Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: Improvement during vitamin E supplementation

Winklhofer‐Roob, Brigitte M.; Ziouzenkova, Ouliana; Puhl, Herbert; Ellemunter, Helmut; Greiner, Peter; Müller, G.; Hof, M.A. van ’t; Esterbauer, Hermann; Shmerling, D. H.

doi:10.1016/0891-5849(95)00063-4

Cited by 58 publications

(38 citation statements)

References 25 publications

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“…In ex vivo studies the low-density lipoprotein (LDL) of patients of this study, when efficiently supplemented with vitamin E, showed normal resistance to a defined oxidative stress (30). In vitro experiments identified 40 /xmol/L as the minimum concentration of ascorbic acid that significantly in hibited oxidative modification of LDL (11), Indeed, the ma jority of CF patients whose LDL was tested showed plasma vitamin C concentrations 40 /xmol/L.…”

Section: Discussionmentioning

confidence: 99%

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation

Winklhofer‐Roob

Ellemunter

Frühwirth

et al. 1997

The American Journal of Clinical Nutrition

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Vitamin C status and possible associations with the disease process in cystic fibrosis (CP) patients were investi gated. Plasma vitamin C concentrations in patients from two different mid-European populations (Swiss, n = 62; Austrian, /j -60) taking no or low-dose vitamin C from multivitamin supplements did not differ from each oilier or from control subjects (» ~ 34). Vitamin C concentrations decreased with age (5,05 /xmol ■ L 1 • y~ '). When followed up for 12 mo, patients had the highest plasma vitamin C concentrations in February and the lowest in May and August (P < 0.01); the decrease in vitamin C was accompanied by increases in plasma malondialdehyde (P < 0.001) and tumor necrosis factor a concentrations (P < 0.01). During supplementation with vitamin E for 2 mo or /3-carotene for 12 mo vitamin C concentrations did not change. They correlated inversely with white blood cell count (r --0.36, P = 0.008), bands (r --0.36, P = 0.02), a j-acid glycoprotein (/* --0.45, P = 0.002), interleukin 6 (r = -0.46, P = 0.0006), and neutrophil elastase/a,-proteinase inhibitor complexes (r = -0.34, P ~ 0.02). In patients with vitamin C concentrations < 40 /xmol/L, all in dexes of inflammation were relatively high, whereas those with concentrations > 80 /¿mol/L (upper quartile of control subjects) showed clearly lower values. These results are consistent with the hypothesis that by scavenging oxygen free radicals vitamin C interacts with an in lì am mat ion-amplifying cycle of activation of alveolar macrophages and neutrophils, release of proinflammatory cytokines and oxygen free radicals, and inactivation of anti pro teases, Am J Clin Nutr 1997;65:1858-66.

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Section: Discussionmentioning

confidence: 99%

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation

Winklhofer‐Roob

Ellemunter

Frühwirth

et al. 1997

The American Journal of Clinical Nutrition

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“…Of the fatty acids, polyunsaturated fatty acids are the most susceptible to oxidation (Bonanome et al, 1992;Reaven et al, 1993), and a diet enriched with linoleic acid increases LDL oxidation in both human (Berry et al, 1991;Reaven et al, 1991;Bonanome et al, 1992;Abbey et al, 1993;Corboy et al, 1993;Reaven et al, 1993;Reaven et al, 1994;Winklerhofer-Roob et al, 1995;Louheranta et al, 1996) and animal experiments (Thomas & Rudel, 1996). Lag time before the onset of the propagation phase of oxidation in vitro has been shown to be shortened (Abbey et al, 1993) or not altered (Reaven et al, 1991) by a linoleic acid rich diet.…”

Section: Introductionmentioning

confidence: 99%

Dietary habits affect the susceptibility of low‐density lipoprotein to oxidation

et al. 1999

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Objective: To study, if there are differences in the fatty acid composition of low-density lipoprotein (LDL) in people eating three different long-standing habitual diets: vegetarian, high ®sh intake, or high saturated fat (milk fat) diet as a control group, and to study if these differences in¯uence the oxidation susceptibility of LDL. Design: Cross-sectional study using blood samples and a validated dietary frequency questionnaire with illustrations. Setting: Helsinki University Central Hospital, Finland. Subjects: The effect of three different types of long-standing diets of different fatty acid content (a strict vegetarian diet, n 11; a high ®sh intake diet, n 9; and a high saturated fat (milk fat) diet, controls, n 7) on the serum and LDL fatty acid content, and on the susceptibility of LDL to oxidation in vitro, was studied in healthy normocholesterolemic volunteers who had been on these diets for years. Oxidation of LDL was carried out by using CuSO 4 as a pro-oxidant. Results: There were no statistically signi®cant differences in the serum lipids or lipoproteins, though the vegetarian group exhibited lowest mean values of total, high-density lipoprotein (HDL) and LDL cholesterol levels. Both the serum and LDL eicosapentaenoic, docosapentaenoic and docosahexaenoic acid proportions were highest in the ®sh and lowest in the vegetarian groups. Linoleic acid was highest among the vegetarians. In the ®sh group, the vitamin A concentration in serum was higher than in vegetarians and controls and b-carotene lower than in controls, but in a-tocopherol, or lycopene concentrations there were no statistically signi®cant differences. The lag phase of LDL oxidation was shortest (116 min) in the ®sh group and longest (165 min) in the vegetarian group, and the control group was between them (129 min). The mean oxidation percentage after 2.5 h of copper-induced oxidation was highest (44%) in the ®sh group and lowest (22%) in the vegetarian group and intermediate (31%) in the control group. Conclusion: Long-term dietary habits predict the fatty acid composition of serum and LDL, and in¯uence the susceptibility of LDL to oxidation. In the ®sh group with the highest content of omega-3 fatty acids in LDL, the oxidation susceptibility of LDL was highest. In the vegetarian group with less omega-3 fatty acids in LDL, the LDL was more resistant to oxidation.

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“…In addition, this exopolysaccharide inhibits opsonic phagocytosis (Pier et al, 2001) and promotes resistance to reactive oxygen molecules (Borriello et al, 2004;Simpson et al, 1989). There is evidence that increased oxidative stress and enhanced lipid peroxidation activity in the CF lung result in the oxidation of membrane phospholipids and low-density lipoprotein (LDL) (Winklhofer-Roob et al, 1995;van der Vliet et al, 1997). These conditions favour the chronic state of infection in CF patients (Ramsey & Wozniak, 2005).…”

Section: Introductionmentioning

confidence: 99%

A novel oxidized low-density lipoprotein-binding protein from Pseudomonas aeruginosa

et al. 2008

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Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: Improvement during vitamin E supplementation

Cited by 58 publications

References 25 publications

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation

Dietary habits affect the susceptibility of low‐density lipoprotein to oxidation

A novel oxidized low-density lipoprotein-binding protein from Pseudomonas aeruginosa

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