Impedimetric biosensor of Norovirus with low variance using simple bioconjugation on conductive polymer-Au nanocomposite

Nasrin, Fahmida; Khoris, Indra Memdi; Chowdhury, Ankan Dutta; Boonyakida, Jirayu; Park, Enoch Y.

doi:10.1016/j.snb.2022.132390

Cited by 13 publications

(10 citation statements)

References 36 publications

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“…Based on our previous optimizations, [ 37 ] 0.6 m of HAuCl 4 is suited to the best oxidation condition with 0.1 m of aniline monomer at 20°C temperature, where the best quality of the nanocomposites has been synthesized for the sensing application. The TEM image shows the AuNP‐PAni nanocomposite in nanotube formation (Figure S1), where the intense black spot represents the homogeneously distributed AuNP.…”

Section: Resultsmentioning

confidence: 99%

“…To achieve better conjugation and the orientation of the antibody on the electrode surface, a more conventional but versatile conjugation method of the AuNP/streptavidin with a biotinylated‐antibody has been introduced. [ 37 ] In this work, we synthesized the Au‐PAni using interfacial polymerization method, where the antibody was bound on the Au‐PAni coated screen‐printed carbon electrode (SPCE) via streptavidin–biotin bioconjugation. The antibody‐specific SPCEs were utilized for the impedimetric detection of the flavivirus family, CHIKV and ZIKV.…”

Section: Introductionmentioning

confidence: 99%

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Development of disposable electrode for the detection of mosquito‐borne viruses

Nasrin

Khoris

Chowdhury

et al. 2023

Biotechnology Journal

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Development of disposable, rapid, and convenient biosensor with high sensitivity and reliability is the most desired method of viral disease prevention. To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen‐printed carbon electrode (SPCE) for the detection of two mosquito‐borne viruses. The biosensor fabrication has step‐wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)‐polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP‐PAni/PAni‐SPCE via the streptavidin‐biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody–antigen interaction, this disposable electrode has designed to detect mosquito‐borne infectious viruses, Chikungunya virus (CHIKV), and Zika virus (ZIKV) separately in a wide linear range of 100 fg mL−1 to 1 ng mL−1 with a low detection limit of 1.33 and 12.31 fg mL−1, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of disposable electrode for the detection of mosquito‐borne viruses

Nasrin

Khoris

Chowdhury

et al. 2023

Biotechnology Journal

Self Cite

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“…However, concentration dependences are usually determined relative to ΔRct. The initial modified working electrode of the biosensor must have sufficiently good electrical conductivity to ensure the process of charge transfer between the electrode and the electrolyte; therefore, conductive materials are used in the formation of the electrode or electrode-modifying material [43][44][45]. Thus, the electrochemical system for faradaic EIS is typically represented as a Randle equivalent circuit, which contains the electrolyte resistance R s between the biosensor (WE) and the reference electrode (RE), the electrical double-layer capacitance C dl (or constant phase element (CPE)), charge transfer resistance R ct , and the Warburg element (Z W ) (Figure 2b).…”

Section: Basic Principles Of Impedimetric Measurementsmentioning

confidence: 99%

“…However, concentration dependences are usually determined relative to ∆R ct . The initial modified working electrode of the biosensor must have sufficiently good electrical conductivity to ensure the process of charge transfer between the electrode and the electrolyte; therefore, conductive materials are used in the formation of the electrode or electrode-modifying material [43][44][45].…”

Section: Basic Principles Of Impedimetric Measurementsmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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“…[21][22][23] Within such materials, nanostructured conductive polymers have emerged as interesting contributors to this field. 24,25…”

Section: Introductionmentioning

confidence: 99%