Impaired T-cell regulation of B-cell growth in Helicobacter pylori–related gastric low-grade MALT lymphoma

D’Elios, Mario Milco; Amedei, Amedeo; Manghetti, M.; Costa, Francesco; Baldari, Cosima T.; Quazi, Adnan S.; Telford, John L.; Romagnani, Sergio; Prete, Gianfranco Del

doi:10.1016/s0016-5085(99)70395-1

Cited by 100 publications

(85 citation statements)

References 30 publications

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“…In addition to promoting the proliferation and differentiation of malignant B-cell clones, tumor-infiltrating T-cells in lowgrade gastric MALT lymphomas are found to be defective in both perforin-mediated cytotoxicity and Fas-Fas ligand mediated apoptosis [28] . On the other hand, the tumor tissues of high-grade MALT lymphomas appear to contain a much higher number of apoptotic lymphoma cells and CD8+ cytotoxic T lymphocytes (CTLs) [21] .…”

Section: Discussionmentioning

confidence: 99%

Expression of CD86 and increased infiltration of NK cells are associated withHelicobacter pylori-dependent state of early stage high-grade gastric MALT lymphoma

Kuo

Chen

Chen³

et al. 2005

WJG

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Abstract Abstract AbstractAIM: A high percentage of early-stage high-grade gastric mucosa-associated lymphoid tissue (MALT) lymphomas remain Helicobacter pylori (H pylori)-dependent. However, unlike their low-grade counterparts, high-grade gastric MALT lymphomas may progress rapidly if unresponsive to H pylori eradication. It is mandatory to identify markers that may predict the H pylori-dependent status of these tumors. Proliferation of MALT lymphoma cells depends on cognate help and cell-to-cell contact of H pylori-specific intratumoral T-cells. To examine whether the expression of co-stimulatory marker CD86 (B7.2) and the infiltration of CD56 (+) natural killer (NK) cells can be useful markers to predict H pylori-dependent status of high-grade gastric MALT lymphoma. METHODS:Lymphoma biopsies from 26 patients who had participated in a prospective study of H pylori-eradication for stage I E high-grade gastric MALT lymphomas were evaluated. Tumors that resolved to Wotherspoon grade II or less after H pylori eradication were classified as H pyloridependent; others were classified as H pylori-independent. The infiltration of NK cells and the expression of CD86 in pre-treatment paraffin-embedded lymphoma tissues were determined by immunohistochemistry. RESULTS:There were 16 H pylori-dependent and 10 H pylori-independent cases. CD86 expression was detected in 11 (68.8%) of 16 H pylori-dependent cases but in none of 10 H pylori-independent cases (P = 0.001). H pylori-dependent high-grade gastric MALT lymphomas contained significantly higher numbers of CD56 (+) NK cells than H pylori-independent cases (2.8±1.4% vs 1.1±0.8%; P = 0.003). CD86 positive MALT lymphomas also showed significantly increased infiltration of CD56 (+) NK cells compared to CD86-negative cases (2.9±1.1% vs 1.4±1.3%; P = 0.005). CONCLUSION:These results suggest that the expression of co-stimulatory marker CD86 and the increased infiltration of NK cells are associated with H pylori-dependent state of early-stage high-grade gastric MALT lymphomas.

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Section: Discussionmentioning

confidence: 99%

Expression of CD86 and increased infiltration of NK cells are associated withHelicobacter pylori-dependent state of early stage high-grade gastric MALT lymphoma

Kuo

Chen

Chen³

et al. 2005

WJG

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“…T cell blasts from each clone were cocultured with 51 Cr-labeled Jurkat cells at an effector͞ target ratio of 10, 5, and 2.5 to 1 for 18 h in the presence of phorbol-12-myristate-13-acetate (10 ng͞ml) and ionomycin (1 mmol͞l), as reported (18). To block Fas-Fas ligand interaction, the anti-Fas antagonistic monoclonal antibody M3 (Immunex) was used at a 5 g͞ml final concentration in a 30-min pretreatment of 51 Cr-labeled Jurkat cells, as reported (22).…”

Section: Methodsmentioning

confidence: 99%

T helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions

Benagiano

Azzurri

Ciervo

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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146

