Impedance based sensing of the specific binding reaction between Staphylococcus enterotoxin B and its antibody on an ultra-thin platinum film

DeSilva, Mutha; Zhang, Yu; Hesketh, Peter J.; Maclay, G. Jordan; Gendel, Steven M.; Stetter, Joseph R.

doi:10.1016/0956-5663(95)96958-2

Cited by 90 publications

(36 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, cross-reactivities were explicitly tested and were below 10% except at concentrations below 10 ng/mL, where they were worse. DeSilva et al published an early report using a film of platinum islands coated with an antibody probe as the sensor surface [210] with impedance readout at 100 Hz. Although the reported 0.4 ng/mL limit of detection is impressive, only three sensor surfaces were measured thus little characterization of the nonspecific response could be performed.…”

Section: Special Electrode Surfacesmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

View full text Add to dashboard Cite

Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

show abstract

Section: Special Electrode Surfacesmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

View full text Add to dashboard Cite

show abstract

“…193,200 Impedance measurements at various frequencies allow one to detect antibody-antigen binding. 201,202 In Brazil, the advantages of the use of conductometric detection coupled to flow injection analyis was described by Jardim and co-workers 203 to measure CO 2 in the atmosphere. Fatibello and Borges 204 have also shown a flow injection conductometric system appropriate to evaluate acidity in industrial hydrated ethyl alcohol.…”

Section: Conductometric Sensorsmentioning

confidence: 99%

Electrochemical sensors: a powerful tool in analytical chemistry

Stradiotto¹,

Yamanaka²,

Zanoni³

2003

J. Braz. Chem. Soc.

326

154

View full text Add to dashboard Cite

Sensores potenciométricos, amperométricos e condutométricos são sensores eletroquímicos com imensa aplicação nas áreas de meio ambiente, indústria e análises clínicas. O presente trabalho apresenta uma revisão generalizada dos aspectos fundamentais, os desenvolvimento e a contribuição destes sensores na área de química analítica, e reporta alguns aspectos relevantes dos avanços na área de sensores eletroquímicos no Brasil.Potentiometric, amperometric and conductometric electrochemical sensors have found a number of interesting applications in the areas of environmental, industrial, and clinical analyses. This review presents a general overview of the three main types of electrochemical sensors, describing fundamental aspects, developments and their contribution to the area of analytical chemistry, relating relevant aspects of the development of electrochemical sensors in Brazil.

show abstract

“…Recently, it has received attention from researchers in other fields due to its ability of rapid measurement for bacterial concentrations [4][5][6][7]. In some studies, the dielectrophoretic impedance measurement method was combined with antigen-antibody reaction [6,8,9]. In other studies, the electrical impedance measurements were used to detect the ionic metabolites produced during the bacterial growth [10,11].…”

mentioning

confidence: 99%

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

View full text Add to dashboard Cite

In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C 1 + C 2 × e (−X/C 3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C 1−3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.

show abstract

Impedance based sensing of the specific binding reaction between Staphylococcus enterotoxin B and its antibody on an ultra-thin platinum film

Cited by 90 publications

References 6 publications

Label‐Free Impedance Biosensors: Opportunities and Challenges

Label‐Free Impedance Biosensors: Opportunities and Challenges

Electrochemical sensors: a powerful tool in analytical chemistry

Development of a novel conductance-based technology for environmental bacterial sensing

Contact Info

Product

Resources

About