Impaired intestinal mucosal immunity is associated with the imbalance of T lymphocyte sub-populations in intrauterine growth-restricted neonatal piglets

Li, Dong; Zhong, Xiang; Zhang, Lili; Kong, Lei; Kong, Yili; Kou, Tao; Wang, Tian

doi:10.1016/j.imbio.2014.12.017

Cited by 12 publications

(10 citation statements)

References 33 publications

(36 reference statements)

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“…The key cytokines IL-4 and IFN-γ regulate the immune response. Dong et al (2015) found that when the serum IL-2 and IL-10 levels were low in IUGR piglets, the percentage of CD8 was increased in blood and spleen, and intestinal mucosal immunity related to the imbalance of T lymphocyte subsets was reduced. Sorensen et al (2009) found that immunomodulatory nutrition (IMN) increased CD4/CD8 ratio with perioperative cancer patients and alleviated immunosuppression in these patients.…”

Section: Discussionmentioning

confidence: 96%

“…Intrauterine growth restriction (IUGR) is defined as a fetus whose weight is less than two standard deviations of average weight for the same age or the 10th percentile of weight for the age ( Black et al, 2008 , Dong et al, 2015 , Ferguson, 1978 , Raqib et al, 2017 ). The morbidity and mortality of low birth weight (LBW) neonates are known to be high ( Valero De et al, 2004 , Yan et al, 2019 ), and urgently require researchers to investigate the immune function of LBW.…”

Section: Introductionmentioning

confidence: 99%

“… Amarilyo et al (2011) implied that IUGR infants with low immunity were at greater risk of infection. A study reported that impaired intestinal mucosal immune function in IUGR piglets is associated with a T lymphocyte subpopulation imbalance ( Dong et al, 2015 ). The spleen is an important peripheral immune organ, which contains T cells and B cells to produce immune responses ( Bronte and Pittet, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Docosahexaenoic acid activates the Nrf2 signaling pathway to alleviate impairment of spleen cellular immunity in intrauterine growth restricted rat pups

Jiang

Wan

Chen

et al. 2021

Saudi Journal of Biological Sciences

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Intrauterine growth retardation (IUGR) impairs immune function in children. IUGR is associated with an imbalance of oxidative stress and abnormal apoptosis. Therefore, an IUGR rats model was established to determine the antioxidant capacity and apoptosis in newborn IUGR rats and explored whether these effects were regulated after Docosahexaenoic acid (DHA) supplementation to rat pups. First, eight normal-birth-weight (NBW) and eight IUGR neonatal rats (a 10% low-protein diet) were used to obtain the antioxidant capacity and apoptosis in IUGR rat pups. Then, 32 newborn rats were randomly assigned to the normal birth weight (NBW), DHA supplementation for NBW (ND), IUGR, and DHA supplementation for IUGR (ID) groups. Starting from the 7th day after birth, DHA was given to the experimental group and the same volume of distilled water was given to the control group for 21 days. (1) DHA improved the serum and spleen CD4/CD8 ratios and IL-4 and IFN-γ mRNA expression. (2) DHA decreased the level of MDA, but increased T-AOC in serum and spleen. (3) DHA increased the protein expression of Bcl-2 while decreased Bax. (4) DHA increased protein expression of the Nrf2 signaling pathway and the downstream antioxidant genes GSH-PX and CAT. DHA may alleviate the impairment of spleen cellular immunity in IUGR rat pups by inhibiting oxidative stress and apoptosis related to the activation of Nrf2 signaling pathway.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Docosahexaenoic acid activates the Nrf2 signaling pathway to alleviate impairment of spleen cellular immunity in intrauterine growth restricted rat pups

Jiang

Wan

Chen

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

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“…The reverse transcription-polymerase chain reaction (RT-PCR) assays were conducted using the SYBR Premix Ex Taq™ Kit (TaKaRa Biotechnology Co., Ltd., Dalian, China). The RNA quality detection, including the RT-PCR and cDNA synthesis, was used the same methods as described in the previous study ( 19 ). The RT-PCR conditions were as follows: initial denaturation at 95°C for 30 s, followed by 40 cycles of denaturation at 95°C for 5 s and annealing at 60°C for 30 s, and finally extension at 72°C for 30 s. The relative gene expression was calculated using the 2 −ΔΔCT method ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

