Impedimetric or Ion‐Sensitive Field‐Effect Transistor (ISFET) Aptasensors Based on the Self‐Assembly of Au Nanoparticle‐Functionalized Supramolecular Aptamer Nanostructures

Sharon, Etery; Freeman, Ronit; Tel‐Vered, Ran; Willner, Itamar

doi:10.1002/elan.200804565

Cited by 49 publications

(34 citation statements)

References 39 publications

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“…Sharon et al [26] used aptamer fragments to develop a biosensor for cocaine in a sandwich format. One aptamer fragment was assembled on the electrode surface or ionsensitive field-effect transistor (ISFET) gate, while the second thiolated aptamer fragment was bound to gold nanoparticles (AuNPs).…”

Section: Labelled Aptamer-based Sensorsmentioning

confidence: 99%

Electrochemical aptamer-based sensors

2010

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The valuable properties of aptamers, such as specificity, sensitivity, stability, cost-effectiveness and design flexibility, have favoured their use as biorecognition elements in biosensor development. These synthetic affinity probes can be developed for almost any target molecule, covering a wide range of applications in fields such as clinical diagnosis and therapy, environmental monitoring and food control. The combination of aptamers with highperformance electrochemical transducers, with their inherent high sensitivities, fast response times and simple equipment, has already provided several electrochemical aptamer-based sensors. Moreover, the small size and versatility of aptamers allow efficient immobilisations in high-density monolayers, an important feature towards miniaturisation and integration of compact electrochemical devices. This review describes the state-of-the-art of electrochemical aptamer-based sensors, entering into the details of the different strategies and types of electrochemical transduction and also considering their advantages when applied to the analysis of complex matrices.

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Section: Labelled Aptamer-based Sensorsmentioning

confidence: 99%

Electrochemical aptamer-based sensors

2010

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“…The electrochemical Fig. 13 a Schematic presentation of the analysis of cocaine [118]. b Schematic diagram for Lysozyme or Adenosine Detection without DNA-AuNPs Amplification (a) and with DNA-AuNPs Amplification (b).…”

Section: Aunps As Probe Vectors In Electrochemical Aptasensorsmentioning

confidence: 99%

“…13a) [118]. The methods utilized CBA fragments that selfassemble to a supramolecular aptamer fragments/cocaine complex on the electrode surface in the presence of cocaine.…”

Section: Aunps As Probe Vectors In Electrochemical Aptasensorsmentioning

confidence: 99%

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Wang

2010

Bioanal Rev

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Gold nanoparticles (AuNPs) exhibit many predominant capabilities such as high biocompatibility, chemical stability, strong localized surface plasmon resonance absorption, and high extinction coefficient in the visible region. These properties have enabled the extensive use of AuNPs in optical and electrochemical biosensors. As a kind of functional nucleic acids, aptamers can be considered as a valid alternative to antibodies or other bio-receptors and have been widely employed to develop novel biosensors. We are summarizing here the state of the art of AuNP-based biosensors that use functional aptamers as molecular recognition elements. In many cases, AuNPs themselves can be used as a probe for detection, such as various colorimetric aptasensors and fluorescent aptasensors. They also can be used as probe vectors to enlarge detection signals and to increase the number of conceivable substrates in electrochemical aptasensors.

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“…The second thiolated aptamer fragment was bound to gold nanoparticles. A sandwich coupling of substrate with two aptamers caused a change in the interfacial electron transfer resistance and changed the potential of the ISFET device [191].…”

Section: Potentiometric Biosensorsmentioning

confidence: 99%