Impedance Detection and Modeling of Chemotherapeutic Agents by a Cancer Cell-Based Biosensor

Liu, Qingjun; Lu, Yanli; Wang, Hua; Zhou, Jie; Zhang, Yongsheng; Chen, Qun-wei; Luo, Senbiao; Li, Rong; Wang, Ping

doi:10.1080/00032719.2013.867498

Cited by 2 publications

(4 citation statements)

References 39 publications

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“…The cell index was recorded for another 24 h. The toxicity reflected by the cell index was the gross cytotoxicity of different drugs of different concentrations, which had effects on the cell biological status including cell viability, cell number, morphology, and adhesion. (26)(27)(28)…”

Section: Cell Index Detectionmentioning

confidence: 99%

“…As a powerful method for the characterization of the structural features of the sensing interface, impedance spectra can be used to explain the mechanism of biochemical processes occurring at the electrode-solution interfaces. (26,31) Examination of the impedance changes enables the analysis of interfacial changes originating from biorecognition events or cell adhesion at electrode surfaces and provides real-time and kinetic information of cell behaviors. Thus, the cell-based impedance biosensors could be used to monitor the conditions of cancer cell attachment, cell proliferation, and cell detachment by exposing cells to different medicinal compounds.…”

Section: Impedance Circuit Modelingmentioning

confidence: 99%

“…Compared with traditional viability assays (end-point viability readout), such as the WST-1, the cell-based biosensor, which is simple, sensitive, and low in cost, could be applied for studying the real-time and kinetic information of cell behavior, such as cell proliferation, morphology, and motility. (26,42) The ability of rapidly quantifying the toxicity of potential anti-cancer medicines to targeted cancer cells would provide an efficient method for the rapid screening of drugs in preclinical research.…”

Section: Impedance Model Analysismentioning

confidence: 99%

“…To achieve sufficient detection for interpreting the early effects of drugs on cells for cancer therapy or anti-cancer drug screening, various strategies have been explored. (22)(23)(24)(25)(26) Among them, biosensors based on the electric cellsubstrate impedance sensing (ECIS) technique are promising for monitoring cell proliferation, morphology, motility, and apoptosis induced by different medicines. They can rapidly quantify the toxicity and selectivity of potential medicines to targeted cells, and could be used as a potential alternative to end-point assay in preclinical research, the development of new drugs, and toxicological testing.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Cytotoxic Effects of Chemotherapeutic Agents for Gastrointestinal Cancer with Cell-based Impedance Biosensor

Luo¹,

Zhou²,

Dai³

et al. 2018

Sensors and Materials

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As one of the major causes of cancer-related death, gastrointestinal cancer seriously impacts the quality of human life. Moreover, in the majority of patients, the disease is in an advanced state at the time of diagnosis. The only available treatment for such patients is systemic chemotherapy, which may cause various side effects. Thus, the need for fast evaluation functions of different medicines and developing new drugs have become urgent in recent years. In this study, four different anti-cancer reagents, namely, compound Kushen injection (CKI), cinobufacini injection, transforming growth factor-β1 (TGF-β1), and 5-fluorouracil (5-FU), were each tested with a cell-based biosensor to investigate their therapeutic effects on gastrointestinal cancer cells. From the impedance results, we could infer that chemotherapeutic agents could induce cell apoptosis, disrupt cell cycle, and inhibit cell adhesion capacity. By combining impedance spectra with an appropriate impedance circuit, the function of 5-FU on cell death and adhesion could be preliminarily investigated. Because the impedance sensing is in real time, has high throughput, and is easily manipulated, the developed cell-based impedance biosensor could be used as a suitable and promising alternative to end-point assay in the preclinical research of medicines, including the development of new therapeutic regimens.

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Section: Cell Index Detectionmentioning

confidence: 99%

Section: Impedance Circuit Modelingmentioning

confidence: 99%

Section: Impedance Model Analysismentioning

confidence: 99%