Impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and CramoLL lectin for bacterial lipopolysaccharide recognition

Abstract

We report the development of a new selective and specific electrochemical biosensor for bacterial lipolysaccharide (LPS). An electrode interface was constructed using a l-cysteine-gold nanoparticle (AuNpCys) composite to be immobilized by electrostatic interaction in the network of a poly(vinyl chloride-vinyl acetate maleic acid) (PVM) layer on a gold bare electrode. The impedimetric biosensor is fabricated by self-assembled CramoLL lectin on the PVM-AuNpCys-modified gold electrode through electrostatic interaction. CramoLL is used as the recognition interface. AFM images showed that LPS was specifically recognized on the PVM-AuNpCys-CramoLL system surface. The measurements of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed that the electrochemical response of a redox probe system (K(4)[Fe(CN)(6)](4-)/K(3)[Fe(CN)(6)](3-)) were blocked, due to the procedures of modified electrode with PVM-AuNpCys-CramoLL. In the majority of the experiments the lectin retained its activity as observed through its interaction with LPS from Escherichia coli, Serratia marcescens, Salmonella enterica and Klebsiella pneumoniae. The results are expressed in terms of the charge transfer resistance and current peak anodic using the EIS and CV techniques for the development of a biosensor for contamination by endotoxins. A new type of sensor for selective discrimination of LPS types with a high sensitivity has been obtained.

“Clicked” galactosyl anthraquinone on graphene electrodes for the label-free impedance detection of live cancer cells

“…Electrochemical impedance spectroscopy (EIS) is a technique sensitive to the charge transfer resistance of the detection interface. With EIS the detection of proteins, cells and pathogens has been made possible in a label-free manner [12][13][14][15][16][17][18][19][20]. This study demonstrates the use of EIS for the label-free detection of a live cancer cell based on specific sugar-receptor recognitions.…”

“…These receptors can be viewed as good biomarkers for the direct capture of live cells since they distribute at the transmembrane location and can thus interact with extracellular stimuli. EIS sensors for sugar receptor detection have been developed [12][13][14][15][16][17][18][19][20].…”

Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor

“…Structural analysis of the sensor was performed by an atomic force microscope (Agilent AFM, Picoplus Molecular Imaging, USA) [7]. Cantilevers with a silicon AFM probe (Multi 75AL, NCHR, resonant frequency = 75 kHz, force constant = 3 N m À1 ) were used for the noncontact mode AFM in air at room temperature (approximately 25°C).…”

“…It is important to note that the applicability of electrochemical impedance spectroscopy (EIS) in the development of biodevices has been increasing [6][7][8][9]. EIS has become one of the most used techniques for the development of biosensors since it is a useful tool to evaluate the interactions between biomolecules.…”

CdTe quantum dots as a novel biosensor for Serratia marcescens and Lipopolysaccharide

“…Serratia marcescens, a Gram-negative bacterium of the family Enterobacteriaceae is commonly found in soil, water and food, where it is considered to be a harmless indigenous bacterium [20]. Serratia marcescens exhibits a linear polysaccharide composed of D-glucose-L-rhamnose disaccharide, while the Lipid A moiety of LPS is one of the essential factors of pneumonia caused by Serratia marcescens infection [20][21]. LPS binding to Con A has been proven and can be used as a biorecognition element for LPS biosensor [19,21].…”