Implant‐associated osteomyelitis: Development, characterisation, and application of a porcine model

Jensen, Louise Kruse

doi:10.1111/apm.13125

Cited by 8 publications

(4 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paraffin-embedded porcine tissue blocks, originally made for histology, were collected for IHC analyses. Briefly, the porcine model for implant-associated osteomyelitis was made by drilling an implant cavity (4 9 20 mm) in the proximal tibia followed by injection of 10 4 CFU of S. aureus and insertion of a small steel implant (2 9 15 mm) [18]. The collected bone tissue originated from animals euthanized at 5, 10, 15, and 19 days after inoculation, respectively [18].…”

Section: Porcine Tissue Cohortmentioning

confidence: 99%

“…Briefly, the porcine model for implant-associated osteomyelitis was made by drilling an implant cavity (4 9 20 mm) in the proximal tibia followed by injection of 10 4 CFU of S. aureus and insertion of a small steel implant (2 9 15 mm) [18]. The collected bone tissue originated from animals euthanized at 5, 10, 15, and 19 days after inoculation, respectively [18]. The bone tissue of all included animals was confirmed to be infected by the S. aureus strain used for inoculation [18].…”

Section: Porcine Tissue Cohortmentioning

confidence: 99%

“…The collected bone tissue originated from animals euthanized at 5, 10, 15, and 19 days after inoculation, respectively [18]. The bone tissue of all included animals was confirmed to be infected by the S. aureus strain used for inoculation [18]. The experiment was approved by the Danish Animal Experiments Inspectorate (license no.…”

Section: Porcine Tissue Cohortmentioning

confidence: 99%

“…Additionally, a cohort of S. aureus ‐infected bone tissue from a well‐characterized porcine model of osteomyelitis was also examined for OLCN‐located bacteria [18]. The porcine bone tissue had well‐defined infection times of 5, 10, 15, and 19 days, respectively, and was included to elucidate whether a certain infection time is needed to initiate OLCN invasion.…”

Section: Figmentioning

confidence: 99%

See 3 more Smart Citations

Bacterial invasion of the submicron osteocyte lacuna–canaliculi network (OLCN): a part of osteomyelitis disease biology

Jensen

Birch

Elvang

et al. 2023

APMIS

Self Cite

View full text Add to dashboard Cite

Two chronic osteomyelitis patients, a diabetic foot osteomyelitis patient and a fracture‐related infection patient, all with staphylococci‐positive microbiology, were examined to confirm the clinical relevance of bacterial invasion of the submicron osteocyte lacuna–canaliculi network (OLCN) in bone tissue. Based on immunohistochemistry and light microscopy both Staphylococcus aureus and Staphylococcus epidermidis were identified within the OLCN of all four patients. The findings consolidate that bacterial OLCN invasion is a clinically relevant part of osteomyelitis disease biology, which from experimental porcine infections, seems to be time depending. The microscopy pictures of the four patients significantly add to visualize the phenomenon of bacterial OLCN invasion.

show abstract

Section: Porcine Tissue Cohortmentioning

confidence: 99%

Section: Porcine Tissue Cohortmentioning

confidence: 99%

Section: Porcine Tissue Cohortmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial invasion of the submicron osteocyte lacuna–canaliculi network (OLCN): a part of osteomyelitis disease biology

Jensen

Birch

Elvang

et al. 2023

APMIS

Self Cite

View full text Add to dashboard Cite

show abstract

Penicillin concentrations in bone and subcutaneous tissue: A porcine microdialysis study comparing oral and intravenous treatment

Rasmussen,

Hanberg,

Lilleøre

et al. 2024

Journal Orthopaedic Research

View full text Add to dashboard Cite

Penicillin is available in both an oral (penicillin V) and intravenous formulation (penicillin G), theoretically allowing for a safe transition between the two. However, the use of oral penicillin remains a topic of debate due to low and variable bioavailability. This study aimed to assess the time for which the free penicillin concentration exceeded targeted minimum inhibitory concentrations for Staphylococcus aureus and Streptococcus species (0.125, 0.25, and 0.5 mg/L) in cancellous bone and subcutaneous tissue after intravenous penicillin and oral penicillin administration. 12 female pigs (68–75 kg) were assigned, according to local standard clinical regimes, to either intravenous penicillin (1.2 g) or oral penicillin (0.8 g) treatment every 6 h over an 18 h period. Microdialysis catheters were placed for sampling in tibial cancellous bone and adjacent subcutaneous tissue. Data was dynamic/continually collected in the first dosing interval (0–6 h), simulating a prophylactic situation, and the third dosing interval (12–18 h), simulating a therapeutic setting. Plasma samples were collected for reference. For all investigated targets, intravenous treatment resulted in a longer mean time above relevant minimum inhibitory concentrations in cancellous bone during the first dosing interval, and in both cancellous bone and subcutaneous tissue during the third dosing interval compared to oral treatment. With clinically relevant dosing, intravenous penicillin provides superior exposure compared to oral penicillin in both a prophylactic and therapeutic setting.

show abstract

Model of Osteomyelitis in Rats

et al. 2022

View full text Add to dashboard Cite

Implant‐associated osteomyelitis: Development, characterisation, and application of a porcine model

Cited by 8 publications

References 116 publications

Bacterial invasion of the submicron osteocyte lacuna–canaliculi network (OLCN): a part of osteomyelitis disease biology

Bacterial invasion of the submicron osteocyte lacuna–canaliculi network (OLCN): a part of osteomyelitis disease biology

Penicillin concentrations in bone and subcutaneous tissue: A porcine microdialysis study comparing oral and intravenous treatment

Model of Osteomyelitis in Rats

Contact Info

Product

Resources

About