Implantable Patch Sensor for l-Glutamate in Hippocampal Slices

Soma, Natsumi; Watanabe, N.; Shoji, Atsushi; Sugawara, Masao

doi:10.2116/analsci.29.181

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Although the lifetime was shorter than the 40 -50 min for the submerged case, we succeeded in monitoring release of L-glutamate in the CA1 region of mouse hippocampal slices under chemical stimulation with γ-aminobutyric acid (GABA) and potassium chloride (KCl). 136 …”

Section: ·2 Implantable Patch Sensormentioning

confidence: 99%

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

2014

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119 IntroductionTranslocation of ions and small molecules across barriers of cellular membrane, lipid bilayers and solid supports is elicited by biological and artificial pore-forming compounds, which comprise protein, peptide and artificial molecules. Application of nanopores to biosensing holds much promise in developing highly sensitive methods because of their inherent ability of signal transduction and amplification.Nanopore-forming compounds, such as receptor ion-channels, channel-forming peptides and synthetic channels embedded in spherical and planar lipid bilayers ( Fig. 1), have been exploited as sensing elements for the development of biosensors and their uses for biosensing have been reviewed extensively. 1-13Receptor ionchannels, such as glutamate receptor ion channels, possess a 2014 © The Japan Society for Analytical Chemistry Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan Pore-forming compounds are attracting much attention due to the signal transduction ability for the development of highly sensitive biosensing. In this review, we describe an overview of the recent advances made by our group in the design of molecular sensing interfaces of spherical and planar lipid bilayers and natural bilayers. The potential uses of poreforming compounds, such as gramicidin and MCM-41, in lipid bilayers and natural glutamate receptor channels in biomembrane are presented.

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Section: ·2 Implantable Patch Sensormentioning

confidence: 99%

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

2014

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“…Combination of electrophysiological and sensor techniques provides a useful, often essential information on the targeted neuronal events. Recordings of L-glutamate currents at electrochemical sensors have been reported by using different type of electrodes such as an excised patch technique [28,23,22,20,11,37], a whole-cell technique using astrocyte transporter currents [21,12], and an in vivo method using a dialysis electrode [13]. Enzyme-based sensors have also been extensively used for in vivo and in vitro monitoring of extracellular L-glutamate [7,26,35,34,39,38,32,17,33].…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Ikegami

Hozumi

Shoji

et al. 2014

Sensing and Bio-Sensing Research

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Keywords:L-glutamate sensor High-frequency stimulation Field excitatory postsynaptic potential Mouse hippocampal slice Long-term potentiation a b s t r a c t Real-time monitoring of L-glutamate released by high-frequency stimulation in region CA1 of mouse hippocampal slices was performed with a glass capillary-based sensor, in combination with the recoding of excitatory postsynaptic potentials (fEPSPs). A method for extracting L-glutamate currents from the recorded ones was described and applied for determining the level of extracellular L-glutamate released by 100 Hz stimulation. Recording of an L-glutamate current with a current sampling interval of 1 Hz was found to be useful for acquiring a Faradaic current that reflects L-glutamate level released by the highfrequency stimulation of 7 trains, each 20 stimuli at 100 Hz and inter-train interval of 3 s. The L-glutamate level was obtained as 15 ± 6 lM (n = 8) for the persistent enhancement of fEPSPs, i.e., the induction of longterm potentiation (LTP), and 3 ± 1 lM (n = 5) for the case of no LTP induction. Based on these observations, the level of the extracellular L-glutamate was shown to play a crucial role in the induction of LTP.

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Transmembrane Signaling with Lipid‐Bilayer Assemblies as a Platform for Channel‐Based Biosensing

Sugawara

2017

The Chemical Record

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Artificial and natural lipid membranes that elicit transmembrane signaling is are useful as a platform for channel-based biosensing. In this account we summarize our research on the design of transmembrane signaling associated with lipid bilayer membranes containing nanopore-forming compounds. Channel-forming compounds, such as receptor ion-channels, channel-forming peptides and synthetic channels, are embedded in planar and spherical bilayer lipid membranes to develop highly sensitive and selective biosensing methods for a variety of analytes. The membrane-bound receptor approach is useful for introducing receptor sites on both planar and spherical bilayer lipid membranes. Natural receptors in biomembranes are also used for designing of biosensing methods.

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Implantable Patch Sensor for l-Glutamate in Hippocampal Slices

Cited by 3 publications

References 26 publications

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Transmembrane Signaling with Lipid‐Bilayer Assemblies as a Platform for Channel‐Based Biosensing

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