Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli

Cheng, Ming Shien; Ho, Jia Shin; Lau, Suk Hiang; Chow, Vincent T. K.; Toh, Chee-Seng

doi:10.1016/j.bios.2013.03.050

Cited by 13 publications

(4 citation statements)

References 28 publications

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“…Isothermal amplification and RT-PCR, generate very rapid results, saving hours of sample analysis time and can be developed inexpensively into sensitive, specific tools that are easy to operate. Biosensors may be seen as a future gold standard for virus detection as they potentially can be developed inexpensively into simple, sensitive and specific analytical tools with the ability of very rapid analysis of multiple samples compared to the conventional methods (Altintas & Tothill 2013, Cheng et al, 2013. However, more often there are obstacles with recognition molecules such as steric hindrance, denaturation and non-specific binding to nontarget molecules.…”

Section: Discussionmentioning

confidence: 99%

“…The limitations of these as POCT methods are currently their high operation costs and lower specificity/sensitivity due to the possibilities of non-specific binding or hybridization that may occur during amplification. To date microarray applications are limited to certain laboratories (Cheng et al, 2013) but there is increasing awareness and interest in these arrays or panels for multiplex analysis.…”

Section: Discussionmentioning

confidence: 99%

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Current state-of-the-art diagnostics for Norovirus detection: Model approaches for point-of-care analysis

Zaczek-Moczydlowska

Beizaei²,

Dillon

et al. 2021

Trends in Food Science & Technology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Current state-of-the-art diagnostics for Norovirus detection: Model approaches for point-of-care analysis

Zaczek-Moczydlowska

Beizaei²,

Dillon

et al. 2021

Trends in Food Science & Technology

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“…It is a three-electrode system wherein the working and counter electrodes are made of gold, while the reference electrode and electric contacts are made of silver. The working electrode area of the Au-SPE was pre-treated as reported in related literature [15,19]. Gold disk electrodes were polished with 0.3 and 0.5 µm alumina slurry, rinsed with water, and dried under flowing nitrogen gas.…”

Section: Preparation Of Bacteriophagementioning

confidence: 99%

Electrochemical impedance spectroscopy (EIS)-based measurement of the binding and lysis activity of immobilized bacteriophage UP87 on Aeromonas hydrophila

Dujunco¹,

Jr.²,

Cruz-Papa³

et al. 2021

actamanil

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In this study, a measurement system based on Electrochemical Impedance Spectroscopy (EIS) was assembled to determine whether bacteriophage UP87 immobilized on a gold electrode surface would exhibit binding and lytic activity towards Aeromonas hydrophila. Bacteriophage UP87, a lytic phage for A. hydrophila, was immobilized on gold screen-printed electrodes (Au-SPEs), which contained a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (MUA) and dithiothreitol (DTT), via carbodiimide cross-linker chemistry. The phage solution incubation time on the Au SPE was optimized to ensure maximum phage coverage of the electrode. The optimum time for phage UP87 immobilization was 60 minutes. To confirm the successful immobilization of the phage on the electrode surface, the Au-SPEs were characterized using Fourier Transform Infrared Spectroscopy (FTIR). The study of the binding and lysis of A. hydrophila was done through bacterial solution incubation on the treated Au-SPEs for different durations. Binding of the bacterial cells was shown by the general increase in the charge transfer resistance (R ct ) up to a maximum value obtained after 60 minutes of incubation. Lysis of the captured bacterial cells was indicated through the decrease in R ct value after 80 minutes of incubation. In addition, the phage-modified Au-SPE exhibited higher R ct values at 60 minutes of incubation period for A. hydrophila compared with the non-target bacteria Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, thus showing satisfactory selectivity. This high selectivity was attributed to the pre-treatment of the Au-SPE which blocked nonspecific interactions and highly favored the binding of A. hydrophila. The results of this study indicate that surface immobilization of phages can be used to concentrate them for binding and lysing bacteria in solution. The concentration-dependence of the bacteria's binding to the phage-modified surface hints at the possibility of using the phage UP87 as a biorecognition element in a selective biosensor for A. hydrophila using the EIS technique.

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“…Traditional plaque assays have been extensively used in the detection and quantification of coliphages in water (Grabow and Coubrough, 1986). Despite their usage widely, their application is limited by the time-consuming procedures, tediousness in real time operation and it is not feasible for the detection of non-lytic phages (Cheng et al, 2013). While for the detection of fecal indicators, detection methods include the multiple tube fermentation (method of most probable number) or membrane filtration (Rompré et al, 2002).…”

Section: A11 Backgroundmentioning

confidence: 99%

Development of monitoring tools based on electrical impedance spectroscopy for water treatment and integrated process for wastewater recovery

Ho¹

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Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli

Cited by 13 publications

References 28 publications

Current state-of-the-art diagnostics for Norovirus detection: Model approaches for point-of-care analysis

Current state-of-the-art diagnostics for Norovirus detection: Model approaches for point-of-care analysis

Electrochemical impedance spectroscopy (EIS)-based measurement of the binding and lysis activity of immobilized bacteriophage UP87 on Aeromonas hydrophila

Development of monitoring tools based on electrical impedance spectroscopy for water treatment and integrated process for wastewater recovery

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