Impaired Random Migration and Special Reactivity to Methylprednisolone in Young Guinea Pig Macrophages

Abstract

Macrophage (Mφ) migration ability and its susceptibility to methylprednisolone (MP) action, both in vivo and in vitro, were compared in young and adult guinea pigs. The random migration of oil-induced peritoneal Mφ was tested by the capillary tube method. The obersvation of Mφ locomotion from 4 to 44 h of incubation showed that cells obtained from young animals had a smaller migration ability than those of adults. Besides that, differences in reactivity to MP were observed between the compared groups. The application of MP in vivo led to the stimulation of young Mφ migration in vitro, but inhibited adult Mφ migration. The exposure of adult Mφ to MP in vitro did not affect their motility, but migration of the young Mφ, as previously, was stimulated. Thus, the presented findings report about the immaturity of young Mφ random migration and its special reactivity to MP.