Impairing effect of fibrinogen on the mono-/bi-layer form of bovine lung surfactant

Devraj, Ravi; Nag, Kaushik; Nahak, Prasant; Manna, Kausik; Fritzen‐Garcia, Mauricia; Thompson, David W.; Makino, Kimiko; Ohshima, Hiroyuki; Nakahara, Hiromichi; Shibata, Osamu; Panda, Amiya Kumar

doi:10.1007/s00396-014-3319-4

Cited by 3 publications

(15 citation statements)

References 31 publications

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“…The NaCl-Trizma ® . HCl buffer used in this study was prepared by the addition of 150 mM NaCl and 5 mM 0.08 g Trizma hydrochloride in 1 litre of double distilled water and the pH was adjusted to 7 by titrating with 0.1M NaOH 15 . Each sample was prepared by incubating desired amount 10 and 20 wt of FCS, cholesterol and LDL with appropriate amounts of BLES followed by thorough mixing.…”

Section: Methodsmentioning

confidence: 99%

“…Sample dispersions were injected thorough a rubber valve into a cylindrical teflon cup of 5 mL capacity and a surface area of 6.28 sq. cm 15 25 27 . Samples were imaged within two hours from the films being deposited to prevent dehydration 17 .…”

Section: Adsorption Kinetics Studiesmentioning

confidence: 99%

“…Each spectrum had an accumulation time of 6-20 seconds and was passed through the D 0 filter to find the best representative average spectrum. The shortest time 1 minute per degree for collection was chosen to prevent sample evaporation and drying 15 . Samples were taken in a small glass cuvette and were placed in the sample chamber of the microscope.…”

Section: Raman Spectroscopymentioning

confidence: 99%

“…Compressibility of BLES and BLES serum or cholesterol or LDL films were calculated by obtaining C 15 values as discussed elsewhere 15,17,19 . Briefly, the C 15 values suggest the total film area compressibility required to drop the surface tension of the films by 15 mN/m during film compression from an initial value of 45 mN/m Fig.…”

Section: Langmuir Surface Balance Studiesmentioning

confidence: 99%

“…These trends were consistent with a change in conformation of the lipids as a function of temperature, where an increased fluidity of the hydrocarbon chain or gauche conformation had been observed at higher temperatures. At lower temperatures [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] however, the hydrocarbon…”

Section: Raman Spectroscopy Studiesmentioning

confidence: 99%

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Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

et al. 2014

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Section: Methodsmentioning

confidence: 99%

Section: Adsorption Kinetics Studiesmentioning

confidence: 99%

Section: Raman Spectroscopymentioning

confidence: 99%

Section: Langmuir Surface Balance Studiesmentioning

confidence: 99%

Section: Raman Spectroscopy Studiesmentioning

confidence: 99%

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Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

et al. 2014

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Impact of Ionic Liquids on the Physicochemical Behavior of Vesicles

Manna,

Barai,

Mandal

et al. 2024

J. Phys. Chem. B

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The effects of two ionic liquids (ILs), 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF 4 ) and 1-butyl-1-methyl pyrrolidinium tetrafluoroborate ([bmp]BF 4 ), on a mixture of phospholipids (PLs) 1,2-dipalmitoyl-sn-glycero-3phosphatidylcholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), and 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) (6:3:1, M/M/M, 70% PL) in combination with 30 mol % cholesterol (CHOL) were investigated in the form of a solvent-spread monolayer and bilayer (vesicle). Surface pressure (π)−area (A) isotherm studies, using a Langmuir surface balance, revealed the formation of an expanded monolayer, while the cationic moiety of the IL molecules could electrostatically and hydrophobically bind to the PLs on the palisade layer. Turbidity, dynamic light scattering (size, ζ-potential, and polydispersity index), electron microscopy, small-angle X-ray/neutron scattering, fluorescence spectroscopy, and differential scanning calorimetric studies were carried out to evaluate the effects of IL on the structural organization of bilayer in the vesicles. The ILs could induce vesicle aggregation by acting as a "glue" at lower concentrations (<1.5 mM), while at higher concentrations, the ILs disrupt the bilayer structure. Besides, ILs could result in the thinning of the bilayer, evidenced from the scattering studies. Steady-state fluorescence anisotropy and lifetime studies suggest asymmetric insertion of ILs into the lipid bilayer. MTT assay using human blood lymphocytes indicates the safe application of vesicles in the presence of ILs, with a minimal toxicity of up to 2.5 mM IL in the dispersion. These results are proposed to have applications in the field of drug delivery systems with benign environmental impact.

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Pulmonary Surfactant: A Unique Biomaterial with Life-saving Therapeutic Applications

Pioselli

Salomone

Mazzola

et al. 2022

CMC

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: Pulmonary surfactant is a complex lipoprotein mixture secreted into the alveolar lumen by type 2 pneumocytes, which is composed by tens of different lipids (approximately 90% of its entire mass) and surfactant proteins (approximately 10% of the mass). It is crucially involved in maintaining lung homeostasis by reducing the values of alveolar liquid surface tension close to zero at end-expiration, thereby avoiding the alveolar collapse, and assembling a chemical and physical barrier against inhaled pathogens. A deficient amount of surfactant or its functional inactivation is directly linked to a wide range of lung pathologies, including the neonatal respiratory distress syndrome. This paper reviews the main biophysical concepts of surfactant activity and its inactivation mechanisms, and describes the past, present and future roles of surfactant replacement therapy, focusing on the exogenous surfactant preparations marketed worldwide and new formulations under development. The closing section describes the pulmonary surfactant in the context of drug delivery. Thanks to its peculiar composition, biocompatibility, and alveolar spreading capability, the surfactant may work not only as a shuttle to the branched anatomy of the lung for other drugs but also as a modulator for their release, opening to innovative therapeutic avenues for the treatment of several respiratory diseases.

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Impairing effect of fibrinogen on the mono-/bi-layer form of bovine lung surfactant

Cited by 3 publications

References 31 publications

Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

Impact of Ionic Liquids on the Physicochemical Behavior of Vesicles

Pulmonary Surfactant: A Unique Biomaterial with Life-saving Therapeutic Applications

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