Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene‐Related Peptide

Mi, Jie; Xu, Jiankun; Yao, Zhaolin; Yao, Hao; Li, Ye; He, Xuan; Dai, Bingyang; Zou, Li; Tong, Wenxue; Zhang, Xiaotian; Hu, Ping; Ruan, Ye Chun; Tang, Ning; Guo, Xia; Zhao, Jie; He, Jufang; Qin, Ling

doi:10.1002/advs.202103005

Cited by 57 publications

(59 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bone fracture is one of the most common traumatic injuries [ 1 , 2 ]. By 2050, the annual number of fractures in China will reach 5.99 million which will cost $25.43 billion [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells

Wang¹,

Guo²,

Wang³

et al. 2022

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

“…Bone fracture is one of the most common traumatic injuries [ 1 , 2 ]. By 2050, the annual number of fractures in China will reach 5.99 million which will cost $25.43 billion [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells

Wang¹,

Guo²,

Wang³

et al. 2022

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

“…Significantly higher activity was observed for cortical neurons in the presence of C-RB02 NPs with respect to cells in control conditions and cells exposed to RB02 NPs (Figure b). Electrical stimulation using electrodes can induce calcium signaling in cortical neurons, dorsal root ganglions, and even nonexcitable brain cells . Here, wireless electrical stimulation and intracellular calcium release is achieved by charged NPs.…”

Section: Resultsmentioning

confidence: 99%

Conductive Conjugated Polymer Nanocapacitors for Localized Electrical Neurostimulation

Martínez-Cartagena¹,

Muzzio

Guntnur

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Noninvasive manipulation of cell signaling is critical in basic neuroscience research and in developing therapies for neurological disorders and tissue engineering and regenerative medicine approaches. In this work, biomimetic synthesized conductive copolymer 3,4-ethylenedioxythiophene (EDOT)-Pyrrole nanoparticles (RB02 NPs) were used for wireless and localized stimulation of neurons. 1 H nuclear magnetic resonance was used to monitor the polymerization. RB02 NPs were characterized by Raman spectroscopy, Fourier transform infrared spectroscopy, and dynamic light scattering. The electrochemical properties were characterized by galvanostatic charge−discharge, voltammetry, and electrochemical impedance spectroscopy. For electrical stimulation of neurons, RB02 NPs were charged by applying 1 V to a NP suspension using platinum electrodes. The effect of NPs on ND7/23 neuron hybrid cell line viability was assessed by live/dead staining using flow cytometry. ND7/23 differentiation was evaluated by cell cytoskeleton staining and quantification of morphological parameters such as the dendrite number and length. Primary cortex neuron stimulation was studied by calcium ion influx detectable through the dynamic fluorescence changes of Fluo-4. RB02 NPs presented no toxicity toward ND7/23 cells. Furthermore, charged NPs enhanced cell differentiation at short times after addition (<6 h). Charged RB02 NPs largely increased the cortex neuronal activity. Altogether, biocompatible copolymer EDOT-Pyrrole nanoparticles present great potential for remote control of neural activities.

show abstract

“…In January 2022, Mi et al implanted electrodes into the dorsal root ganglia (DRG) at the L3 and L4 of the rat spine. They found that electrical stimulation of the DRG could activate the Ca 2+ /CaMKII/CREB signaling pathway and action potential, directly promoting the synthesis and release of CGRP, further inducing osteoporotic fracture healing and the formation of H-type blood vessels ( 20 ). Peripheral nerves can secrete neurotransmitters, neuropeptides, neurotrophic factors, among other signaling molecules, which can affect bone metabolism.…”

Section: Quaternary Regulation Theory Of Bone Metabolismmentioning

confidence: 99%

Exploring the effect of the “quaternary regulation” theory of “peripheral nerve-angiogenesis-osteoclast-osteogenesis” on osteoporosis based on neuropeptides

et al. 2022

View full text Add to dashboard Cite

Osteoporosis is a common bone metabolic disease among the middle-aged and elderly, with its high incidence rate and a major cause of disability and mortality. Early studies found that bone metabolic homeostasis is achieved through osteogenesis-osteoclast coupling. Although current anti-osteoporosis drugs can attenuate bone loss caused by aging, they present specific side effects. With the discovery of CD31hi Emcnhi blood vessels in 2014, the effect of H-type blood vessels on bone metabolism has been valued by researchers, and the ternary regulation theory of bone metabolism of “Angiogenesis-Osteoclast-Osteogenesis” has also been recognized. Nowadays, more studies have confirmed that peripheral nerves substantially impact bone metabolism. However, due to the complex function of peripheral nerves, the crosstalk mechanism of “Peripheral nerve-Angiogenesis-Osteoclast-Osteogenesis” has not yet been fully revealed. Neuropeptide serves as signaling molecules secreted by peripheral nerves that regulate blood vessels, osteoblasts, and osteoclasts’ functions. It is likely to be the breakthrough point of the quaternary regulation theory of “Peripheral nerve-Angiogenesis-Osteoclast-Osteogenesis”. Here, we discuss the effect of peripheral nerves on osteoporosis based on neuropeptides.

show abstract

Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene‐Related Peptide

Cited by 57 publications

References 66 publications

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells

Loss of Bcl-3 delays bone fracture healing through activating NF-κB signaling in mesenchymal stem cells

Conductive Conjugated Polymer Nanocapacitors for Localized Electrical Neurostimulation

Exploring the effect of the “quaternary regulation” theory of “peripheral nerve-angiogenesis-osteoclast-osteogenesis” on osteoporosis based on neuropeptides

Contact Info

Product

Resources

About