Impairment of liver regeneration correlates with activated hepatic NKT cells in HBV transgenic mice

Dong, Chen; Zhang, Jianhong; Sun, Rui; Wei, Haiming; Tian, Zhigang

doi:10.1002/hep.21597

Cited by 59 publications

(66 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is conflicting evidence on the proliferation of HBV infected hepatocytes [25], with data suggesting both inhibition [26,27] and induction of proliferation [28]. However, HBV infection is indeed observed to persist across cell divisions, as infected cells often contain multiple copies of the viral genome [29].…”

Section: Model With Infected Cell Proliferationmentioning

confidence: 99%

Mathematical models of the interrelated dynamics of hepatitis D and B

Packer

Forde

Hews

et al. 2014

Mathematical Biosciences

View full text Add to dashboard Cite

Section: Model With Infected Cell Proliferationmentioning

confidence: 99%

Mathematical models of the interrelated dynamics of hepatitis D and B

Packer

Forde

Hews

et al. 2014

Mathematical Biosciences

View full text Add to dashboard Cite

“…13 Splenic single-cell leukocyte suspensions were prepared by lysing erythrocytes with 103 red blood cell lysis buffer (Biolegend, CA, USA). Single-cell suspensions of LNs were prepared by pressing the LNs through mesh.…”

Section: Hydrodynamic Transfection Of Mice With Plasmidsmentioning

confidence: 99%

Characterization of the liver-draining lymph nodes in mice and their role in mounting regional immunity to HBV

Zheng

Tian

2013

Cell Mol Immunol

Self Cite

View full text Add to dashboard Cite

The lymphatic system is important in mounting an immune response to foreign antigens and tumors in humans and animal models. The liver produces a large amount of lymph, and its lymphatic system is divided into three major components: the portal, sublobular and superficial lymphatic vessels. Despite the fact that mice are the most commonly used laboratory animals, detailed descriptions of the anatomical location and function of the lymph nodes (LNs) that drain the liver are surprisingly absent. In this study, we found that the portal and celiac LNs adjacent to mouse liver were stained with Evans blue within 5-8 min. Enhanced green fluorescence protein (EGFP)-positive cells from the liver also drained into the two aforementioned LNs. These data indicate that the portal and celiac LNs drain the mouse liver. Lymphadenectomy of the identified liver-draining LNs resulted in hepatitis B virus (HBV) persistence in immunocompetent mice compared with the sham group. In addition, the frequencies of CD81 T cells and dendritic cells (DCs) increased significantly in the liver-draining LNs after hydrodynamic injection of HBV plasmid. Liver-draining LN cells in HBV plasmid-injected mice also showed significant antigen-specific proliferation in response to stimulation with recombinant hepatitis B core antigen in vitro. Adoptive transfer of these cells into Rag1 2/2 mice induced a reduction in the serum concentration of hepatitis B surface antigen (HBsAg) compared to liver-draining LN cells in uninjected mice. Altogether our data characterize the liver-draining LNs and provide evidence that the liver-draining LNs induce an anti-HBV-specific immune response responsible for HBV clearance.

show abstract

“…HMCs were isolated by metrizamide gradient centrifugation as reported (24). Briefly, the livers were minced, pressed through a 200-gauge stainless steel mesh.…”

Section: Preparation Of Hepatic Mononuclear Cells (Hmcs)mentioning

confidence: 99%