Impairment of thymus development in cultured osmerid fish, the ayu, Plecoglossus altivelis

Miwa, Satoshi; Sakai, Akihisa; Nakane, Motoyuki

doi:10.1016/s0044-8486(02)00661-0

Cited by 8 publications

(12 citation statements)

References 15 publications

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“…Estimation of thymus development Miwa et al (2003) showed a clear positive correlation between thymus volume and body length in wild ayu that were sampled before the day length started to shorten. To eliminate errors associated with fish size, we evaluated thymus development using the ratio of thymus volume to body length.…”

Section: Tissue Sectioning and Measurement Of Thymus Volumementioning

confidence: 95%

“…Transverse sections were cut through the tissue every 200 μm and stained with hematoxylin and eosin (H & E). The cross-sectional area of thymus tissue within each section was measured using ImageJ software (https://imagej.nih.gov/ij/), and the total volume of each thymus was estimated as follows (Miwa et al, 2003):…”

Section: Tissue Sectioning and Measurement Of Thymus Volumementioning

confidence: 99%

“…Therefore, the thymus probably plays an important role in the development of immune responses associated with T-cell function. Miwa et al (2003) reported that development of the thymus of ayu is affected by rearing water temperature during the juvenile stage for 3 months from about 0.5 g body weight. The thymus was significantly smaller in juvenile ayu reared at higher water temperature (17°C-18°C) than in those reared at lower water temperatures (13°C-14°C).…”

mentioning

confidence: 99%

“…Measurement of thymus volume is necessary for studying thymus development. To date, thymus volume have been estimated by using serial histological sections (Miwa et al, 2003). However, this method is laborious and time-consuming and is unsuitable for analysis of large numbers of samples.…”

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confidence: 99%

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Subsequent Changes in the Thymus of Ayu <i>Plecoglossus altivelis</i> Reared from Juveniles with Temperature-induced Differential Thymus Growth

et al. 2020

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The temperature of water used to rear juvenile stages of ayu affects the development of the thymus. However, subsequent differences in the thymus in adults after rearing juveniles at different temperatures are unclear. We analyzed thymus development of ayu grown at a constant water temperature of 15°C from juveniles reared at different water temperatures. Juvenile ayu weighed at 0.6 g and 1.4 g were reared at different temperatures (10°C, 12°C, 15°C, 18°C and 22°C) for 64 days and 54 days, respectively, and then held at 15°C for analysis of the thymus development. Thymus volume was measured by computed tomography and thymus development of experimental fish was evaluated as the ratio of thymus volume to body length. The thymus volume ratios of juveniles immediately after rearing at lower water temperatures (10°C, 12°C) were higher than in those reared at the higher temperatures (18°C, 22°C). These levels of thymus volume ratio were preserved in grown ayu continuously reared at 15°C. After the summer solstice, the thymus began to atrophy and no longer exhibited differences among the experimental groups. Thus the thymus formed at the juvenile stage retains its morphological properties until the summer solstice.

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Section: Tissue Sectioning and Measurement Of Thymus Volumementioning

confidence: 95%

Section: Tissue Sectioning and Measurement Of Thymus Volumementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Subsequent Changes in the Thymus of Ayu <i>Plecoglossus altivelis</i> Reared from Juveniles with Temperature-induced Differential Thymus Growth

et al. 2020

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“…Season-dependent fluctuations in the number of peripheral lymphocytes and morphological changes in lymphoid tissues have been observed in different teleost species [ 106 , 107 , 108 , 109 ]; interestingly, the thymus is one of the lymphoid organs most affected by seasonal changes. In winter, while natural daylight decreases, thymus involution became evident in Ayu sweetfish ( Plecoglossus altivelis ) [ 109 ]. The dimensions of the organ were measured to establish its initial size in summertime, and histological techniques were used to evaluate the involution of the tissue; a reduction of the epithelial thickness of the organ was observed, when compared with the size during the rest of the year.…”

Section: Factors Modulating Thymus Development and Presence In Fismentioning

confidence: 99%

Revisiting the Teleost Thymus: Current Knowledge and Future Perspectives

Barraza

Montero

Wong-Benito

et al. 2020

Biology

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The thymus in vertebrates plays a critical role in producing functionally competent T-lymphocytes. Phylogenetically, the thymus emerges early during evolution in jawed cartilaginous fish, and it is usually a bilateral organ placed subcutaneously at the dorsal commissure of the operculum. In this review, we summarize the current understanding of the thymus localization, histology studies, cell composition, and function in teleost fishes. Furthermore, we consider environmental factors that affect thymus development, such as seasonal changes, photoperiod, water temperature fluctuations and hormones. Further analysis of the thymus cell distribution and function will help us understand how key stages for developing functional T cells occur in fish, and how thymus dynamics can be modulated by external factors like photoperiod. Overall, the information presented here helps identify the knowledge gaps and future steps needed for a better understanding of the immunobiology of fish thymus.

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Effects of suspended sediment on cortisol levels in wild and cultured strains of ayu Plecoglossus altivelis

et al. 2011

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Impairment of thymus development in cultured osmerid fish, the ayu, Plecoglossus altivelis

Cited by 8 publications

References 15 publications

Subsequent Changes in the Thymus of Ayu <i>Plecoglossus altivelis</i> Reared from Juveniles with Temperature-induced Differential Thymus Growth

Subsequent Changes in the Thymus of Ayu <i>Plecoglossus altivelis</i> Reared from Juveniles with Temperature-induced Differential Thymus Growth

Revisiting the Teleost Thymus: Current Knowledge and Future Perspectives

Effects of suspended sediment on cortisol levels in wild and cultured strains of ayu Plecoglossus altivelis

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