Implantable Microimaging Device for Observing Brain Activities of Rodents

Ohta, Jun; Ohta, Yasumi; Takehara, Hiroaki; Noda, Toshihiko; Sasagawa, Kiyotaka; Haruta, Makito; Kobayashi, Takuma; Akay, Yasemin M.; Akay, Metin

doi:10.1109/jproc.2016.2585585

Cited by 41 publications

(32 citation statements)

References 31 publications

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“…Due to ultra-compactness and cost-effectiveness of the CMOS image sensor chips, implantable devices for the evaluations of biological information 2017) inside living bodies may be promising applications of contact fluorescence microscopy, particularly for brain research, 32,33 glucose monitoring, 34,35 and optical theranostics. 36 In conclusion, we have developed an on-chip cell analysis platform with the integration of contact fluorescence microscopy and microfluidics.…”

Section: Discussionmentioning

confidence: 99%

On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips

et al. 2017

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Although fluorescence microscopy is the gold standard tool for biomedical research and clinical applications, their use beyond well-established laboratory infrastructures remains limited. The present study investigated a novel on-chip cell analysis platform based on contact fluorescence microscopy and microfluidics. Combined use of a contact fluorescence imager based on complementary metal-oxide semiconductor technology and an ultra-thin glass bottom microfluidic chip enabled both to observe living cells with minimal image distortion and to ease controlling and handling of biological samples (e.g. cells and biological molecules) in the imaged area. A proof-of-concept experiment of on-chip detection of cellular response to endothelial growth factor demonstrated promising use for the recently developed on-chip cell analysis platform. Contact fluorescence microscopy has numerous desirable features including compatibility with plastic microfluidic chips and compatibility with the electrical control system, and thus will fulfill the requirements of a fully automated cell analysis system.

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Section: Discussionmentioning

confidence: 99%

On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips

et al. 2017

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“…The problem may be addressed by inserting a glass rod based lens into the brain; however, a large area of lesions can also be induced at the same time. To minimize the invasion, our approach is to combine a customized Si CMOS imaging sensor and micro‐LEDs onto a flexible thin sheet so that it can be implanted into the tissue for high‐resolution deep‐brain fluorescence imaging . The micro‐LEDs adjacent to the imaging sensor serve as light sources to excite green fluorescent protein expressing cells, with an imaging sensor to capture and process fluorescence signals.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

“…f) InGaN blue micro‐LEDs monolithically integrated on Si based neural probes . g) An implantable microimaging probe with micro‐LEDs and a Si CMOS image sensor interconnected on a flexible substrate . h) Fluorescence images obtained from the deep brain of a mouse .…”

Section: Biomedical Applicationsmentioning

confidence: 99%

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Recent Advances in Biointegrated Optoelectronic Devices

Yin

Liu

et al. 2018

Advanced Materials

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With recent progress in the design of materials and mechanics, opportunities have arisen to improve optoelectronic devices, circuits, and systems in curved, flexible, stretchable, and biocompatible formats, thereby enabling integration of customized optoelectronic devices and biological systems. Here, the core material technologies of biointegrated optoelectronic platforms are discussed. An overview of the design and fabrication methods to form semiconductor materials and devices in flexible and stretchable formats is presented, strategies incorporating various heterogeneous substrates, interfaces, and encapsulants are discussed, and their applications in biomimetic, wearable, and implantable systems are highlighted.

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“…(9,14) This makes the imaging system much smaller and more compact and gives a wider field of view while maintaining relatively good resolution. (4,7,9) Previous research has already shown that the fabrication of small yet high-resolution image sensors is possible for various purposes, such as observing the brain activity of rodents, (15) illuminating and detecting light in deep biological tissues for optical therapy and diagnosis, (16) and optogenetics. (17) Moreover, it is possible to insert a small image sensor in an incubator to observe cultured cells for a long period of time, (3,6,8,11,12) and also possible to develop a similar image sensor for long-term Förster resonance energy transfer (FRET) observation in a small animal.…”

Section: Introductionmentioning

confidence: 99%

Lens-free Dual-color Fluorescent CMOS Image Sensor for F?rster Resonance Energy Transfer Imaging

Hee¹,

Sasagawa²,

Kameyama³

et al. 2019

Sensors and Materials

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In this study, a lens-free dual-color bio-imaging system is developed. This system utilizes a small CMOS image sensor chip as the imager. By coupling alternate lines of blue and yellowgreen filters coated on photodiodes, we can simultaneously detect cyan and yellow fluorescent lights using the device. By equipping the device with a hybrid filter made of a bandpass interference filter, a fiber optic plate (FOP), and a pale-yellow absorption filter, we can achieve an excitation light extinction ratio of approximately −57 dB. By using fluorescent beads of two different colors, we can confirm the operation of the image sensor. Individual images from the cyan and yellow channels can be obtained after data processing, and subsequently the yellow-tocyan image ratio can also be determined. The results show that our device can be used to study Förster resonance energy transfer (FRET) and can thus be applied to investigate the progression of diseases such as cancer.

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Implantable Microimaging Device for Observing Brain Activities of Rodents

Cited by 41 publications

References 31 publications

On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips

On-chip cell analysis platform: Implementation of contact fluorescence microscopy in microfluidic chips

Recent Advances in Biointegrated Optoelectronic Devices

Lens-free Dual-color Fluorescent CMOS Image Sensor for F?rster Resonance Energy Transfer Imaging

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