Implantation Rates after Two, Three, or Five Days of Embryo Culture

Santos, Ma José de los; Mercader, Amparo; Galán, Arancha; Albert, Carmela; Romero, Josep Lluís; Pellicer, A.

doi:10.1016/s0143-4004(03)00172-3

Cited by 54 publications

(43 citation statements)

References 53 publications

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“…The authors mentioned that if culture conditions are optimized, no differences would be seen in pregnancy rate after ET on day 1or day 2. In other studies, embryos transferred on day 2 were comparable to day 3 (5,6,(17)(18)(19)(20). Although in these studies, in patients with good prognosis, fewer embryos were transferred, in our survey, between the two groups, the number of embryos transferred remained unchanged.…”

Section: Discussionsupporting

confidence: 43%

A Comparative Analysis Between Day 2 and Day 3 Embryo Transfer in IVF/ICSI: A Retrospective Cross-Sectional Study

Modares¹,

Zamaniyan²,

Baheiraei³

et al. 2016

IJWHR

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Section: Discussionsupporting

confidence: 43%

A Comparative Analysis Between Day 2 and Day 3 Embryo Transfer in IVF/ICSI: A Retrospective Cross-Sectional Study

Modares¹,

Zamaniyan²,

Baheiraei³

et al. 2016

IJWHR

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“…These findings suggest that attachment 1 is a phase in which components of the apical glycocalyx may mediate weak binding to receptors on trophectoderm (Aplin 2000, Kimber & Spanswick 2000. Less than half of the transferred embryos progressed from this stage to stable (shear stress-resistant) attachment, a figure that echoes the high rates of implantation failure in ART programmes (de los Santos et al 2003, Kovalevsky & Patrizio 2005. Staining at this stage with antibody to the apical glycoprotein CD164 showed no perceptible disturbance to distribution, indicating that interaction with the embryo may not yet have triggered a major reorganisation of the glycocalyx.…”

Section: Discussionmentioning

confidence: 90%

“…In both humans and mice, the apposition and adhesion stages are of short duration (Psychoyos 1986, Bergh & Navot 1992, Lopata 1996, Aplin 2000, and both observational and experimental studies have been limited by this constraint. Despite advances in assisted reproductive technology (ART), only 20-25% of the treatment cycles result in a live birth (de los Santos et al 2003). Unravelling the signals that pass between embryo and maternal cells will suggest strategies to reduce implantation failure and increase clinical pregnancy rates in women undergoing ART.…”

Section: Introductionmentioning

confidence: 99%

Early stages of implantation as revealed by an in vitro model

Singh¹,

Nardo²,

Kimber³

et al. 2010

Reproduction

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Our limited understanding of the processes underlying steroid hormonal control of human endometrial receptivity is largely due to the lack of a relevant model system. To overcome scarcity of material, we have developed a model in which mouse embryos attach to human Ishikawa cells, which express functional steroid hormone receptors. Blastocysts flushed from day 4 pregnant superovulated mice were transferred to confluent Ishikawa cell monolayers. After 48 h of co-culture, 85% of the blastocysts had attached loosely, but only 40% attached stably to the epithelial cell surface. In contrast, 95% of the embryos attached stably to tissue culture plastic. Thus, weak attachment of a majority of the embryos was followed by stronger adhesion of a smaller proportion. Seventeen percent of the transferred blastocysts modified the epithelial cell surface with loss of MUC1 at the attachment site, extending variably to adjacent epithelial cells. Initially, stable attachment occurred without disruption to the integrity of the epithelial monolayer, but at later stages after the embryo had spread laterally, displacement of subjacent cells was observed. A modest increase in stable attachment, but no changes to MUC1 clearance, was observed after assisted hatching. After 24 h priming of Ishikawa cells by 17b-oestradiol (OE 2 ) followed by 72-h incubation with medroxyprogesterone acetate and OE 2 , stable attachment increased from 40 to 70%. Initial attachment is efficient either in the presence or in the absence of hormone; steroid treatment increased the incidence of stable attachment. Implantation failure is predicted to occur in this model when embryos fail to progress from initial to stable attachment.

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“…Implantation failure remains an unsolved problem in reproduction. Indeed, the average implantation rate in vitro fertilization (IVF) is about 25% (de los Santos et al, 2003). The main reason for the *Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of baicalin on lipopolysaccharide (LPS)-induced implantation failure and the uterine endometrium in mice

Zhao¹

2011

Afr. J. Pharm. Pharmacol.

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The objective of this study is to investigate the protective effects of baicalin and to elucidate its immunological modulation at maternal-fetal interface. Lipopolysaccharide (LPS) was given via intraperitoneal injection to induce embryo implantation failure in mice which received baicalin at days 0.5 to 3.5 of gestation. The IFN-/IL-10, nitric oxide synthase (NOS) activities of uterine and endometrial tissues in each group (n = 16) were detected by ELISA, respectively. The ratio of IFN-/IL-10 and NOS activities increased significantly in the uterus of LPS-induced mice implantation failure and endometrial cells of LPS interference. In the baicalin pretreated mice followed by LPS administration, the ratio of IFN-/IL-10 and NOS activities were normalized by the middle dose baicalin to the control level, significantly lower than LPS treatment group. The same results were received in vitro study of LPS interference endometrial cells. The results indicate that baicalin has protective effects through inhibiting excessive IFN-and NO production to modulate the immunological balance at maternal-fetal interface.

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Implantation Rates after Two, Three, or Five Days of Embryo Culture

Cited by 54 publications

References 53 publications

A Comparative Analysis Between Day 2 and Day 3 Embryo Transfer in IVF/ICSI: A Retrospective Cross-Sectional Study

A Comparative Analysis Between Day 2 and Day 3 Embryo Transfer in IVF/ICSI: A Retrospective Cross-Sectional Study

Early stages of implantation as revealed by an in vitro model

Protective effects of baicalin on lipopolysaccharide (LPS)-induced implantation failure and the uterine endometrium in mice

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