Impaired insulin-mediated glucose uptake in monocytes of short children with intrauterine growth retardation

Cutfield, Wayne S.; Luk, Wing Hang; Skinner, S.J.M.; Robinson, Elizabeth

doi:10.1046/j.1399543x.2000.010403.x

Cited by 2 publications

(2 citation statements)

References 34 publications

(60 reference statements)

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“…However, this study did not examine monocyte subpopulations and separation of monocytes from whole blood can potentially alter expression of certain cell surface molecules 19 . Radioactive glucose tracers have also been used to measure monocyte uptake of glucose 20,21 , but monocytes must be previously isolated for this method and usage of radioactivity requires significant safety precautions. Our protocol uses routine biosafety procedures and is minimally manipulative, thus allowing for the flow cytometric measurement of 2-NBDG uptake by monocytes and monocyte subpopulations mimicking in vivo conditions.…”

Section: Representative Resultsmentioning

confidence: 99%

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Palmer¹,

Anzinger²,

Butterfield³

et al. 2016

JoVE

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Monocytes are innate immune cells that can be activated by pathogens and inflammation associated with certain chronic inflammatory diseases. Activation of monocytes induces effector functions and a concomitant shift from oxidative to glycolytic metabolism that is accompanied by increased glucose transporter expression. This increased glycolytic metabolism is also observed for trained immunity of monocytes, a form of innate immunological memory. Although in vitro protocols examining glucose transporter expression and glucose uptake by monocytes have been described, none have been examined by multi-parametric flow cytometry in whole blood. We describe a multi-parametric flow cytometric protocol for the measurement of fluorescent glucose analog 2-NBDG uptake in whole blood by total monocytes and the classical (CD14 ++ CD16 -), intermediate (CD14 ++ CD16 + ) and non-classical (CD14 + CD16 ++) monocyte subpopulations. This method can be used to examine glucose transporter expression and glucose uptake for total monocytes and monocyte subpopulations during homeostasis and inflammatory disease, and can be easily modified to examine glucose uptake for other leukocytes and leukocyte subpopulations within blood.

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Section: Representative Resultsmentioning

confidence: 99%

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Palmer¹,

Anzinger²,

Butterfield³

et al. 2016

JoVE

View full text Add to dashboard Cite

show abstract

“…. Radioactive glucose tracers have also been used to measure monocyte uptake of glucose 20,21 , but monocytes must be previously isolated for this method and usage of radioactivity requires significant safety precautions. Our protocol uses routine biosafety procedures and is minimally manipulative, thus allowing for the flow cytometric measurement of 2-NBDG uptake by monocytes and monocyte subpopulations mimicking in vivo conditions.…”

mentioning

confidence: 99%

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Palmer

Anzinger

Butterfield

et al. 2016

JoVE

View full text Add to dashboard Cite

Monocytes are innate immune cells that can be activated by pathogens and inflammation associated with certain chronic inflammatory diseases. Activation of monocytes induces effector functions and a concomitant shift from oxidative to glycolytic metabolism that is accompanied by increased glucose transporter expression. This increased glycolytic metabolism is also observed for trained immunity of monocytes, a form of innate immunological memory. Although in vitro protocols examining glucose transporter expression and glucose uptake by monocytes have been described, none have been examined by multi-parametric flow cytometry in whole blood. We describe a multi-parametric flow cytometric protocol for the measurement of fluorescent glucose analog 2-NBDG uptake in whole blood by total monocytes and the classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)) and non-classical (CD14(+)CD16(++)) monocyte subpopulations. This method can be used to examine glucose transporter expression and glucose uptake for total monocytes and monocyte subpopulations during homeostasis and inflammatory disease, and can be easily modified to examine glucose uptake for other leukocytes and leukocyte subpopulations within blood.

show abstract

Impaired insulin-mediated glucose uptake in monocytes of short children with intrauterine growth retardation

Cited by 2 publications

References 34 publications

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

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