Impairment of renal steroidogenesis at the onset of diabetes

Pagotto, Melina A.; Roldán, María Lorena; Molinas, Sara M.; Raices, Trinidad; Pisani, G; Pignataro, Omar Pedro; Monasterolo, Liliana A.

doi:10.1016/j.mce.2021.111170

Cited by 4 publications

(6 citation statements)

References 51 publications

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“…The study showed higher expression levels of TSPO in HG-induced HK2 cells or kidneys of the diabetic mice, suggesting that TSPO can be used as a biomarker for DKD. However, this result differs from the findings of Pagotto et al that show the expression level of TSPO in the rat kidney after streptozotocin-only treatment was upregulated, but there was no significant difference with the healthy kidney . This may be related to the different ways of DKD induction.…”

Section: Discussioncontrasting

confidence: 99%

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Adropin Carried by Reactive Oxygen Species-Responsive Nanocapsules Ameliorates Renal Lipid Toxicity in Diabetic Mice

Zhong

et al. 2022

ACS Appl. Mater. Interfaces

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Diabetic kidney disease (DKD) is a common diabetes complication mainly caused by lipid toxicity characterized by oxidative stress. Studies have shown that adropin (Ad) regulates energy metabolism and may be an effective target to improve DKD. This study investigated the effect of exogenous Ad encapsulated in reactive oxygen species (ROS)-responsive nanocapsules (Ad@Gel) on DKD. HK2 cells were induced with high glucose (HG) and intervened with Ad@Gel. A diabetes mouse model was established using HG and high-fat diet combined with streptozotocin and treated with Ad@Gel to observe its effects on renal function, pathological damage, lipid metabolism, and oxidative stress. Results showed that Ad@Gel could protect HK2 from HG stimulation in vitro. It also effectively controls blood glucose and lipid levels, improves renal function, inhibits excessive production of ROS, protects mitochondria from damage, improves lipid deposition in renal tissues, and downregulates the expression of lipogenic proteins SEBP-1 and ADRP in DKD mice. In HG-induced HK2 cells or the kidney of DKD patients, the low expression of neuronatin (Nnat) and high expression of translocator protein (TSPO) were observed. Knockdown Nnat or overexpression of TSPO significantly reversed the effect of Ad@Gel on improving mitochondrial damage. In addition, knockdown Nnat also significantly reversed the effect of Ad@Gel on lipid metabolism. The results suggest that the effect of Ad on DKD may be achieved by activating Nnat to improve lipid metabolism and inhibit TSPO activity, thereby enhancing mitochondrial function.

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Section: Discussioncontrasting

confidence: 99%

“…However, this result differs from the findings of Pagotto et al that show the expression level of TSPO in the rat kidney after streptozotocin-only treatment was upregulated, but there was no significant difference with the healthy kidney. 50 This may be related to the different ways of DKD induction.…”

Section: ■ Discussionmentioning

confidence: 99%

Adropin Carried by Reactive Oxygen Species-Responsive Nanocapsules Ameliorates Renal Lipid Toxicity in Diabetic Mice

Zhong

et al. 2022

ACS Appl. Mater. Interfaces

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“…Pagotto et al . 19 confirmed through animal experiments that some mechanisms leading to the occurrence and progression of DN have been triggered, such as inflammation, fibrosis and the expression of stress response mediators, before kidney damage occurs in the early stage of diabetes 4 , 15 . As a common steroid hormone, the level of DHEA decreases with age 1 , 9 , 20 .…”

Section: Discussionmentioning

confidence: 83%

“…A high‐glucose environment leads to vascular dysfunction and induces DN, including some heterogeneous pathological mechanism drivers, such as oxidative stress, inflammation and fibrosis 19 . Pagotto et al 19 .…”

Section: Discussionmentioning

confidence: 99%

“…A high-glucose environment leads to vascular dysfunction and induces DN, including some heterogeneous pathological mechanism drivers, such as oxidative stress, inflammation and fibrosis 19 . Pagotto et al 19 confirmed through animal experiments that some mechanisms leading to the occurrence and progression of DN have been triggered, such as inflammation, fibrosis and the expression of stress response mediators, before kidney damage occurs in the early stage of diabetes 4,15 . As a common steroid hormone, the level of DHEA decreases with age 1,9,20 .…”

Section: Discussionmentioning

confidence: 99%

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