Impairment of bone growth of wistar rat fetuses of diabetic and hypercholesterolemic mothers

Maternal alloxan exposure induces damage in rat offspring lumbar vertebrae and protective role of arachidonic acid

“…The pericellular spaces and lacunae were widened with sparse collagen fibrils in the ECM as compared with the control group. These findings agreed with other researchers who have found that the hindlimb bones of the embryos of the diabetic mothers exhibited malformed chondrocytes, which possessed vacuolated cytoplasm, massive breakdown of their cytoplasmic compartments, and clumping of their nuclear chromatin indicating apoptosis [36].…”

“…In contrast, the alloxan-induced diabetes group of the present study showed markedly diminished staining of the matrix between the growing chondrocytes of the epiphyseal growth plates. These results supported previous studies that reported that hyperglycemia can lead to bone loss, a decrease in bone density, and bone microarchitecture impairment in rats [36,40]. Also, hyperglycemia changes the mineral composition and collagen integrity of bones, causing the marrow cavity to become full of fat and adipogenic mesenchymal stem cells [41,42].…”

“…In harmony, another group of researchers showed decrease in thickness of the epiphyseal growth plates of the femur of the diabetic rats [35]. Also, similar results were obtained by other investigators who observed that maternal diabetes delayed the bone ossification in rat fetuses [7,36]. They also found that the growing chondrocytes were disorganized, distorted, small-sized, and with vacuolated cytoplasm.…”

Effects of Maternal Diabetes on Prenatal Development of The Vertebral Column in The Albino Rat and Possible Protective Role of Arachidonic Acid

“…The surrounding pericellular space appears widened. Similar findings were observed by El-Sayyad et al (2015) who found that the chondrocytes in the hindlimb bones of the diabetic mothers' embryos were deformed, with vacuolated cytoplasm, severe cytoplasmic compartment disruption, and clumping of their nuclear chromatin, which indicated apoptosis. King (2012) revealed that Alloxan's diabetic effect is mostly linked to its quick uptake by beta cells, which increases the creation of free radicals and reactive oxygen species.…”