Impairment of peroxisomal APX and CAT activities increases protection of photosynthesis under oxidative stress

Sousa, Rachel H. V.; Carvalho, Fernanda Torres de; Lima‐Melo, Yugo; Alencar, Vicente T.C.B.; Daloso, Danilo de Menezes; Margis‐Pinheiro, Márcia; Komatsu, Setsuko; Silveira, Joaquim Albenísio Gomes da

doi:10.1093/jxb/ery354

Cited by 40 publications

(17 citation statements)

References 48 publications

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“…However, peroxisomes, which share a number of metabolites and enzymes common to all types of peroxisome, is now the preferred term regardless of their specific metabolic characteristics (Pracharoenwattana and Smith, 2008). The most noteworthy metabolites and enzymes include H 2 O 2 and catalase, which are directly involved in the metabolism of reactive oxygen species (ROS) (Su et al, 2018;Sousa et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Plant Peroxisomes: A Factory of Reactive Species

2020

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Plant peroxisomes are organelles enclosed by a single membrane whose biochemical composition has the capacity to adapt depending on the plant tissue, developmental stage, as well as internal and external cellular stimuli. Apart from the peroxisomal metabolism of reactive oxygen species (ROS), discovered several decades ago, new molecules with signaling potential, including nitric oxide (NO) and hydrogen sulfide (H 2 S), have been detected in these organelles in recent years. These molecules generate a family of derived molecules, called reactive nitrogen species (RNS) and reactive sulfur species (RSS), whose peroxisomal metabolism is autoregulated through posttranslational modifications (PTMs) such as S-nitrosation, nitration and persulfidation. The peroxisomal metabolism of these reactive species, which can be weaponized against pathogens, is susceptible to modification in response to external stimuli. This review aims to provide up-to-date information on crosstalk between these reactive species families and peroxisomes, as well as on their cellular environment in light of the well-recognized signaling properties of H 2 O 2 , NO and H 2 S.

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Section: Introductionmentioning

confidence: 99%

Plant Peroxisomes: A Factory of Reactive Species

2020

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“…The enzyme CAT has a higher occurrence in peroxisomes due to the photorespiratory process, and APX occurs mainly in chloroplasts [ 72 ]. Due to the higher H 2 O 2 formation as a consequence of photorespiration, higher CAT activity may be expected in environments with higher photorespiratory rates, in this case in FS, and higher APX activity under low irradiance (DS), where H 2 O 2 is formed in a smaller amount [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

“…The modification of the balance of enzyme activity in stressful situations can lead to compensatory mechanisms: suppressing the activity of one enzyme might induce the synthesis of another with the same purpose [ 74 ]. Although the role of APX in the elimination of H 2 O 2 is recognized, CAT is considered more effective, which makes their greater activity in FS necessary [ 73 ]. Despite the lower CAT activity of pioneer species, B. grossularioides and O. pyramidale had higher proportional activity of CAT in relation to APX in FS (17 and four times, respectively).…”

Section: Discussionmentioning

confidence: 99%

Photochemical Efficiency and Oxidative Metabolism of Tree Species during Acclimation to High and Low Irradiance

Junior

Maia

Martins

et al. 2020

Plants

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The balance between efficiency of absorption and use of light energy is fundamental for plant metabolism and to avoid photoinhibition. Here, we investigated the effects of light environments on the photosynthetic apparatus of tropical tree species of three successional groups (pioneer, mid-, and late successional) subjected to different light conditions: full sunlight (FS), moderate shade (MS), and deep shade (DS). Twenty-nine ecophysiological parameters were correlated with each other. The pioneer species exhibited better photochemical performance and a more efficient antioxidant enzymatic system in comparison with the other successional groups. Plants in FS showed higher intensity of lipid peroxidation, with superoxide dismutase having a prominent role in the antioxidant system. At lower irradiance the enzymatic activity was reduced, and the photochemical efficiency was the preferred way to reduce oxidative damages. P was highly related to photochemical yield, and the N modulation amplified the light harvesting complex in DS to the detriment of the antioxidant system. Despite evidence of cell damage, most species exhibited the ability to adjust to high irradiance. Contrary to expectations, Hymenea courbaril (late-successional) exhibited higher plasticity to fluorescence, nutritional, and antioxidant parameters. Only Carapa guianensis (late-successional) displayed photoinhibitory damage in FS, and Ochroma pyramidale (pioneer) did not survive in DS, suggesting that acclimation to shade is more challenging than to high irradiance.

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“…CatC is an important antioxidant and key enzyme in the defence system established by plants during growth. CatC can inhibit the excessive growth of H 2 O 2 , and is thus an important enzyme in the plant that controls H 2 O 2 levels and the redox balance of plant cells [27]. CatC plays an important role in stress resistance, and it can improve the defence ability of rice [28].…”

Section: Discussionmentioning

confidence: 99%

Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics

et al. 2019

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This work was designated to scrutinize the protein differential expression in natural selenium-enriched and non-selenium-enriched rice using the Isobaric-tags for relative and absolute quantification (iTRAQ) proteomics approach. The extracted proteins were subjected to enzyme digestion, desalting, and identified by iTRAQ coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. High pH C18 separation analysis was performed, and the data were then analyzed by Protein PilotTM (V4.5) search engine. Protein differential expression was searched out by comparing relatively quantified proteins. The analysis was conducted using gene ontology (GO), cluster of orthologous groups of proteins (COG) and Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathways. A total of 3235 proteins were detected and 3161 proteins were quantified, of which 401 were differential proteins. 208 down-regulated and 193 up-regulated proteins were unveiled. 77 targeted significant differentially expressed proteins were screened out for further analysis, and were classified into 10 categories: oxidoreductases, transferases, isomerases, heat shock proteins, lyases, hydrolases, ligases, synthetases, tubulin, and actin. The results indicated that the anti-stress, anti-oxidation, active oxygen metabolism, carbohydrate and amino acid metabolism of natural selenium-enriched rice was higher than that of non-selenium rice. The activation of the starch synthesis pathway was found to be bounteous in non-selenium-enriched rice. Cysteine synthase (CYS) and methyltransferase (metE) might be the two key proteins that cause amino acid differences. OsAPx02, CatC, riPHGPX, HSP70 and HSP90 might be the key enzymes regulating antioxidant and anti-stress effect differences in two types of rice. This study provides basic information about deviations in protein mechanism and secondary metabolites in selenium-enriched and non-selenium-enriched rice.

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Impairment of peroxisomal APX and CAT activities increases protection of photosynthesis under oxidative stress

Abstract: Proteomics and physiological approaches reveal that peroxisomal APX silencing and CAT inhibition trigger H 2 O 2 -signalling for up-regulation of antioxidant proteins to protect the rice photosynthetic apparatus under oxidative stresss.

Cited by 40 publications

References 48 publications

Plant Peroxisomes: A Factory of Reactive Species

Plant Peroxisomes: A Factory of Reactive Species

Photochemical Efficiency and Oxidative Metabolism of Tree Species during Acclimation to High and Low Irradiance

Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics

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