Impedance Based Nanotoxicity Assessment of Graphene Nanomaterials at the Cellular and Tissue Level

Hondroulis, Evangelia; Zhang, Zhiqi; Chen, Chunying; Li, Chen-Zhong

doi:10.1080/00032719.2011.633184

Cited by 33 publications

(15 citation statements)

References 29 publications

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“…The resistance values measured after graphene was incorporated with the cells showed little difference compared with the control, indicating that graphene does not interfere with cellular attachment. In conclusion, this study highlights the biocompatibility of graphene and the broad potential of using these nanomaterials for biomedical applications [159].…”

Section: Blood-brain Barrier Overlap By Graphene Nanomaterialsmentioning

confidence: 98%

Graphene-Based Nanomaterials: Biological and Medical Applications and Toxicity

Tonelli

Goulart

Gomes

et al. 2015

Nanomedicine (Lond.)

165

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Graphene and its derivatives, due to a wide range of unique properties that they possess, can be used as starting material for the synthesis of useful nanocomplexes for innovative therapeutic strategies and biodiagnostics. Here, we summarize the latest progress in graphene and its derivatives and their potential applications for drug delivery, gene delivery, biosensor and tissue engineering. A simple comparison with carbon nanotubes uses in biomedicine is also presented. We also discuss their in vitro and in vivo toxicity and biocompatibility in three different life kingdoms (bacterial, mammalian and plant cells). All aspects of how graphene is internalized after in vivo administration or in vitro cell exposure were brought about, and explain how blood-brain barrier can be overlapped by graphene nanomaterials.

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Section: Blood-brain Barrier Overlap By Graphene Nanomaterialsmentioning

confidence: 98%

Graphene-Based Nanomaterials: Biological and Medical Applications and Toxicity

Tonelli

Goulart

Gomes

et al. 2015

Nanomedicine (Lond.)

165

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show abstract

“…In comparison to nanoparticles of similar hydrodynamic size, graphene has a very large potential for lipid interaction due to its extended 2D surface (Nel et al, 2006). In addition to physicochemical properties of the environment, graphene purity, diameter, length, surface charge, functionalization and aggregation state can influence the toxicity (Long et al, 2012;Hondroulis et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The effects of graphene oxide on green algae Raphidocelis subcapitata

2015

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“…As such, astrocytes growth resistance/impedance was measured for 10 hours post-treatment using the electric cell-substrate impedance sensing method. This method measures the resistance (Ω) produced by growing cell monolayers over the electrodes and can detect changes in resistance to AC current flow that may occur with changes in the cell layer 47 48 . Primarily, growth resistance of each treatment group is compared with resistance of blank well containing culture media to obtain blank-normalized resistance value.…”

Section: Resultsmentioning

confidence: 99%

“…A little up or down slope of the growth kinetics among groups is expected during the early hour due to variations in the cell attachment rate which can be influenced by many other factors such as initial cell density, cellular transitory metabolic slowdown due to treatment effects, etc. 47 48 . Thus, a longer monitoring of cell growth-behavent, impedance sensing method may reflect a healthy cellular status and factors such as cell attachment and metabolic pause can be nullified.…”

Section: Resultsmentioning

confidence: 99%

“…Astrocytes growth resistance/impedance was measured with the help of the Electric cell-substrate impedance sensing instrument (model 1600RE, Applied Biophysics, USA) using 8W10E PET chips (Applied Biophysics), containing 8 cell culture wells 47 48 . Each well of the chip contains 10 working electrodes (250 μm diameter) embedded in parallel on a gold connection pad and all wells share a common reference electrode.…”

Section: Methodsmentioning

confidence: 99%

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Coupling of transient near infrared photonic with magnetic nanoparticle for potential dissipation-free biomedical application in brain

Sagar

Atluri

Tomitaka

et al. 2016

Sci Rep

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Combined treatment strategies based on magnetic nanoparticles (MNPs) with near infrared ray (NIR) biophotonic possess tremendous potential for non-invasive therapeutic approach. Nonetheless, investigations in this direction have been limited to peripheral body region and little is known about the potential biomedical application of this approach for brain. Here we report that transient NIR exposure is dissipation-free and has no adverse effect on the viability and plasticity of major brain cells in the presence or absence superparamagnetic nanoparticles. The 808 nm NIR laser module with thermocouple was employed for functional studies upon NIR exposure to brain cells. Magnetic nanoparticles were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic laser scattering (DLS), and vibrating sample magnetometer (VSM). Brain cells viability and plasticity were analyzed using electric cell-substrate impedance sensing system, cytotoxicity evaluation, and confocal microscopy. When efficacious non-invasive photobiomodulation and neuro-therapeutical targeting and monitoring to brain remain a formidable task, the discovery of this dissipation-free, transient NIR photonic approach for brain cells possesses remarkable potential to add new dimension.

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Impedance Based Nanotoxicity Assessment of Graphene Nanomaterials at the Cellular and Tissue Level

Cited by 33 publications

References 29 publications

Graphene-Based Nanomaterials: Biological and Medical Applications and Toxicity

Graphene-Based Nanomaterials: Biological and Medical Applications and Toxicity

The effects of graphene oxide on green algae Raphidocelis subcapitata

Coupling of transient near infrared photonic with magnetic nanoparticle for potential dissipation-free biomedical application in brain

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