Implantation of mouse histocompatibility antigens into membranes of cultured tumor cells

Abstract

Membranes of murine lymphoma cells expressing H-2a antigens were isolated, purified and co-reconstituted with isolated Sendai virus envelopes according to a previously published procedure (Volsky, D.J. et al., Proc. Natl. Acad. Sci. USA 1979. 76: 5440.). The resulting hybrid H-2a/Sendai virus envelope vesicles (SH-2a vesicles) were capable of binding to and fusing with mouse lymphoma cells. The fusion resulted in the implantation of H-2a antigens into membranes of target cells, as demonstrated by the presence of serologically active antigens on cultured cells 8 and 16 h after implantation.

Detection of Tumour‐Associated Transplantation Antigens by Syngeneic Cytotoxic T Lymphocytes after Fusion of Tumour Cell Membrane Vesicles to Recipient Cells

“…Fusion techniques have also heen applied to study the interaction of target cells with specific cytotoxie T lymphocytes (CTL) [6,15,18]. Thus, it was shown that H-2 molecules can be implanted and be recognized as target structures by CTL [6,18] or alloantibodies [21]. Similarly, transfer of T-cell receptors required for recognition and lysis of target cells to non-competent recipient cells has recently been demonstrated to generate competent effector CTL [7].…”