124

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Atherosclerotic lesions are infiltrated by macrophages and T lymphocytes, potentially reactive to pathogens. We studied in vivo activated T lymphocytes that infiltrate atherosclerotic plaques of Helicobacter pylori-infected patients with or without anti-Chlamydia pneumoniae antibodies. In all atherosclerotic lesions, T helper type 1 (Th1) cells were predominant. C. pneumoniae-specific T cells were detected only in the plaques of anti-C. pneumoniae seropositive patients, whereas H. pylori-specific T cells were found in the gastric mucosa but not in the plaques of the same patients. Plaque-derived Th1 cells expressed cytotoxicity, proapoptotic activity, and help for monocyte tissue factor production. Although multifactorial, atherosclerosis can be regarded as a Th1-driven immunopathological condition.

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“…T cell blasts from each clone were co-cultured with 51 Cr-labeled Jurkat cells at eVector to target ratios of 10, 5, and 2.5-1 for 18 h in the presence of PMA (10 ng/ml) and ionomycin (1 mmol/l), as reported [4]. To block Fas-FasL interaction, the anti-Fas antagonistic monoclonal antibody M3 (Amgen, Thousand Oaks, USA) was used at a 5 g/ml Wnal concentration in a 30-min pretreatment of 51 Cr-labeled Jurkat cells, as reported [3].…”

Section: Perforin-mediated Cytotoxicity and Fas-fas Ligand(l)-mediatementioning

confidence: 99%

“…Since most of antigen-activated Th1 and Th0 clones express perforin-mediated cytotoxicity against autologous antigen-presenting APC (e.g., GCAA peptidepulsed B cells) [3], we assessed the cytolytic potential of peptide-speciWc T cell clones using GCAA peptide-pulsed 51 Cr-labeled autologous EBV-B cells as targets. At an eVector to target ratio of 10:1, all 50 CD4 + Th1 and 11 out of the 15 Th0 CD4 + clones lysed GCAA peptide-presenting autologous EBV-B cells (Fig.…”

Section: Gcaa Peptide-speciwc T Cells Inwltrate the Neoplastic Tissuementioning

confidence: 99%

Characterization of tumor antigen peptide-specific T cells isolated from the neoplastic tissue of patients with gastric adenocarcinoma

Amedei

Niccolai

Bella

et al. 2009

Cancer Immunol Immunother

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Gastric cancer is a signiWcant cause of morbidity and mortality worldwide. Surgical resection remains the primary curative treatment for gastric adenocarcinoma, but the poor (15-35%) survival rate at 5 years has prompted many studies for new therapeutic strategies, such as speciWc immunotherapy. The aim of this study was to analyze the functional properties of the T cell response to diVerent antigen peptides related to gastric cancer in patients with gastric adenocarcinoma. To this purpose, we have cloned and characterized tumor-inWltrating T cells (TILs) isolated from the neoplastic gastric tissue samples. A T cell response speciWc to diVerent peptides of gastric cancer antigens tested was documented in 17 out of 20 patients, selected for their HLA-A02 and/or -A24 alleles. Most of the cancer peptidespeciWc TILs expressed a Th1/Tc1 proWle and cytotoxic activity against target cells. The eVector functions of cancer peptide-speciWc T cells obtained from the peripheral blood of the same patients were also studied. The majority of peripheral blood peptide-speciWc T cells also expressed the Th1/Tc1 functional proWle. In conclusion, in most of the patients with gastric adenocarcinoma, a speciWc type-1 T cell response to gastric cancer antigens was detectable and would have the potential of hamper tumor cell growth. However, in order to get tumor cell killing in vivo, the activity and the number of cancer peptide-speciWc Th1/Tc1 cells probably need to be enhanced by vaccination with the appropriate cancer antigenic peptides or by injection of the autologus tumor peptide-speciWc T cells expanded in vitro.

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Impaired T-cell regulation of B-cell growth in Helicobacter pylori–related gastric low-grade MALT lymphoma

Cited by 100 publications

References 30 publications

Expression of CD86 and increased infiltration of NK cells are associated withHelicobacter pylori-dependent state of early stage high-grade gastric MALT lymphoma

Expression of CD86 and increased infiltration of NK cells are associated withHelicobacter pylori-dependent state of early stage high-grade gastric MALT lymphoma

T helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions

Characterization of tumor antigen peptide-specific T cells isolated from the neoplastic tissue of patients with gastric adenocarcinoma

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