Implantation of a Matrigel-loaded agarose scaffold promotes functional regeneration of axons after spinal cord injury in rat

Han, Sungmin; Lee, Jee Youn; Heo, Eun Young; Kwon, Il Keun; Yune, Tae Young; Youn, Inchan

doi:10.1016/j.bbrc.2018.01.157

Cited by 24 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this reason, researchers and clinicians are making great efforts to develop novel therapeutic approaches to stabilize the lesion area and reconnect neural axons in order to regain functionality. Most of these novel approaches are based on drug delivery, , neurotrophic factors, , and cell therapy. , Alternative promising candidates are implantable devices made from a wide range of biomaterials (such as alginate, agarose, and peptides, either alone or in combination with other strategies) aimed to mimic the local inherent tissue environment . In this line, advances in the development of more efficient neural interfaces are still required.…”

Section: Introductionmentioning

confidence: 99%

“…Most of these novel approaches are based on drug delivery, 9,10 neurotrophic factors, 11,12 and cell therapy. 13,14 Alternative promising candidates are implantable devices made from a wide range of biomaterials (such as alginate, 15 agarose, 16 peptides, 17 either alone or in combination with other strategies) aimed to mimic the local inherent tissue environment. 18 In this line, advances in the development of more efficient neural interfaces are still required.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Graphene Oxide Microfibers Promote Regenerative Responses after Chronic Implantation in the Cervical Injured Spinal Cord

Domínguez‐Bajo

González‐Mayorga

López-Dolado

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Spinal cord injury (SCI) is characterized by the disruption of neuronal axons and the creation of an inhibitory environment for spinal tissue regeneration. For decades, researchers and clinicians have been devoting a great effort to develop novel therapeutic approaches which include the fabrication of biocompatible implants that could guide neural tissue repair in the lesion site in an attempt to recover the functionality of the nervous tissue. In this context, although fiberlike structures have been hypothesized to serve as a topographical guidance for axonal regrowth, work on the exploration of this type of materials is still limited for SCI. Aiming to develop such guidance platforms, we recently designed and explored in vitro reduced graphene oxide materials in the shape of microfibers (rGO-MFs). After preliminary studies to assess the feasibility of their implantation at the injured spinal cord in vivo, no evident signs of subacute local toxicity were noticed (10 days of implantation). In this work, we specifically examine for the first time the regenerative potential of these scaffolds, slightly modified in their fabrication for improved reproducibility, when chronically interfaced with a cervical spinal cord injury. After extensive characterization of their physicochemical properties and in vitro experiments with neural progenitor cells, their neural regenerative capacity in vivo is investigated in a rat experimental model of SCI after 4 months of implantation (chronic state). Behavioral tests involving the use of forelimbs are performed. Immunofluorescence studies evidence that rGO-MFs scaffolds foster the presence of neuronal structures along with blood vessels both within the epicenter and in the surroundings of the lesion area. Moreover, the inflammatory response does not worsen by the presence of this material. These findings outline the potential of rGO-MF-based scaffolds to promote regenerative features at the injured spinal cord such as axonal and vascular growth. Further studies including biological functionalization might improve their therapeutic potential by a synergistic effect of topographical and chemical cues, thus boosting neural repair after SCI.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Graphene Oxide Microfibers Promote Regenerative Responses after Chronic Implantation in the Cervical Injured Spinal Cord

Domínguez‐Bajo

González‐Mayorga

López-Dolado

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Matrigel matrix gum is an extract from EHS tumor,its main components are laminin, type IV collagen, basilar proteoglycan, growth factor and so on. It is a hydrogel matrix with a diameter of 20-50 nm, Therefore, cells attached to multiple microporous scaffolds can be fully contacted with materials to form a three-dimensional culture microenvironment (Hill & Sarkar, 2017;Han et al, 2018). In addition to promoting cell proliferation, differentiation and collagen secretion, Matrigel matrix adhesive, as a kind of extracellular matrix complex, also has good mechanical properties and good gelation Matrigel matrix colloid exists in a liquid form at 4°C.…”

Section: Discussionmentioning

confidence: 99%

Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions

et al. 2019

View full text Add to dashboard Cite

Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein,its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three dimensional matrix with biological activity. It can simulate the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of the cells in vitro, and can be used for the study of cell morphology, biochemical function, migration, infection and gene expression. In this study,Matrigel three-dimensional culture model of bone marrow mesenchymal stem cells(BMSCs) was established, and its morphology, proliferation and survival were observed. BMSCs were isolated and cultured with whole bone marrow adherence method. The Second generation BMSCs with good growth condition were selected and mixed with Matrigel to form cell gel complexes. The morphology and proliferation of mesenchymal stem cells were observed by phase contrast microscope and HE staining,Live/Dead staining was used to evaluate the cell activity.Phase contrast microscopy showed that BMSCs were reticulated in Matrigel and proliferated well, After 7 days, the matrix gel gradually became soft and collapsed, a few cell reticular crosslinking growth was seen at 14 days; HE staining showed that the cytoplasm of the cells was larger on the fourth day and the cells were elongated and cross-linked on the seventh day; Live/dead staining showed that most cells showed green fluorescence with the prolongation of culture time, on the first, 4 and 7 days, the activity of bone marrow mesenchymal stem cells in Matrigel gradually increased, and the percentages were 92.57 %, 95.54 % and 97.37 %, respectively. Matrigel three-dimensional culture system can maintain the morphology, function and proliferation ability of bone marrow mesenchymal stem cells.

show abstract

“…In addition, appropriate modification can engineer MSCs secretome directly [142] . Viewing on the cure of spinal cord trauma and regeneration of injured tissue, some biomaterials themselves also have the intrinsic therapeutic capability, including inhibiting glial scar formation, subsiding inflammation, stimulating angiogenesis and promoting neurogenesis [143 , 144] . For instance, HA has immanent favorable functions in CNS tissue repair and regeneration.…”

Section: Mscs Combined With Biomaterials In the Repair Of Scimentioning

confidence: 99%

Biomaterials reinforced MSCs transplantation for spinal cord injury repair

2022

Asian Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Implantation of a Matrigel-loaded agarose scaffold promotes functional regeneration of axons after spinal cord injury in rat

Cited by 24 publications

References 21 publications

Graphene Oxide Microfibers Promote Regenerative Responses after Chronic Implantation in the Cervical Injured Spinal Cord

Graphene Oxide Microfibers Promote Regenerative Responses after Chronic Implantation in the Cervical Injured Spinal Cord

Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions

Biomaterials reinforced MSCs transplantation for spinal cord injury repair

Contact Info

Product

Resources

About