuration encouraged tissue ingrowth from the aortic wall into the graft fabric, where late migration and endoleak postdeployment were effectively avoided.…”

“…BioSGs are composed of a metallic stent sandwiched or attached to a TEVG, aimed to improve postoperative outcomes (e.g., no risk of graft infection or aortoenteric fistula developing, and anti‐thrombogenicity from early endothelialization). [ 99–101 ]…”

“…[ 100 ] Copyright 2015, Springer Nature; c) intraluminal surface of the graft after 30 d of BioSG implantation was flat with no thrombosis, and the BioSG wall was tightly integrated within the native aorta circumferentially. [ 101 ] Reproduced with permission. [ 101 ] Copyright 2015, John Wiley & Sons, Inc.…”