2014
DOI: 10.1371/journal.pone.0092810
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Impaired Mitochondrial Dynamics and Bioenergetics in Diabetic Skeletal Muscle

Abstract: In most cells, mitochondria are highly dynamic organelles that constantly fuse, divide and move. These processes allow mitochondria to redistribute in a cell and exchange contents among the mitochondrial population, and subsequently repair damaged mitochondria. However, most studies on mitochondrial dynamics have been performed on cultured cell lines and neurons, and little is known about whether mitochondria are dynamic organelles in vivo, especially in the highly specialized and differentiated adult skeletal… Show more

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Cited by 116 publications
(120 citation statements)
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“…These high-fat diet-induced changes were paralleled by a 25 % reduction in ADP-stimulated oxygen consumption rate, a lower respiratory control ratio, a reduced ATP production and a reduced mitochondrial content exchange (i.e. the dynamic propagation of a GFP-tagged mitochondrial protein through the mitochondrial network), as observed using confocal microscopy to study mitochondrial dynamics in living animals [16]. Although focused on the liver, another study compared high-fat diets with different fat composition (lard compared with fish-oil) and found that rats fed a high-lard diet, showed an increased number of small and fragmented liver mitochondria as well as reduced Mfn-1, Mfn-2 and Opa1 and increased Drp-1 and Fis1 levels, as compared with both the high fish-oil and chow diet [17].…”
Section: Mitochondrial Dynamics In Lipid Overloadmentioning
confidence: 89%
See 1 more Smart Citation
“…These high-fat diet-induced changes were paralleled by a 25 % reduction in ADP-stimulated oxygen consumption rate, a lower respiratory control ratio, a reduced ATP production and a reduced mitochondrial content exchange (i.e. the dynamic propagation of a GFP-tagged mitochondrial protein through the mitochondrial network), as observed using confocal microscopy to study mitochondrial dynamics in living animals [16]. Although focused on the liver, another study compared high-fat diets with different fat composition (lard compared with fish-oil) and found that rats fed a high-lard diet, showed an increased number of small and fragmented liver mitochondria as well as reduced Mfn-1, Mfn-2 and Opa1 and increased Drp-1 and Fis1 levels, as compared with both the high fish-oil and chow diet [17].…”
Section: Mitochondrial Dynamics In Lipid Overloadmentioning
confidence: 89%
“…Furthermore, in vivo animal studies revealed that 40 weeks of high-fat feeding decreased both protein levels of Mfn-1 and Mfn-2 in skeletal muscle by 20 %, which was associated with a 50 % increase in Fis1 and Drp-1 protein levels [16]. These high-fat diet-induced changes were paralleled by a 25 % reduction in ADP-stimulated oxygen consumption rate, a lower respiratory control ratio, a reduced ATP production and a reduced mitochondrial content exchange (i.e.…”
Section: Mitochondrial Dynamics In Lipid Overloadmentioning
confidence: 99%
“…This location allows mitochondria to communicate with one another via a complex network (100) and to participate in physiological functions by regulated fission and fusion mechanisms, termed "mitochondrial dynamics." Although little is known about mitochondrial dynamics in skeletal muscle, mitochondrial dynamics have been implicated in mitochondrial DNA renewal, cell differentiation and development, mitochondrial respiration, and bioenergetics (46,100,120,129,142).…”
Section: Basic Knowledge Of Myocytes: Importance Of Mitochondriamentioning
confidence: 99%
“…Since the original publication (Karbowski et al, 2004), the mito-PAGFP-based mitochondrial fusion imaging method has been applied to various cell types, including skeletal muscle (Liu et al, 2014), control and LPS+IFNγ-treated astrocytes (Motori et al, 2013), cortical (Berman et al, 2009) and hippocampal neurons (Karbowski et al, 2004), C. elegans (Rolland et al, 2013) and many others. This assay was instrumental in several important discoveries within the field of mitochondrial biology, including the role of mitochondrial fusion in activation of mitochondrial steps in apoptosis (Karbowski et al, 2004; Lee et al, 2004), participation of Bcl-2 family proteins in mitochondrial morphogenesis (Berman et al, 2009; Karbowski et al, 2006; Perciavalle et al, 2012), stress induced mitochondrial hyperfusion (Tondera et al, 2009), and the recently discovered, novel role of the mitochondrial fusion protein Opa1 in the control of mitochondrial division (Anand et al, 2014).…”
Section: E) Concluding Remarks: Other Potential Applications Of Mito-mentioning
confidence: 99%