Impeded Establishment of the Infective Stage of Trichinella in the Intestinal Mucosa of Mice by Passive Transfer of an IgA Monoclonal Antibody

Abstract

ABSTRACT. Our previous study showed that the IgA monoclonal antibody (mAb) HUSM-Tb1 forms immunoprecipitates on the cuticular surface of infective larvae of Trichinella britovi, and that intraperitoneal injection of this mAb to mice 5 hr before challenge infection confers a high level of protection against intestinal T. britovi. The same treatment produced a similar effect in BALB/c mice inoculated orally with Trichinella pseudospiralis larvae, indicating that the effects may be seen upon most members of the genus Trichinella. Worms recovered from the intestinal mucosa at 1 hr after challenge infection with T. pseudospiralis was few in mice passively immunized with the mAb, whereas a substantial number of worms were recovered from the mucosa of control groups. These results suggest that the IgA mAb impedes establishment of infective Trichinella worms in the intestinal mucosa. Trichinella worms inoculated orally into BALB/c mice vaccinated with ultraviolet-irradiated muscle larvae 3 weeks earlier were expelled between days 4 and 7 after challenge infection. Although the mAb HUSM-Tb1 originated from the mesenteric lymph node cells of mice vaccinated repeatedly with such irradiated larvae, IgA-mediated expulsion does not seem to play an important role in this vaccination model.

Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA

“…The sIgA exerts a key function in intestinal defense and blocking of parasite invasion of intestinal epithelium. The sIgA against adult surface antigens mediated intestinal adult worm expulsion, passive transfer of McAb IgA against Trichinella to naive mice produced 95% protection against larval challenge infection [ 54 , 55 ]. Besides, the protection might be due to the formation of anti- T. spiralis antibody immune complex in the larval head, which may physically block larval direct contact with IEC, therefore protecting intestinal mucosa from larval invasion [ 18 , 56 , 57 ].…”

The role of the secretory immune response in the infection by Entamoeba histolytica

“…Protection against bacteria such as Neisseria meningitidis (14) , Streptococcus pneumoniae (15), enterotoxigenic Escherichia coli (16) and Bordetella pertussis (17) has been obtained with sIgA antibodies, alone or in combination with immune cells, and protection against the oral deposition of S mutants in mice has been achieved by using recombinant anti‐adherent IgA antibodies produced in plants, suggesting a potential application in limiting dental caries (18). Moreover, immunity in mice against the nematodes Trichiuris muris or Trichinella britovi by the passive transfer of specific sIgA monoclonal antibodies into the intraperitoneal cavity previous to the challenge has also been reported (19,20). Similar role in protection against Giardia muris has been derived from studies in mice genetically deficient for IgA production where the intestinal infection cannot be resolved, in comparison with nondeficient mice which easily resolve the infection during the early stages (21).…”

Protective immunity in mice vaccinated with a novel elastase-1 significantly decreases Trichinella spiralis fecundity and infection

“…Since TsE is a secretory protein that is highly expressed at the T. spiralis intestinal invasive stage (IIL), TsE might be exposed early to the host's intestinal mucosa and elicit the local immune response. Our results indicated that vaccination with rTsE induced significantly high levels of TsE-specific sIgA, which might facilitate adult worm expulsion from the intestine [64]. The immune protection (64.06% ML reduction) with this novel TsE vaccination was superior to those of the abovementioned other T. spiralis proteins as candidate vaccine target molecules.…”

“…sIgA acts an important function in mucosal defense and might protect the intestinal epithelium from parasite intrusion [42,63]. Anti-T. spiralis sIgA mediated intestinal adult expulsion from the gut, and passive transfer of the anti-Trichinella antibody IgA produced evident protection against larval challenge in mice [64]. Our results also indicated that subcutaneous immunization with rTsE induced the mixed Th1/Th2 response, as demonstrated by an obvious elevation of Th1 cytokines (IFN-γ, IL-2) and Th2 cytokines (IL-4, IL-10) after spleen, MLN and PP cells from immunized mice were stimulated with rTsE.…”