Implantable Flexible Multielectrode Arrays for Multi-Site Sensing of Serotonin Tonic Levels

Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix

“…[3][4][5][6] In contrast, electrochemical techniques based on implantable microprobes with an array of electroenzymatic sensing sites offers a means for multianalyte sensing at high temporal as well as spatial resolution and has developed rapidly into a robust analytical technique for neurotransmitters over the past few years. [7][8][9][10][11] Electroenzymatic sensors typically rely on specific oxidase enzymes to catalyze oxidations of the target analyte to produce hydrogen peroxide (H 2 O 2 ) that is oxidized or reduced at an underlying electrode at moderate potential to give a current signal. Development of microelectrode array (MEA) microprobes for multiple neurotransmitter detection consequently requires a method to selectively deposit multiple different enzymes onto specific microelectrode sites of a MEA microprobe.…”