Implantation of p(HEMA)-collagen composite into bone

Smetana, Karel; Štol, M.; Korbelár, P; Novák, M.; Adam, M.

doi:10.1016/0142-9612(92)90033-k

Cited by 35 publications

(16 citation statements)

References 10 publications

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“…Poly(2‐hydroxyethyl methacrylate) (pHEMA), an FDA‐approved biocompatible polymer, has been widely utilized in a variety of biomedical applications such as contact lens, intraocular lens, drug delivery, dental, and orthopedic implants 1–7. The polymer, rich of hydroxyl groups, is considered as a low fouling material, which resists nonspecific protein adsorption and cell adhesion.…”

Section: Introductionmentioning

confidence: 99%

Dual‐functional electrospun poly(2‐hydroxyethyl methacrylate)

Zhang

Lalani

Cheng

et al. 2011

J Biomedical Materials Res

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Poly(2-hydroxyethyl methacrylate) (pHEMA) has been widely used in many biomedical applications due to its well-known biocompatibility. For tissue engineering applications, porous scaffolds that mimic fibrous structures of natural extracellular matrix and possess high surface-area-to-volume ratios are highly desirable. So far, a systematic approach to control diameter and morphology of pHEMA fibers has not been reported and potential applications of pHEMA fibers have barely been explored. In this work, pHEMA was synthesized and processed into fibrous scaffolds using an electrospinning approach. Fiber diameters from 270 nm to 3.6 μm were achieved by controlling polymer solution concentration and electrospinning flow rate. Post-electrospinning thermal treatment significantly improves integrity of the electrospun membranes in water. The pHEMA microfibrous membranes exhibited water absorption up to 280% (w/w), whereas the pHEMA hydrogel only absorbed 70% water. Fibrinogen adsorption experiments demonstrate that the electrospun pHEMA fibers highly resist nonspecific protein adsorption. Hydroxyl groups on electrospun pHEMA fibers were further activated for protein immobilization. A bovine serum albumin (BSA) binding capacity as high as 120 mg BSA/g membrane was realized at an intermediate fiber diameter. The pHEMA fibrous scaffolds functionalized with collagen I significantly promoted fibroblast adhesion, spreading, and proliferation. We conclude that the electrospun pHEMA fibers are dual functional, that is, they resist nonspecific protein adsorption meanwhile abundant hydroxyl groups on fibers allow effective conjugation of biomolecules in a nonfouling background. High water absorption and dual functionality of the electrospun pHEMA fibers may lead to a number of potential applications such as wound dressings, tissue scaffolds, and affinity membranes.

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Section: Introductionmentioning

confidence: 99%

Dual‐functional electrospun poly(2‐hydroxyethyl methacrylate)

Zhang

Lalani

Cheng

et al. 2011

J Biomedical Materials Res

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“…[1][2][3][4]. Poly (2-hydroxyethyl methacrylate) (pHEMA) is a suitable compatible biomaterial [5,6] and a good candidate for bone implantation [7][8][9][10]. The material can be prepared by bulk polymerization with low water content (and has hardness similar to bone), or by suspension polymerization to form microbeads [11,12].…”

Section: Introductionmentioning

confidence: 99%

Biodegradability of poly (2-hydroxyethyl methacrylate) in the presence of the J774.2 macrophage cell line

et al. 2004

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“…The surface carbohydrates of normal and/or malignant cells participate in the recognition of natural and artificial surfaces including adhesion of leukocytes to the endothelial cells of blood vessels [2][3][4][13][14][15]. The inhibitory effect of the macrophages spreading on the expression cannot be explained but it is known that spreading of the cells participates in the control of the cell function including gene expression [16].…”

Section: Resultsmentioning

confidence: 99%

Distribution of polylactosamines and vinculin in macrophages and foreign body giant cells colonizing the cellophane implant surface

Smetana

Fischer

1995

J Mater Sci: Mater Med

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The membrane poly-N-acetyllactosamines participate in the control of the adhesivity of macrophages and/or monocytes. They have been visualized by FITC labelled tomato lectin in macrophages and multinucleate foreign body giant cells colonizing a cellophane implant in this study. The maximal positivity was found in the periphery of poorly spread macrophages. The multinucleate cells exhibited positivity also in the dorsal surface. The cytoskeletal protein vinculin represents a part of adhesive plaques and participates in anchoring actin fibres to the integrin receptor. The immunohistochemical detection of this protein in the same model indicated poor accumulation of vinculin in the ventral membrane of mononuclear macrophages and multinucleate cells. The increased incidence of this protein was observed in the area of mutual contact between some macrophages.

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Implantation of p(HEMA)-collagen composite into bone

Cited by 35 publications

References 10 publications

Dual‐functional electrospun poly(2‐hydroxyethyl methacrylate)

Dual‐functional electrospun poly(2‐hydroxyethyl methacrylate)

Biodegradability of poly (2-hydroxyethyl methacrylate) in the presence of the J774.2 macrophage cell line

Distribution of polylactosamines and vinculin in macrophages and foreign body giant cells colonizing the cellophane implant surface

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