Implantation potential and fetal viability of mouse embryos cultured in media supplemented with platelet-activating factor

Ryan, John P.; Spinks, N. R.; O’Neill, Chris; Wales, R. G.

doi:10.1530/jrf.0.0890309

Cited by 61 publications

(35 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Groups of 10 blastocysts were surgically transferred to each uterine horn on Day 2.5 in pseudopregnant Quackenbush outbred recipient female mice, as previously described [21]. Nine days after embryo transfer, recipients were killed, and the numbers of implantation sites and viable fetuses were identified.…”

Section: Embryo Transfermentioning

confidence: 99%

Culture of Zygotes Increases p53 Expression in B6 Mouse Embryos, which Reduces Embryo Viability1

Li¹,

Chandrakanthan²,

Chami³

et al. 2007

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

The expression of TRP53 in blastocysts that had been cultured from the zygote stage in vitro for 90 h was compared with that in blastocysts collected from the uterus in C57BL6 (B6) and in F1 hybrid (B6CBF1) strain mice. In both strains, there was little TRP53 detected in blastocysts collected from the uterus. There was some increased expression in cultured embryos from B6CBF1 mice and marked increased expression in cultured B6 blastocysts. In cultured B6 embryos, there was obvious accumulation of TRP53 within the nuclear region of embryonic cells. Cultured B6 zygotes had significantly poorer rates of blastocyst formation and of capacity to undergo implantation or form viable fetuses than cultured zygotes from B6CBF1 mice or B6 blastocysts collected from the uterus. Trp53 À/À zygotes (B6 background) were significantly more likely to form blastocysts than sibling wild-type embryos, with Trp53 þ/À embryos having an intermediate level of viability (P , 0.01). On transfer of blastocysts to recipient females, Trp53 À/À blastocysts were more likely to form viable fetuses than wild-type or heterozygous sibling blastocysts when the embryos resulted from culture of zygotes (P , 0.001). This shift in viability did not occur when embryos were only subjected to 24 h of culture from the compacted embryo stage. Culture in vitro in the B6 strain caused a marked increase in the expression and nuclear accumulation of TRP53. This expression was a significant cause of the loss of viability that occurs on culture of zygotes from this strain in vitro.assisted reproductive technology, cell survival, early development, embryo, implantation, in vitro fertilization, Mdm2, TRP53, zygote

show abstract

Section: Embryo Transfermentioning

confidence: 99%

Culture of Zygotes Increases p53 Expression in B6 Mouse Embryos, which Reduces Embryo Viability1

Li¹,

Chandrakanthan²,

Chami³

et al. 2007

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…LIF and EGF increase production of proteinases by mouse blastocysts (Harvey et al 1995), indicating a paracrine signalling pathway from endometrium to blastocyst, preparing the blastocyst for subsequent uterine invasion. Treatment of mouse embryos with PAF increases blastocyst cell number and also significantly improves implantation after transfer to recipient mice (Ryan et al 1990b). Antagonists to PAF, and also to interleukin-1 (IL-1) receptor, block implantation in mice (Ryan et al …”

Section: Growth Factors and Preimplantation Developmentmentioning

confidence: 99%

Growth factor expression and function in the human and mouse preimplantation embryo

Hardy¹,

Spanos²

2002

Journal of Endocrinology

289

191

View full text Add to dashboard Cite

There is increasing evidence that even before implantation, human development is regulated by embryonically and maternally derived growth factors. Studies in other mammalian species have shown that growth factors and their receptors are expressed by the preimplantation embryo and the reproductive tract. Furthermore, a number of growth factors have been shown to affect rate of embryo development, the proportion of embryos developing to the blastocyst stage, blastocyst cell number, metabolism and apoptosis. Growth factor ligands and receptors are also expressed in human embryos and the maternal reproductive tract, and supplementation of culture medium with exogenous growth factors affects cell fate, development and metabolism of human embryos in vitro. Autocrine, paracrine and endocrine pathways that may operate within the embryo and between the embryo and the reproductive tract before implantation are proposed.

show abstract

“…It can act in an autocrine and/or paracrine manner to improve murine embryo development (Stoddart et al 1996, O'Neil 1997, especially at low embryo culture densities (O'Neill 1998). Supplementation of culture medium with the biologically active C16 isoform of PAF (Stoddart et al 2001) increases implantation rate (Ryan et al 1990a), placental mass (Ryan et al 1990a) and the viability of embryos for uterine transfer (O'Neill et al 1989). Furthermore, PAF can increase mitosis (Roberts et al 1993) and reduce apoptosis (O'Neill 1998), blastocyst cell number (Stoddart et al 1996, O'Neill 1997 and the oxidative metabolism of glucose and lactate (Ryan et al 1989(Ryan et al , 1990b.…”

Section: Introductionmentioning

confidence: 99%

The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos

Gopichandran

Leese²

2006

Reproduction

113

View full text Add to dashboard Cite

Bovine preimplantation embryos develop more successfully when cultured in groups, proibably because of the increased production of, and exposure to, embryotrophic autocrine and paracrine factors. Using a novel embryo culture technique, this study had two aims: 1. to determine the distance over which potential paracrine interactions affect bovine embryo development in terms of blastocyst and hatching rates, cell counts and carbohydrate metabolism; 2. to investigate the effect of platelet-activating factor (PAF) supplementation on bovine embryo development and metabolism. Groups of 16 presumptive zygotes were attached to the bottom of a culture dish by the cell adhesive Cell-Tak in a 4 3 4 equidistant array. The distance between individual embryos in each group was 0-689 mm. Optimal blastocyst formation rate occurred when embryos were cultured 165 mm apart compared with control non-attached zygotes (Kruskal-Wallis followed by Mann-Whitney U test post-hoc; P < 0.05). Increasing the distance between embryos resulted in a further decline in blastocyst rate, which reached zero at 540 mm apart. Blastocyst cell number, pyruvate/glucose uptake and lactate production decreased as the interembryo distance increased from 240 to 465 mm (P < 0.05). Supplementation with PAF during conventional group culture enhanced blastocyst cell number, hatching rates and the oxidative metabolism of pyruvate and glucose. The data indicate that the distance between individual bovine embryos in culture influences preimplantation development, in particular blastocyst formation, cell number and metabolism. It is suggested that diffusible paracrine/autocrine factors, such as PAF, are in part responsible for the regulation of early embryo development.

show abstract

Implantation potential and fetal viability of mouse embryos cultured in media supplemented with platelet-activating factor

Cited by 61 publications

References 10 publications

Culture of Zygotes Increases p53 Expression in B6 Mouse Embryos, which Reduces Embryo Viability1

Culture of Zygotes Increases p53 Expression in B6 Mouse Embryos, which Reduces Embryo Viability1

Growth factor expression and function in the human and mouse preimplantation embryo

The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos

Contact Info

Product

Resources

About