Impaired intravascular triglyceride lipolysis constitutes a marker of clinical outcome in patients with stable angina undergoing secondary prevention treatment

Spósito, Andrei C.; Lemos, Pedro A.; Santos, Raúl D.; Hueb, Whady; Vinagre, C.G.; Quintella, Edgard Freitas; Carneiro, Otávio Luiz Gomes; Chapman, M. John; Ramires, José Antônio Franchini; Maranhão, Raul C.

doi:10.1016/j.jacc.2003.11.065

Cited by 32 publications

(25 citation statements)

References 25 publications

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“…Artificial lipid nanoemulsions can be used as probes to detect genetically inherited alterations or evaluate pharmacological, nutritional and other interventions on lipoprotein metabolism (1)(2)(3)(4). A single preparation of these artificial nanoemulsions can be injected into several patients for plasma kinetic studies, whereas only the autologous native lipoprotein can be injected into subjects, due to the risk of infection or immunological reactions resulting from the use of heterologous lipoproteins.…”

Section: Introductionmentioning

confidence: 99%

Effect of a cholesterol-rich diet on the metabolism of the free and esterified cholesterol components of a nanoemulsion that resembles LDL in rabbits

Padoveze

Maniero

Oliveira

et al. 2009

Braz J Med Biol Res

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We have shown that the free cholesterol (FC) and the cholesteryl ester (CE) moieties of a nanoemulsion with lipidic structure resembling low-density lipoproteins show distinct metabolic fate in subjects and that this may be related to the presence of dyslipidemia and atherosclerosis. The question was raised whether induction of hyperlipidemia and atherosclerosis in rabbits would affect the metabolic behavior of the two cholesterol forms. Male New Zealand rabbits aged 4-5 months were allocated to a control group (N = 17) fed regular chow and to a 1% cholesterol-fed group (N = 13) during a 2-month period. Subsequently, the nanoemulsion labeled with 3 H-FC and 14 C-CE was injected intravenously for the determination of plasma kinetics and tissue uptake of the radioactive labels. In controls, FC and CE had similar plasma kinetics (fractional clearance rate, FCR = 0.234 ± 0.056 and 0.170 ± 0.038 h -1 , respectively; P = 0.065). In cholesterolfed rabbits, the clearance of both labels was delayed and, as a remarkable feature, FC-FCR (0.089 ± 0.033 h -1 ) was considerably greater than CE-FCR (0.046 ± 0.010 h -1 ; P = 0.026). In the liver, the major nanoemulsion uptake site, uptake of the labels was similar in control animals (FC = 0.2256 ± 0.1475 and CE = 0.2135 ± 0.1580%/g) but in cholesterol-fed animals FC uptake (0.0890 ± 0.0319%/g) was greater than CE uptake (0.0595 ± 0.0207%/g; P < 0.05). Therefore, whereas in controls, FC and CE have similar metabolism, the induction of dyslipidemia and atherosclerosis resulted in dissociation of the two forms of cholesterol.

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Section: Introductionmentioning

confidence: 99%

Effect of a cholesterol-rich diet on the metabolism of the free and esterified cholesterol components of a nanoemulsion that resembles LDL in rabbits

Padoveze

Maniero

Oliveira

et al. 2009

Braz J Med Biol Res

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“…Lipoproteins can be modeled by artificially made lipid emulsions [3][4][5][6][7]. We have shown that nanoemulsions (LDE) roughly resembling the lipidic structure of LDL particles, as described above, but made without protein, are taken-up by LDL receptors [3,6].…”

Section: Introductionmentioning

confidence: 99%

Deposition of Free Cholesterol in the Blood Vessels of Patients with Coronary Artery Disease: a Possible Novel Mechanism for Atherogenesis

Couto¹,

Dallan²,

Lisboa³

et al. 2007

Lipids

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A cholesterol-rich nanoemulsion (LDE) that mimics the composition of low-density lipoprotein (LDL) acquires apoE in the plasma and is taken-up by the cells by LDL receptors. In this study, to verify whether free cholesterol (FC) and the cholesteryl ester (CE) components of LDL are taken-up differently by the vessels. LDE labeled with (3)H-cholesterol and (14)C-cholesteryl oleate was injected into 20 coronary artery disease patients 24 h before a scheduled myocardial coronary artery bypass grafting. The plasma kinetics of both radiolabels was determined from plasma samples collected over 24 h, and fragments of vessels discarded during surgery were collected and analyzed for radioactivity. LDE FC was removed faster than CE. The radioactive counting of LDE CE was greater than that of LDE FC in the blood, but the uptake of FC was markedly greater than that of CE in all fragments: fivefold greater in the aorta (p = 0.04), fourfold greater in the internal thoracic artery (p = 0.03), tenfold greater in the saphenous vein (p = 0.01) and threefold in the radial artery (p = 0.05). In conclusion, the greater removal from plasma of FC compared with CE and the remarkably greater vessel tissue uptake of FC compared with CE suggests that, in the plasma, FC dissociates from the nanoemulsion particles and precipitates in the vessels. Considering LDE as an artificial nanoemulsion model for LDL, our results suggest that dissociation of FC from lipoprotein particles and deposition in the vessel wall may play a role as an independent mechanism in atherogenesis.

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“…In that study, cholesteryl esters clearance was the only independent predictor of the presence of CAD. In another study from our laboratory (30), it was also shown that a slower remnant removal was related with worse subsequent clinical evolution of postmyocardial infarction patients, as followed by an average period of 4.5 years.…”

Section: Figurementioning

confidence: 77%

Metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein in young obese and normal-weight patients with polycystic ovary syndrome

Rocha

Maranhão

Seydell

et al. 2010

Fertility and Sterility

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Impaired intravascular triglyceride lipolysis constitutes a marker of clinical outcome in patients with stable angina undergoing secondary prevention treatment

Abstract: The current study establishes that delayed intravascular TG lipolysis is a strong and independent predictor of evolution to severe angina among patients undergoing secondary prevention therapy of CAD.

Cited by 32 publications

References 25 publications

Effect of a cholesterol-rich diet on the metabolism of the free and esterified cholesterol components of a nanoemulsion that resembles LDL in rabbits

Effect of a cholesterol-rich diet on the metabolism of the free and esterified cholesterol components of a nanoemulsion that resembles LDL in rabbits

Deposition of Free Cholesterol in the Blood Vessels of Patients with Coronary Artery Disease: a Possible Novel Mechanism for Atherogenesis

Metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein in young obese and normal-weight patients with polycystic ovary syndrome

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