Impedance spectroscopy of interfaces, membranes and ultrastructures

Coster, H.G.L.; Chilcott, Terry C.; Coster, Adelle C.F.

doi:10.1016/0302-4598(96)05064-7

Cited by 196 publications

(137 citation statements)

References 72 publications

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“…The detailed mathematical description of DS has been discussed in the literature and in textbooks [Arnold, 2001, Coster et al, 1996, Davey et al, 1993a, Davey et al, 1993b, Marszalek et al, 1990, Pethig and Kell, 1987, Barsoukov and Macdonald, 2005]. An overview is given below.…”

Section: Mathematical Description Of Dielectric Spectroscopymentioning

confidence: 99%

Process control in cell culture technology using dielectric spectroscopy

Justice¹,

Brix

Freimark³

et al. 2011

Biotechnology Advances

106

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Section: Mathematical Description Of Dielectric Spectroscopymentioning

confidence: 99%

Process control in cell culture technology using dielectric spectroscopy

Justice¹,

Brix

Freimark³

et al. 2011

Biotechnology Advances

106

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“…These mobile charges, in the membrane or in the unstirred layers around the membrane, may also distort the results from injection of AC current, and explain why this technique gives higher values than the derivative of the I-V curves (Beilby & Bisson, 1999). Frequency dependence of this response could throw some light on the nature of these charges (Coster et al, 1996). In addition, curvefitting techniques used to deconstruct data into multiple exponentials give apparently good fits even when data have been generated by other models.…”

Section: Electrophysiologymentioning

confidence: 99%

Electrophysiology of Turgor Regulation in Marine Siphonous Green Algae

2006

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Abstract. We review electrophysiological measures of turgor regulation in some siphonous green algae, primarily the giant-celled marine algae, Valonia, and Ventricaria, with particular comparison to the well studied charophyte algae Chara and Lamprothamnium. The siphonous green algae have a less negative plasma membrane potential, and are unlikely to have a proton-based chemiosmotic transport system, dominated by active electrogenic K + uptake. We also make note of the unusual cellular structure of the siphonous green algae. Hypertonic stress, due to increased external osmotic pressure, is accompanied by positive-going potential difference (PD), increase in conductance, and slow turgor-regulation. The relationship between these is not yet resolved, but may involve changes in K + conductance (G K ) or active K + transport at both membranes. Hypotonic turgor regulation, in response to decrease external osmotic pressure, is 3 times faster than hypertonic turgor regulation. It is accompanied by a negative-going PD, although conductance also increases. The conductance increase and the magnitude of the PD change are strongly correlated with the magnitude of hypotonic stress.

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“…Dielectric spectroscopy is the direct measurement of permittivity and conductivity of heterogeneous systems as a function of frequency. 17 Dielectric properties of various biological cells, such as mouse lymphocytes and erythrocytes, 5 human erythrocytes, 6,18,19 normal and malignant white blood cells, 20 and leukemia cells, 21 have been studied by this technique. However, dielectric spectroscopy is limited to the measurements of cell suspensions.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Wu¹,

Yung²,

Lim³

2012

Biomicrofluidics

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In this paper, a new dielectrophoresis (DEP) method based on capture voltage spectrum is proposed for measuring dielectric properties of biological cells. The capture voltage spectrum can be obtained from the balance of dielectrophoretic force and Stokes drag force acting on the cell in a microfluidic device with fluid flow and strip electrodes. The method was demonstrated with the measurement of dielectric properties of human colon cancer cells . From the capture voltage spectrum, the real part of Clausius-Mossotti factor of HT-29 cells for different frequencies of applied electric field was obtained. The dielectric properties of cell interior and plasma membrane were then estimated by using single-shell dielectric model. The cell interior permittivity and conductivity were found to be insensitive to changes in the conductivity of the medium in which the cells are suspended, but the measured permittivity and conductivity of cell membrane were found to increase with the increase of medium conductivity. In addition, the measurement of capture voltage spectrum was found to be useful in providing the optimum operating conditions for separating HT-29 cells from other cells (such as red blood cells) using dielectrophoresis. V C 2012 American Institute of Physics. [http://dx

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Impedance spectroscopy of interfaces, membranes and ultrastructures

Cited by 196 publications

References 72 publications

Process control in cell culture technology using dielectric spectroscopy

Process control in cell culture technology using dielectric spectroscopy

Electrophysiology of Turgor Regulation in Marine Siphonous Green Algae

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

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