Impacts of Betaine Addition in Sow and Piglet's Diets on Growth Performance, Plasma Hormone, and Lipid Metabolism of Bama Mini-Pigs

et al. 2021

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The present study evaluated the effects of betaine addition in sow and piglet's diets on growth performance, plasma hormone, and lipid metabolism of Bama mini-pigs. A total of 26 pregnant Bama mini-pigs and 104 weaned piglets were selected and divided into different dietary treatment groups (details in “Materials and Methods”). Blood and muscle samples were collected at 65-, 95-, and 125-day-old, respectively. The results showed that betaine addition in sow-offspring diets increased (P < 0.05) the body weight at 125-day-old, average daily gain from 35- to 65-day-old, and average daily feed intake at 35–65 and 35–95 days old of pigs compared with the control group. Betaine addition in sow-offspring diets increased (P < 0.05) the plasma gastrin level at 95-day-old, while betaine addition in sow diets decreased (P < 0.05) the plasma peptide YY and leptin levels at 65-day-old pigs. In the longissimus dorsi muscle of pigs, betaine addition in sow and sow-offspring diets increased (P < 0.05) the C12:0 content at 65-day-old while decreased at 95-day-old. Moreover, betaine addition in sow-offspring diets increased the C24:0 content and decreased the C18:1n9t content at 125-day-old (P < 0.05). In the biceps femoris muscle, the contents of C12:0 at 65-day-old and C20:4n6 at 125-day-old were decreased (P < 0.05) after the betaine addition in both sow and piglet's diets. In addition, betaine addition in sow diets decreased (P < 0.05) the C20:0 content at 125-day-old, while betaine addition in sow-offspring diets increased the C18:3n6 and decreased C24:0 contents at 65-day-old pigs (P < 0.05). In the psoas major muscle, betaine addition in sow and sow-offspring diets decreased (P < 0.05) the contents of C18:1n9t at 65-day-old and C20:1 at 95-day-old, while betaine addition in sow diets decreased (P < 0.05) the intramuscular fat content at 125-day-old. Moreover, betaine addition in sow-offspring diets was also associated with muscle lipid deposition and metabolisms by regulating the gene expressions related to fatty acid metabolism. These findings suggested that betaine addition in sow-offspring diets could improve the growth performance, whereas betaine addition in both sow and sow-offspring diets could enhance lipid quality by altering plasma hormone level and fatty acid composition and regulating the gene expressions related to fatty acid metabolism.

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“…The reverse transcription-polymerase chain reaction (RT-PCR) assays were conducted using the SYBR Premix Ex Taq TM Kit (TaKaRa Biotechnology Co. Ltd., Dalian, China). The quality detection of RNA, the RT-PCR, and the synthesis of cDNA were used the methods described in the previous study (27). The RT-PCR conditions were as follows: initial denaturation at 95 • C for 30 s, followed by 40 cycles of denaturation at 95 • C for 5 s and annealing at 60 • C for 30 s, finally extension at 72 • C for 30 s. The relative gene expression was calculated using the 2 − CT method (28).…”

Section: Real-time Pcr Analysismentioning

confidence: 99%

Dietary Betaine Addition Alters Carcass Traits, Meat Quality, and Nitrogen Metabolism of Bama Mini-Pigs

et al. 2021

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Betaine is widely used as feed additives in animal husbandry as it can cause many benefits such as improving antioxidant ability, growth performance, and carcass traits. However, there are limited studies about the effects of betaine on the Bama mini-pigs. The present study was conducted to evaluate the effects of dietary betaine on carcass traits, meat quality, and nitrogen metabolism of pigs. Twenty-six pregnant Bama mini-pigs and then 104 weaned piglets were assigned for experimental treatments. The plasma and muscle samples were collected at 65-, 95-, and 125-d-old pigs, respectively. The results showed that betaine addition in the sow-offspring diets increased the lean meat rate in the 65-d-old pigs, whereas carcass weight, carcass yield, and loin-eye area were increased in the 95-d-old pigs, and carcass weight and backfat thickness in the 125-d-old pigs. Dietary betaine addition in the sow-offspring diets increased the contents of plasma Asp of 65-d-old, Met of 95- and 125-d-old, and Sar of 125-d-old pigs. Moreover, betaine addition increased the contents of Met, His, Ile, and Phe in Longissimus thoracis et lumborum, whereas those contents were decreased in biceps femoris and psoas major muscles at different stages. Betaine addition in the sow and piglets' diets regulated the muscle fiber-type and myogenic regulatory gene expressions. In summary, betaine addition in the sow and sow-offspring diets could improve the carcass traits and meat quality by altering the plasma biochemical parameters, amino acid composition, and gene expressions of skeletal muscle.

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Impaired intestinal mucosal immunity is associated with the imbalance of T lymphocyte sub-populations in intrauterine growth-restricted neonatal piglets

Cited by 12 publications

References 33 publications

Docosahexaenoic acid activates the Nrf2 signaling pathway to alleviate impairment of spleen cellular immunity in intrauterine growth restricted rat pups

Docosahexaenoic acid activates the Nrf2 signaling pathway to alleviate impairment of spleen cellular immunity in intrauterine growth restricted rat pups

Impacts of Betaine Addition in Sow and Piglet's Diets on Growth Performance, Plasma Hormone, and Lipid Metabolism of Bama Mini-Pigs

Dietary Betaine Addition Alters Carcass Traits, Meat Quality, and Nitrogen Metabolism of Bama Mini-Pigs

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