Impairment of splenic natural killer cell activity of mice infected with the polycythemic strain of friend leukemia virus

Migliorati, Graziella; Jezzi, Teresa; Favalli, Cartesio; Garaci, Enrico; Rossi, Giovanni Battista; Bonmassar, Enzo

doi:10.1007/bf00205378

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Table I illustrates the results of a typical experiment in which splenic NK cell activity of DBN2 mice infected with FLV-P 21 days before assay is compared to that of untreated controls. These results confirm our previous observations in that such infection resulted in a significant decrease of host NK activity (Migliorati et al, 1982). Since at this time a marked splenomegaly can be found in infected mice we also considered the number of spleen cells in relationship to the NK effector function.…”

Section: Effect Of Flv-p Infection On Splenic N K Activity Of Dbai2 Micesupporting

confidence: 90%

“…We have previously reported that 14-21 days after FLV-P infection of susceptible DBN2 mice a signifi- IxT ratio cant decrease of NK cell activity could be detected in host splenocytes (Migliorati et al, 1982). Table I illustrates the results of a typical experiment in which splenic NK cell activity of DBN2 mice infected with FLV-P 21 days before assay is compared to that of untreated controls.…”

Section: Effect Of Flv-p Infection On Splenic N K Activity Of Dbai2 Micementioning

confidence: 99%

“…The virus-induced experimental leukemia is widely studied as a model for the oncogenic process and immunosuppression associated with neoplasia (Salaman and Wedderburn, 1966;Friedman and Ceglowski, 1971;Mortensen et al, 1973Mortensen et al, , 1974Garaci et al, 1981). In the light of the possible in vivo role of natural reactivity and following the suggestion that this activity can be involved in the complex antitumor surveillance mechanisms (Haller et al, 1977;Herberman and Holden, 1978), we have studies performed concerning the influence of ELV infection on NK activity in virus-susceptible mice (Migliorati et al, 1982). In particular, the investigations have been conducted with mice bearing the polycythemic substrain of FLV, i.e., FLV-P virus which is able to induce a rapid non-thymic erythroid leukemia in susceptible recipients and produces splenomegaly and polycythemia leading to early death of the host (Mirand et al, 1968).…”

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Modulation of natural killer (NK) cell activity during FLV‐P virus infection of mice

Migliorati

Jezzi²,

Frati

et al. 1983

Intl Journal of Cancer

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We analyzed the effects of a polycythemic substrain of Friend leukemia virus, i.e. the FLV-P virus, on splenic NK activity of DBA/2 susceptible mice. One day after virus injection a significant increase of NK activity was found, which persisted until day 10. On the other hand, 14-21 days after virus injection a marked and significant depression of activity was measured. This depression was associated with the appearance of suppressor cells able to inhibit the lytic activity of untreated splenocytes when mixed in vitro in the 4 h 51Cr-release assay. The suppressor cell population was insensitive to treatment with anti-Thy 1.2 plus complement, was adherent to Sephadex G-10 and nylon, but did not adhere to plastic, suggesting it is neither a T-cell nor a typical macrophage. The possible relevance of NK activity modulation in relation to the induction of leukemia is discussed.

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Section: Effect Of Flv-p Infection On Splenic N K Activity Of Dbai2 Micesupporting

confidence: 90%

Section: Effect Of Flv-p Infection On Splenic N K Activity Of Dbai2 Micementioning

confidence: 99%

mentioning

confidence: 99%

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Modulation of natural killer (NK) cell activity during FLV‐P virus infection of mice

Migliorati

Jezzi²,

Frati

et al. 1983

Intl Journal of Cancer

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Immunotherapy of murine leukemia. XI. Differential susceptibility of spleen cells from serum‐protected mice to the in vitro immunosuppressive effects of friend leukemia virus‐infected splenocytes

Pettey

Collins

1984

Intl Journal of Cancer

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It has previously been shown that DBA/2 mice protected against the development of Friend leukemia virus (FLV)4-induced disease by the passive administration of heterologous antisera directed against disrupted virions or the major viral envelope glycoprotein (gp71) fail to undergo the generalized immunosuppression which characterizes FLV leukemogenesis. In the present studies, the susceptibility of spleen cells from serum-protected mice to the immunosuppressive effects of FLV-infected spleen cells has been examined by means of in vitro assays of antibody production and of natural killing (NK). In contrast to the parallel suppression of both functions in FLV-infected mice and their lack of suppression in serum-protected animals, a dichotomy was observed in the in vitro susceptibility of these activities of spleen cells from serum-protected mice to the suppressive effects of virus-infected splenocytes, implying that more than one mechanism of suppression is operative. Thus, the antibody-producing capability of serum-protected splenocytes was not suppressed by FLV-infected spleen cells, this suppression reflecting the activity of infectious virus, while the NK effector function of serum-protected spleen cells was as susceptible as that of normal splenocytes to suppression by virus-infected spleen cells. This suppression of NK activity is mediated by virus-induced suppressor cells and does not involve free infectious virus, in contrast to suppression of antibody production. These results thus indicate that, while serum therapy inhibits the development of the virus-induced NK suppressor cells found in the spleens of progressively infected mice, splenic NK effectors, which are present, demonstrate full susceptibility to the suppressive effects of such independently generated virus-induced suppressor cells. Preliminary attempts at characterizing these NK suppressors in the spleens of FLV-infected mice indicate that they are not eliminated by treatment with antisera directed against T cells, B cells, macrophages or FLV gp71. However, Percoll fractionation of spleen cells from infected mice has revealed the presence of an expanded (as compared to normal spleen cells) population of cells of density 1.077 which demonstrates high in vitro NK suppressor activity, presumably representing the virus-induced suppressor cells measured in this assay. At the present time, the mechanism of the resistance of serum-protected spleen cells' antibody production to suppression by FLV-infected splenocytes (i.e., by FLV itself) remains undefined.

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Influence of Vindesine on the Lytic Phase of Mouse Natural Cytotoxicity Against Human Leukemic Cells

Pastore

Vecchis

Migliorati

et al. 1982

Journal of Immunopharmacology

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Vindesine (VDS), a structural analogue of Vinca Alkaloids, was found to increase the NK-mediated cytolytic effects of mouse lymphocytes against human K562 target cells in a 18-hr assay. Pretreatment of effector or target cells with the drug did not affect substantially the NK reaction. The phenomenon has been detected using splenocytes of either congenitally athymic or conventional euthymic mice of different strains. Effector lymphocytes deprived of cells adherent to plastic surface or to nylon-wool column were still competent for drug-mediated increase of NK function. It is suggested that modification of the membrane make-up of effector or target cells reversibly induced by VDS, would promote higher NK-mediated cytolytic effects.

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Impairment of splenic natural killer cell activity of mice infected with the polycythemic strain of friend leukemia virus

Cited by 4 publications

References 16 publications

Modulation of natural killer (NK) cell activity during FLV‐P virus infection of mice

Modulation of natural killer (NK) cell activity during FLV‐P virus infection of mice

Immunotherapy of murine leukemia. XI. Differential susceptibility of spleen cells from serum‐protected mice to the in vitro immunosuppressive effects of friend leukemia virus‐infected splenocytes

Influence of Vindesine on the Lytic Phase of Mouse Natural Cytotoxicity Against Human Leukemic Cells

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