Impairment of Phytohaemagglutinin-Induced Blastic Transformation in Lymphnodes from Thymectomized Mice

Dukor, P.; Dietrich, Felix M.

doi:10.1159/000229964

Cited by 38 publications

(13 citation statements)

References 32 publications

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“…On the assumption, as is generally accepted, that the mixed leukocyte response is representative of cellular immunity, the findings would support the currently accepted view that the cells mediating cellular immunity are thymic or thymus-derived and that their response to PHA is indicative of this immunologic function. It has been reported that the response of lymphocytes of neonatally thymectomized mice (63), rats (64), and chickens (65) to stimulation with PHA or mitomycin C-treated aUogeneic cells is markedly reduced. Furthermore, it has been demonstrated that lymphocytes obtained from thymectomized rats are incapable of responding to stimulation with allogeneic lymphocytes in the mixed leukocyte system (57).…”

Section: Discussionmentioning

confidence: 99%

Cells Involved in the Immune Response

Daguillard

Richter

1969

The Journal of Experimental Medicine

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Cells of the different lymphoid organs in the normal adult rabbit were investigated for their capacity to respond in vitro to a number of stimuli, such as phytohemagglutinin (PHA), anti-rabbit immunoglobulin antiserum (GARIG) and allogeneic and xenogeneic lymphoid cells, and for their capacity to adsorb radioactively-labeled anti-immunoglobulin antiserum. The bone marrow cells responded minimally to PHA, GARIG, and the allogeneic and xenogeneic stimuli. The thymus cells were unable to respond to stimulation with GARIG although they responded to the other stimuli. The cells of the other lymphoid organs tested responded to all the mitogenic agents, to varying degrees. On the basis of the results presented and the findings of other investigators, it is concluded that: 1. The response of the cells to GARIG indicates a potential capacity to mediate humoral immunity and requires the presence of immunoglobulin or immunoglobulin-like recognition sites on the cell surface. 2. The response of the cells to PHA and allogeneic and xenogeneic cells indicates a potential capacity to mediate cellular immunity and does not necessitate the presence of immunoglobulin-recognition sites on the cell surface. 3. The thymus in the normal adult rabbit consists of cells capable of mediating cellular immunity only. 4. The other lymphoid organs appear to possess cells capable of mediating humoral and cellular immunity.

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Section: Discussionmentioning

confidence: 99%

Cells Involved in the Immune Response

Daguillard

Richter

1969

The Journal of Experimental Medicine

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“…The heterogeneity of splenic mononuclear cells has been demonstrated by the work of P lo tz and T a la l [20], who were able to fractionate splenic cells by passage over glass bead columns. It has also been demonstrated that both the spleen and lymph node possess thymus-dependent and thymus-independent lymphoid cells [21][22][23] and that thymectomy can abolish the reactivity of the lymphatic tissue to PHA [24]. It is obvious, therefore, that lymphoid cell preparations of each of these two organs are themselves functionally heterogeneous.…”

Section: Resultsmentioning

confidence: 99%

Cells Involved in the Immune Response

Singhal¹,

Daguillard²,

Richter³

1968

Int Arch Allergy Immunol

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“…As indicated earlier, there is suggestive evidence from experimental animal studies, as well as observations in patients with immunodeficient diseases, to consider PHA responsiveness a function of the T-lymphocyte system, and PWM responsiveness a measure of the B-lymphocyte system (although not exclusively) (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)53). Our data may indicate that in HD a depletion of circulating T-lymphocytes occurs, probably in all stages of disease, but most evident in advanced HD, while B-lymphocytes appear to be present in relatively normal amounts.…”

Section: Methodsmentioning

confidence: 95%

“…Although not yet settled, it appears that phytohemagglutinin (PHA) stimulates T-lymphocytes predominantly, if not exclusively (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35), whereas pokeweed mitogen (PWM) may stimulate both B-and T-lymphocytes (32,33). There is, however, some data to suggest that the B-lymphocyte response to PWM may be greater than that of the T-lymphocyte (31,33,35).…”

Section: Introductionmentioning

confidence: 99%